Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformis

Orth, Gérard; Jabłońska, Stefania; Favre, Michel; Croissant, O; Jarzabek-Chorzelska, M; Rzesa, G

doi:10.1073/pnas.75.3.1537

Cited by 276 publications

(163 citation statements)

References 22 publications

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“…First, there was some initial concern over the Washington B strain of virus as a probe for virus DNA in the Vx-7 tumour. This was an important potential difficulty since it has been shown that strains with significantly different antigenic and nucleotide compositions exist in both human (Gissmann & zur Hausen, 1976;Orth et al, 1977Orth et al, , 1978Pfister & Gissmann, 1978;Heilman et al, 1980) and bovine (Lancaster & Olson, 1978;Lancaster, 1979;Law et al, 1979;Pfister et al, 1979) papilloma viruses. In addition, the opportunity for selection, over time, of unique virus sequences in Vx-7 was a definite possibility.…”

Section: Discussionmentioning

confidence: 99%

Integrated Shope Virus DNA is Present and Transcribed in the Transplantable Rabbit Tumour Vx-7

McVay¹,

Fretz²,

Wettstein³

et al. 1982

Journal of General Virology

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SUMMARYTo facilitate a molecular analysis of Shope papilloma virus-induced neoplastic cells, we have established a cell line from Vx-7, a transplantable tumour originally induced by the Shope virus. Single phase molecular hybridization and Southern transfer methods were employed to assess copy number and physical state of the DNA, and the extent of transcription. Both tumour and cell line were found to contain multiple copies of the virus genome and these were all integrated into the host cell DNA. Transcripts corresponding to a complexity of approx. 1% of the virus genome were detected at low abundance. These results are discussed relative to our earlier findings with tumours induced directly by virus, and to requirements for maintenance of the Vx-7 tumour over the 30 years that it has been in existence.

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Section: Discussionmentioning

confidence: 99%

Integrated Shope Virus DNA is Present and Transcribed in the Transplantable Rabbit Tumour Vx-7

McVay¹,

Fretz²,

Wettstein³

et al. 1982

Journal of General Virology

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“…3 NMSC are often found on sun-exposed sites in patients with the rare inherited genetic disease epidermodysplasia verruciformis (EV). 4 These patients are highly susceptible to human papillomavirus (HPV) infections 5,6 mainly by the Beta-papillomaviruses, a phylogenetic subgroup of cutaneous HPVs (e.g., HPV5 and HPV8). 7 These HPV types are detectable in skin carcinomas, as well as in control specimens of healthy skin from the general population.…”

mentioning

confidence: 99%

Subtype HPV38b[FA125] demonstrates heterogeneity of human papillomavirus type 38

Hazard

Eliasson

Dillner

et al. 2006

Intl Journal of Cancer

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The human papillomaviruses (HPVs) exist as more than 100 distinct types. While variants of HPV are common, only few HPV subtypes have been reported. HPV type 38 has been proposed to be associated with nonmelanoma skin cancer (NMSC), with reported prevalences of up to 55%. A subtype of HPV38 was cloned, completely sequenced and found to have a 96% sequence similarity to prototype HPV38 in the L1 open reading frame. The presence of prototype HPV38 and HPV38b[FA125] was examined in paired biopsies of tape-stripped skin lesions and healthy skin from 269 immunocompetent patients by real-time PCR. Prototype HPV38 and HPV38b[FA125] were present in seven (3%) and five (2%) lesions, respectively, in viral loads ranging from one copy per 150 cells to one copy per 70,000 cells. In summary, we found that HPV38 is heterogeneous and is one of so far only few HPVs that contain subtypes. The heterogeneity needs to be considered in studies of the biology of this virus. ' 2006 Wiley-Liss, Inc.Key words: HPV; subtype; nonmelanoma skin cancer; multiplyprimed rolling circle amplification Nonmelanoma skin cancers (NMSC) are the most prevalent malignancies in the Caucasian population 1 and an increase in prevalence has been seen in European countries during the last few years. 2 NMSC comprises basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). BCC has a relatively good prognosis, while SCC accounts for up to 20% of all deaths from skin cancer. 3 NMSC are often found on sun-exposed sites in patients with the rare inherited genetic disease epidermodysplasia verruciformis (EV). 4 These patients are highly susceptible to human papillomavirus (HPV) infections 5,6 mainly by the Beta-papillomaviruses, a phylogenetic subgroup of cutaneous HPVs (e.g., HPV5 and HPV8). 7 These HPV types are detectable in skin carcinomas, as well as in control specimens of healthy skin from the general population. [8][9][10][11][12] There are more than 100 different HPV types that have been completely sequenced. 7 In addition, there appears to exist about 100 additional putative HPV types, with sequence information so far available only from PCR amplimers. 8,[13][14][15][16] Variants of HPV types contain more than 98% sequence homology in the L1 open reading frame (ORF). 7 Variants is a common finding that appears to associate with ethnic groups and sometimes even with biologic behavior of the virus. 17,18 By contrast, subtypes containing 90-98% sequence homology in the L1 region are very rare, with HPV 68a/HPV68b, HPV46, HPV55 and HPV64 (now known to be subtypes, originally thought to be different viruses) being the only fully characterized examples. 7,17 Although the reasons for this are not known, existence of subtypes has profound implications for design of virus detection systems and for understanding of the biology of the viruses.Epidemiological studies have established a causal association between UV-radiation and NMSC, 3 but the role of HPV in these cancers remains to be elucidated.A recent study 19 has demonstrated that the early proteins E...

