Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

Lü, Feng-Gong; Fu, Kai‐Yun; Guo, Wen‐Chao; Li, Guoqing

doi:10.1016/j.gene.2015.06.032

Cited by 32 publications

(25 citation statements)

References 59 publications

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“…Instead, JHEH is a member of the microsomal epoxide hydrolases, which are non-secreted enzymes, active in different organs and tissues. JHEH is responsible for irreversibly opening the epoxide ring of JH to produce JH diol 10,11 . Finally, JHDK catalyzes the phosphorylation of JH diol to produce a more water-soluble metabolite 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

Tusun

Liang

et al. 2017

Sci Rep

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Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. The results reveals a conserved catalytic mechanism of AlucJHEH toward JH. Our study also demonstrates that the mRNA of AlucJHEH gene was detectable in head, thorax and abdomen from all life stages. To functionally characterize the AlucJHEH gene, three fragments of double-stranded RNAs (dsRNAs) were designed to target different regions of the sequence. Injection of 3rd nymphs with dsRNA fragments successfully knocked down the target gene expression, and a significantly decreased survival rate was observed, together with a molting block, These findings confirm the important regulatory roles of AlucJHEH in A. lucorum and indicate this gene as a promising target for future hemipterans pest control.

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Section: Introductionmentioning

confidence: 99%

Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

Tusun

Liang

et al. 2017

Sci Rep

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“…JHEHs are crucial enzymes responsible for JH degradation, and are potential targets for selective insecticidal compounds. The cD-NAs of JHEH genes from several insect species have been cloned, including Trichoplusia ni Hübner (Lepidoptera: Noctuidae) (Harris et al 1999), Ctenocephalides felis (Bouche) (Siphonaptera: Pulicidae) (Keiser et al 2002), Bombyx mori L. (Lepidoptera: Bombycidae) (Zhang et al 2005), Apis mellifera L. (Hymenoptera: Apidae) (Mackert et al 2010), Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (Tsubota et al 2010b), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae) (Kamita et al 2013), Manduca sexta L. (Lepidopetra: Sphingidae) (Wojtasek & Prestwich 1996), and Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) (Lü et al 2015). The ability of recombinant JHEHs to degrade JH in vitro has been confirmed in several insect species (Keiser et al 2002;Zhang et al 2005;Tsubota et al 2010b;Kamita et al 2013).…”

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confidence: 99%

Identification and Characterization of Three Juvenile Hormone Genes fromBactrocera dorsalis(Diptera: Tephritidae)

Yang¹,

Xu²,

Shang³

et al. 2016

Florida Entomologist

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Juvenile hormone (JH) plays an important role in regulating growth, development, and reproduction of insects. Three key enzymes, namely, JH esterase (JHE), JH epoxide hydrolase (JHEH), and JH diol kinase (JHDK), are involved in JH degradation. In this study, we identified the full-length cDNAs of the 3 genes BdJHEH2, BdJHEH3, and BdJHDK encoding JHEH and JHDK from the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). We used quantitative real-time polymerase chain reaction to investigate mRNA expression profiles of these 3 genes in various development stages and tissues, and in response to both 20-hydroxyecdysone (20E) and starvation. Both BdJHEH2 and BdJHDK were highly expressed during the larvalpupal transition, whereas BdJHEH3 was mainly expressed in the early 3rd instars. All 3 genes were highly expressed in 7-d-old and 10-d-old adults, but exhibited sex-specific expression patterns. BdJHEH2 was highly expressed in fat body, whereas BdJHEH3 and BdJHDK were most abundant in Malpighian tubules. In response to 20E exposure, the 3 genes were significantly up-regulated at various time points compared with the control. However, the transcript levels of BdJHDK decreased significantly during the initial exposure to 20E. After starvation treatment, expression of BdJHEH2 and BdJHEH3 significantly decreased, whereas BdJHDK was up-regulated. No significant change was observed after feeding resumption. These 3 genes have distinct roles in regulating growth and development of B. dorsalis.

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“…At the wandering stage, JH titer obviously decreased [41], and the expression of JH degradation genes were activated [39]. Here we found that ingestion of JH (Fig 2F), or knockdown of an allatostatin gene ( AS-C ) [41] or either of two JH degradation genes (JH epoxide hydrolase, JHEH1 ; JH diol kinase, JHDK ) [35, 39] to delay JH decrease significantly reduced light avoidance (Fig 2G and S1E-G Fig) and postponed the occurrence of wandering (S2 Table); whereas knockdown of a JH biosynthesis gene (JH acid methyl transferase, JHAMT ) [34] and a JH receptor gene (methoprene-tolerant, Met ) to prematurely reduce JH signal enhanced light avoidance (Fig 2H and S1H, I Fig) and accelerated the onset of wandering (S2 Table). It is clear that JH signal inhibits the premature switch of light preference and the early onset of wandering.…”

Section: Resultsmentioning

confidence: 99%

Hormonal signaling cascades required for phototaxis switch in wanderingLeptinotarsa decemlineatalarvae

Meng

Zhu

et al. 2018

Preprint

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Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle

Cited by 32 publications

References 59 publications

Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

Juvenile Hormone Epoxide Hydrolase: a Promising Target for Hemipteran Pest Management

Identification and Characterization of Three Juvenile Hormone Genes fromBactrocera dorsalis(Diptera: Tephritidae)

Hormonal signaling cascades required for phototaxis switch in wanderingLeptinotarsa decemlineatalarvae

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