Characterization of two parvalbumin genes and their association with growth traits in Asian seabass (Lates calcarifer)

Abstract

Parvalbumins are extremely abundant in fish muscle and play an important role in muscle relaxation. In this study, two parvalbumin genes (PVALB1 and PVALB2) were cloned from Asian seabass (Lates calcarifer). The cDNAs for PVALB1 and PVALB2 were 840 and 667 bp respectively. Both genes consisted of five exons and four introns, encoded 109 amino acids, and were of beta lineage. Using real-time polymerase chain reaction, expression of PVALB1 was detected in all 10 tissues tested, with expression in brain, kidney, muscle and small intestine being 15- to 322-fold higher than in the other tissues. Expression of PVALB2 was detected only in muscle, brain and intestine, and was up to 10-fold lower than PVALB1 expression. A (CT)(17) microsatellite in the 3'-untranslated region of PVALB1 and three single nucleotide polymorphisms (SNPs) in the third intron of PVALB2 were identified. The microsatellite in PVALB1 was significantly associated with body weight and body length at 90 days post-hatch (P < 0.01), whereas the SNPs in PVALVB2 were not associated with these traits.

Analysis of Two Lysozyme Genes and Antimicrobial Functions of Their Recombinant Proteins in Asian Seabass

“…It is widely distributed in tropical Indo-west Pacific, and Northern Australia. Since 1998, we started a breeding program for Asian seabass to improve growth [21,22], and developed a number of genomic tools to facilitate the selective breeding program, such as microsatellites [21,23], SNPs in genes [24], BAC and cDNA libraries [25,26], linkage and physical maps [27,28,29], a genetic tracing system [30], and transcriptomes [31]. Diseases are the major bottleneck for sustainable and profitable aquaculture [32].…”

Authentication of closely related scombrid, catfish and tilapia species by PCR-based analysis and isoelectric focusing of parvalbumin

“…The third intron of parvalbumin genes was amplified by means of primers IFF 232 and IFF 233 developed in a previous study [64]; the primers are located in exon three and four of a parvalbumin gene of barramundi, Lates calcarifer [28,65]. PCR conditions: preheating for 5 min/95 °C, followed by 35 cycles of 1 min/94 °C, 1 min/56 °C, 1 min/72 °C and final heating for 7 min at 72 °C; primer concentration: 0.5 μM, DNA concentration: 2 ng/μl.…”

Cloning and characterization of the calreticulin gene in Asian seabass (Lates calcarifer)

“…QTL analysis and association analysis were conducted as previously described Xu et al, 2006). Briefly, QTL analysis was carried out by the use of MapQTL 4.0 (Van Ooijen et al, 2002) with the new genotypes at the CRT locus, and available genotype data of the 117 markers previously genotyped and phenotypic data of the 380 progeny .…”

“…Only a few genes (e.g. GH, IGF receptor, parvalbumin, myostatin, TH1 and TH2) have been cloned and characterized (Yowe and Epping, 1995;Xu et al, 2006;De Santis et al, 2008). However, up to date, there is no report on relationships between genes and cold tolerance in this species.…”