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“…Epidermodysplasia Verruciformis (EV) is a chronic skin disease caused by human papilloma virus (HPV) (Lewandowsky & Lutz, 1922;Orth et al, 1978). EV converts to squamous cell carcinoma in 30 % of the cases (Jablonska et al, 1972;zur Hausen, 1977;Lutzner, 1978;Orth et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

“…The HPV DNA was molecularly cloned in Escherichia coli X1776 using plasmid pBR325. We have constructed a detailed physical map of the cloned DNA, which should be useful for examining the relationship of HPV and skin carcinoma.Epidermodysplasia Verruciformis (EV) is a chronic skin disease caused by human papilloma virus (HPV) (Lewandowsky & Lutz, 1922;Orth et al, 1978). EV converts to squamous cell carcinoma in 30 % of the cases (Jablonska et al, 1972;zur Hausen, 1977;Lutzner, 1978;Orth et al, 1979).…”

mentioning

confidence: 99%

“…EV converts to squamous cell carcinoma in 30 % of the cases (Jablonska et al, 1972;zur Hausen, 1977;Lutzner, 1978;Orth et al, 1979). The HPV DNAs isolated from flat warts or macular lesions of EV patients have been analysed using restriction endonucleases, and several types of HPV, including 3, 5, 8, and 9, have been found in EV lesions (Orth et al, 1978;Pfister et al, 1981;Green et al, 1982;Kremsdorf et al, 1982). HPV-5 DNA has been reported to exist in carcinoma cells as unintegrated, free viral DNA (Orth et al, 1980;Ostrow et al, 1982).…”

mentioning

confidence: 99%

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Molecular Cloning of a New Human Papilloma Virus Isolated from Epidermodysplasia Verruciformis Lesions

Tsumori

Yutsudo

Nakano

et al. 1983

Journal of General Virology

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SUMMARYWe have recently reported isolation of a new human papilloma virus (HPVNF) from red plaques of a patient (NF) with epidermodysplasia verruciformis. The HPV DNA was molecularly cloned in Escherichia coli X1776 using plasmid pBR325. We have constructed a detailed physical map of the cloned DNA, which should be useful for examining the relationship of HPV and skin carcinoma.Epidermodysplasia Verruciformis (EV) is a chronic skin disease caused by human papilloma virus (HPV) (Lewandowsky & Lutz, 1922;Orth et al., 1978). EV converts to squamous cell carcinoma in 30 % of the cases (Jablonska et al., 1972;zur Hausen, 1977;Lutzner, 1978;Orth et al., 1979). The HPV DNAs isolated from flat warts or macular lesions of EV patients have been analysed using restriction endonucleases, and several types of HPV, including 3, 5, 8, and 9, have been found in EV lesions (Orth et al., 1978;Pfister et al., 1981;Green et al., 1982;Kremsdorf et al., 1982). HPV-5 DNA has been reported to exist in carcinoma cells as unintegrated, free viral DNA (Orth et al., 1980;Ostrow et al., 1982). Although HPV is strongly suspected of being involved in carcinogenesis, little is known about this virus in comparison with other papovaviruses such as polyoma virus and SV40. This is due in part to the narrow host range of HPV and absence of a system for virus propagation in cultured cells.Recently, we isolated a new HPV from an EV patient (NF), a 24 year-old man (Yutsudo et al., 1982). The cutaneous lesions of NF were red plaques with pityroid scales on the surface. The molecular weight of isolated HPV DNA was 5.0 x 106. The DNA was cleaved at one site with EcoRI or BglI, two sites with HindlII, three sites with BamHI or XbaI, and at more than five sites with HindlI. This cleavage pattern differs from those of other types of HPV reported so far. To elucidate the functions of HPV genes and their role in carcinogenesis, a means of efficiently preparing large amounts of DNA is necessary. Unfortunately, the new virus, like other HPVs, does not replicate in cultured cells. We therefore carried out experiments to clone the DNA of this HPV.HPV DNA used for cloning was extracted from red plaques of patient NF. Form I DNA was purified by caesium chloride-ethidium bromide density gradient centrifugation as described previously (Yutsudo et al., 1982). HPV DNA was cut with EcoRI, producing linear DNA which was then ligated into the EcoRI site of plasmid pBR325 (3.6 x 106 daltons). The single EcoRI site in pBR325 lies within the gene coding for chloramphenicol resistance. After digestion with EcoRI, pBR325 DNA was treated with bacterial alkaline phosphatase. The cleaved HPV and plasmid pBR325 DNAs were mixed at a ratio of 2 : 1 (i.e. 0.2 p.g of HPV DNA and 0.1 lag of pBR325 DNA) in a ligation mixture (20 ~tl) containing 50 mM-Tris-HC1 pH 7.8, 10 mM-MgCI2, 20 mM-dithiothreitol, 1 mM-ATP, 50 ~tg/ml bovine serum albumin, and 3 units ofT4 DNA ligase (New England Biolabs, Beverly, Mass., U.S.A.). After incubation for 15 h at 4 °C, the DNA was precipitated, resuspende...

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Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformis

Cited by 276 publications

References 22 publications

Integrated Shope Virus DNA is Present and Transcribed in the Transplantable Rabbit Tumour Vx-7

Integrated Shope Virus DNA is Present and Transcribed in the Transplantable Rabbit Tumour Vx-7

Subtype HPV38b[FA125] demonstrates heterogeneity of human papillomavirus type 38

Molecular Cloning of a New Human Papilloma Virus Isolated from Epidermodysplasia Verruciformis Lesions

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