Characterization of two novel lytic bacteriophages for reducing biofilms of zoonotic multidrug-resistant Staphylococcus aureus and controlling their growth in milk

Gharieb, Rasha; Saad, Mai Farag; Mohamed, Asmaa S.; Tartor, Yasmine H.

doi:10.1016/j.lwt.2020.109145

Cited by 23 publications

(21 citation statements)

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“…Lytic bacteriophages have been used as an effective therapeutic strategy to remove biofilm cells. A recently published study proved that two lytic phages vB_SauM_ME18 and vB_SauM_ME126 are potential natural antimicrobials for inhibiting biofilm of MDR S. aureus (Gharieb et al, 2020). Recent investigations have shown that (engineered) phage-derived enzymes-polysaccharide depolymerase or peptidoglycandegrading enzymes-are promising therapeutic anti-biofilm candidates (Reuter and Kruger, 2020).…”

Section: Phage Therapymentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections are not easy to treat due to resident multidrug-resistant microbes. Low efficiency of various treatments and in vivo toxicity of available antibiotics drive the researchers toward the discovery of many effective natural anti-biofilm agents. Natural extracts and natural product-based antibiofilm agents are more efficient than the chemically synthesized counterparts with lesser side effects. The present review primarily focuses on various natural anti-biofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and microbial enzymes along with their sources, mechanism of action via interfering in the quorum-sensing pathways, disruption of extracellular polymeric substance, adhesion mechanism, and their inhibitory concentrations existing in literature so far. This study provides a better understanding that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species. This information can be exploited further to improve the therapeutic strategy by a combination of more than one natural anti-biofilm compounds from diverse sources.

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Section: Phage Therapymentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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“…In a recent study on S. aureus phages, scientists in Egypt identified two lytic phages (vB_SauM_ME126 and vB_SauM_ME18), which were isolated from five different wastewater sources. These phages exhibited activity against multidrug-resistant (MDR) S. aureus , managing to destroy the bacteria at a relatively high rate ( Gharieb et al, 2020 ).…”

Section: Sources Of Bacteriophagesmentioning

confidence: 99%

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Alharbi

Ziadi

2021

Saudi Journal of Biological Sciences

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Antibiotic resistance is a common and serious public health worldwide. As an alternative to antibiotics, bacteriophage (phage) therapy offers one of the best solutions to antibiotic resistance. Bacteriophages survive where their bacterial hosts are found; thus, they exist in almost all environments and their applications are quite varied in the medical, environmental, and industrial fields. Moreover, a single phage or a mixture of phages can be used in phage therapy; mixed phages tend to be more effective in reducing the number and/or activity of pathogenic bacteria than that of a single phage.

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“…The methicillin‐resistant Staphylococcus aureus (MRSA) strains are now found worldwide and most of the countries have difficulties to combat nosocomial infections associated with S. aureus (Gharieb et al . 2020). Numerous options substitute to the conventional antibiotics exists for treating infections, including bacteriophage, predatory microscopic organisms, bacteriocins and antimicrobial peptides (Joerger 2003), but current advancements in the nanotechnology to design nanoparticles with selected physicochemical properties have been paved the way for a novel mark of defence against antibiotic‐resistant micro‐organisms (Huh and Kwon 2011).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of microbial growth by silver nanoparticles synthesized from Fraxinus xanthoxyloides leaf extract

Rafiq

Zahid

Qadir

et al. 2020

J. Appl. Microbiol.

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Aims Conventional antibiotics have been failed to treat infectious diseases due to emergence of multidrug resistance (MDR) in some common pathogens. The current study aimed to formulate new antimicrobials from greener sources. In the midst of these efforts, nanotechnology is a newly emerged field, in which the synthesis of new nanoparticles through novel and efficient means is on the rise. Methods and Results The current work has been carried out to assess the potential of Fraxinus xanthoxyloides (FX) leaf extract in biosynthesis of silver nanoparticles (FX‐AgNPs). This method is economical and simple one‐step approach to synthesize AgNPs. Characterization of FX‐AgNPs has been done by UV‐Visible spectroscopy, scanning electron microscope (SEM), X‐ray diffraction (XRD), transmission electronic microscope (TEM) and Fourier transforms infrared spectroscopy (FT–IR). The formation of FX‐AgNPs has confirmed through UV‐Visible spectroscopy (at 430 nm) by change of colour owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of FX‐AgNPs has established. Functional groups existing in F. xanthoxyloides leaf extract are confirmed by FT‐IR spectrum. SEM and TEM authenticated morphology of the AgNPs. The newly synthesized nanoparticles were evaluated for their antimicrobial potential. Minimum inhibitory concentration was determined against Escherichia coli, methicillin‐resistant Staphylococcus aureus (MRSA) strains, Pseudomonas aeruginosa and Candida albicans by microtiter plate assay. The lowest inhibition (69%) observed against MRSA was at a concentration of 50 ppm FX‐AgNPs and maximum inhibition (81%) observed was against P. aeruginosa. The biosynthesized AgNPs triggered up to 68·6% reduction of the P. aeruginosa biofilm when compared to the control. Conclusion It can be concluded that nanoparticles could be a better alternative of antibiotics with greater efficacies and represent a valuable milestone to fight against infections caused by MDR pathogens. Significance and Impact of the Study This study imparts a useful insight into the development of a new antimicrobial agent from a novel source.

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Characterization of two novel lytic bacteriophages for reducing biofilms of zoonotic multidrug-resistant Staphylococcus aureus and controlling their growth in milk

Cited by 23 publications

References 50 publications

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Inhibition of microbial growth by silver nanoparticles synthesized from Fraxinus xanthoxyloides leaf extract

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