Characterization of type 3 metallothionein-like gene (OsMT-3a) from rice, revealed its ability to confer tolerance to salinity and heavy metal stresses

Mekawy, Ahmad Mohammad M.; Assaha, Dekoum V. M.; Munehiro, Riko; Kohnishi, Eri; Nagaoka, Toshinori; Ueda, Akihiro; Saneoka, Hirofumi

doi:10.1016/j.envexpbot.2017.12.002

Cited by 54 publications

(30 citation statements)

References 44 publications

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“…The leaf mineral concentration was determined according to Mekawy et al (2018). Leaf blades were dried at 80 C for 1 day and crushed using a μT-48 (TAITEC, Japan).…”

Section: Measurements Of Photosynthetic Activitiesmentioning

confidence: 99%

Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves

Takagi

Miyagi

Tazoe

et al. 2020

Plant Cell & Environment

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Phosphorus (P) is an essential mineral nutrient for plants. Nevertheless, excessive P accumulation in leaf mesophyll cells causes necrotic symptoms in land plants; this phenomenon is termed P toxicity. However, the detailed mechanisms underlying P toxicity in plants have not yet been elucidated. This study aimed to investigate the molecular mechanism of P toxicity in rice. We found that under excessive inorganic P (Pi) application, Rubisco activation decreased and photosynthesis was inhibited, leading to lipid peroxidation. Although the defence systems against reactive oxygen species accumulation were activated under excessive Pi application conditions, the Cu/Zn-type superoxide dismutase activities were inhibited. A metabolic analysis revealed that excessive Pi application led to an increase in the cytosolic sugar phosphate concentration and the activation of phytic acid synthesis. These conditions induced mRNA expression of genes that are activated under metal-deficient conditions, although metals did accumulate. These results suggest that P toxicity is triggered by the attenuation of both photosynthesis and metal availability within cells mediated by phytic acid accumulation. Here, we discuss the whole phenomenon of P toxicity, beginning from the accumulation of Pi within cells to death in land plants.

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“…The leaf mineral concentration was determined according to Mekawy et al (2018). Leaf blades were dried at 80 C for 1 day and crushed using a μT-48 (TAITEC, Japan).…”

Section: Measurements Of Photosynthetic Activitiesmentioning

confidence: 99%

Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves

Takagi

Miyagi

Tazoe

et al. 2020

Plant Cell & Environment

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“…According to Xue et al [27], GhMT3a could function as an effective ROS scavenger in cotton, transgenic tobacco, and yeast, and its expression was induced by various abiotic stresses through ROS signaling. In rice, OsMT-3a plays a crucial role in salinity and heavy metal tolerance via ROS scavenging [28]. In our study, the relative expression of LcCCR2 and LcMT2 was induced by ROS treatment at the early stage of floral induction, but the induction was trapped by the ROS scavenger DMTU, suggesting that they might be involved in ROS-promoted flowering at an early stage of floral induction.…”

Section: Discussionmentioning

confidence: 56%

Genome-Wide Transcriptomic Analysis Reveals a Regulatory Network of Oxidative Stress-Induced Flowering Signals Produced in Litchi Leaves

Lü

et al. 2020

Genes

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Litchi is an important subtropical fruit tree that requires an appropriately low temperature to trigger floral initiation. Our previous studies have shown that reactive oxygen species (ROS) are involved in litchi flowering. To identify oxidative stress-induced flowering related genes in leaves, 'Nuomici' potted trees were grown at medium low-temperature conditions (18/13 • C for day/night, medium-temperature). The trees were treated with the ROS generator methyl viologen dichloride hydrate (MV) as the MV-generated ROS treatment (MM, medium-temperature plus MV) and water as the control treatment (M, medium-temperature plus water). Sixteen RNA-sequencing libraries were constructed, and each library generated more than 5,000,000 clean reads. A total of 517 differentially expressed genes (DEGs) were obtained. Among those DEGs, plant hormone biosynthesis and signal transduction genes, ROS-specific transcription factors, such as AP2/ERF and WRKY genes, stress response genes, and flowering-related genes FLOWERING LOCUS T1 (FT1) and FLOWERING LOCUS T2 (FT2) were significantly enriched. Then, as a confirmatory experiment, the potted trees were uniformly sprayed with MV, N,N'-dimethylthiourea (DMTU, ROS scavenger) plus MV, and water at medium-temperature. The results showed that the MV-generated ROS promoted flowering and changed related gene expression, but these effects were repressed by DMTU treatment. The results of our studies indicate that ROS could promote flowering and partly bypass chilling for litchi flowering.

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“…We will be investigating such effects in subsequent works. Overexpression could be used to analysis the function of mutated genes . In our study, seven mutant genes are found to be related to high methanol tolerability by gene overexpression.…”

Section: Discussionmentioning

confidence: 79%

Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution

et al. 2018

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Methylobacterium extorquens AM1, which can be used as a methylotrophic cell factory (MeCF) for the production of fine chemicals from methanol, is the most extensively studied model methylotrophic strain. However, its low tolerance for methanol limits the development of bioprocesses and there have been no reports of improved methanol tolerance of M. extorquens AM1. In this study, atmospheric and room temperature plasma (ARTP) mutagenesis, in combination with adaptive laboratory evolution (ALE), is used to generate a mutant with high methanol tolerance (referred to as CLY-2533). The final cell density of CLY-2533 is 7.10 times higher than that of the wild-type strain in medium containing 5% (v/v) methanol. Through comparative genomics analysis and overexpression of the exploited putative genes, seven mutated genes are identified as being closely related to the higher methanol tolerance of CLY-2533. Additionally, the mvt operon, which contains genes related to the biosynthesis of mevalonate acid (MEV), is introduced into CLY-2533. This recombinant strain shows significant improvements in both MEV production and cell growth in 5% methanol medium. These findings will be helpful in rational design of methanol-utilizing strain for an improved host platform for methanol based biomanufacturing.

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Characterization of type 3 metallothionein-like gene (OsMT-3a) from rice, revealed its ability to confer tolerance to salinity and heavy metal stresses

Cited by 54 publications

References 44 publications

Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves

Phosphorus toxicity disrupts Rubisco activation and reactive oxygen species defence systems by phytic acid accumulation in leaves

Genome-Wide Transcriptomic Analysis Reveals a Regulatory Network of Oxidative Stress-Induced Flowering Signals Produced in Litchi Leaves

Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution

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