Characterization of two low-potential cytochromes from bean sprouts

Hirasawa, Masakazu; Chang, Kai-Tai; Dose, Michelle M.; Lou, Bih‐Show; Ondrias, M. R.; Knaff, David B.

doi:10.1016/0005-2728(93)90050-p

Cited by 5 publications

(1 citation statement)

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“…To date, no evidence has been presented for the involvement of reduced ferred0xin, as opposed to NADPH, as the immediate electron donor for CO2 assimilation in any oxygenic photosynthetic organism, although the role of ferredoxin-dependent reductive carboxylations in CO2 assimilation during anoxygenic photosynthesis has been well documented [4]. Recent work in our laboratory has demonstrated the presence of ferredoxin, FNR and two low-potential cytochromes in bean sprouts, a nonphotosynthetic tissue [5][6][7]. Below, as part of our continuing characterization of the role of ferredoxin in bean sprouts, we present evidence that suggests that bean sprouts possess the *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Ferrodoxin‐dependent CO₂ fixation in bean sprouts

et al. 1995

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Extracts of bean sprouts are capable of reducing ferredoxin and of catalyzing the incorporation of bicarbonate and acetyl coenzyme A into an organic compound that is likely to be pyruvate, in a reaction that requires reduced ferredoxin. The rate of the reaction, the first known for which ferredoxin appears to serve as the direct reductant for CO 2 fixation in a higher plant, depends on the concentrations of both ferredoxin and bicarbonate, with half-maximal rates being observed at ferredoxin and bicarbonate concentrations of 0.8 ttM and 200/~M, respectively.

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