Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas' disease described by Carlos Chagas in 1909

Lana, Marta de; Chiari, Cléa de Andrade; Chiari, Égler; Morel, Carlos Médicis; Gonçalves, A. Pedro; Romanha, Álvaro J.

doi:10.1007/s004360050106

Cited by 24 publications

(14 citation statements)

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“…These findings could be related to the reduction of genetic complexity in T. cruzi strains isolated from chronic chagasic patients (Morel et al 1980;Tibayrenc and Ayala 1988;Oliveira et al 1997;Macedo et al 1992). Another explanation should be due to the fact that in the exact moment of parasite isolation from the host, a particular population could predominate over others, since in the T. cruzi life cycle, bloodstream trypomastigotes release does not occur by circadian rhythm (Lana et al 1996). Recently, comparative data analysis of the RAPD and isoenzyme profiles has shown two distinct groups of T. cruzi isolated from chronically infected dogs with Berenice-78 strain after long-term infection (Veloso et al 2005).…”

Section: Discussionmentioning

confidence: 83%

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

et al. 2006

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The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, lambdagt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas' disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.

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Section: Discussionmentioning

confidence: 83%

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

et al. 2006

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“…Although isolated from the same patient, these two strains displayed several biological and molecular differences [19], [31]–[33]. However, because only two alleles were detected for the molecular markers used in their characterization, both strains were initially supposed to be monoclonal [34].…”

Section: Resultsmentioning

confidence: 99%

Unequivocal Identification of Subpopulations in Putative Multiclonal Trypanosoma cruzi Strains by FACs Single Cell Sorting and Genotyping

et al. 2012

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Trypanosoma cruzi, the etiological agent of Chagas disease, is a polymorphic species. Evidence suggests that the majority of the T. cruzi populations isolated from afflicted humans, reservoir animals, or vectors are multiclonal. However, the extent and the complexity of multiclonality remain to be established, since aneuploidy cannot be excluded and current conventional cloning methods cannot identify all the representative clones in an infection. To answer this question, we adapted a methodology originally described for analyzing single spermatozoids, to isolate and study single T. cruzi parasites. Accordingly, the cloning apparatus of a Fluorescence-Activated Cell Sorter (FACS) was used to sort single T. cruzi cells directly into 96-wells microplates. Cells were then genotyped using two polymorphic genomic markers and four microsatellite loci. We validated this methodology by testing four T. cruzi populations: one control artificial mixture composed of two monoclonal populations – Silvio X10 cl1 (TcI) and Esmeraldo cl3 (TcII) – and three naturally occurring strains, one isolated from a vector (A316A R7) and two others derived from the first reported human case of Chagas disease. Using this innovative approach, we were able to successfully describe the whole complexity of these natural strains, revealing their multiclonal status. In addition, our results demonstrate that these T. cruzi populations are formed of more clones than originally expected. The method also permitted estimating of the proportion of each subpopulation of the tested strains. The single-cell genotyping approach allowed analysis of intrapopulation diversity at a level of detail not achieved previously, and may thus improve our comprehension of population structure and dynamics of T. cruzi. Finally, this methodology is capable to settle once and for all controversies on the issue of multiclonality.

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“…He gave a detailed clinical description of the acute phase of the disease and linked the infection with some chronic symptoms of the illness which was remarkable considering that the chronic phase of American trypanosomiasis usually appears decades after the first inoculation with T. cruzi (reviewed in [46]). Interestingly, his first patient Berenice never developed determinate chronic Chagas disease and died at the age 73 years on unrelated causes [47]. However, she was infected with T. cruzi for her whole life as was confirmed by the isolation of parasites when she was 55 and 71 years old [47].…”

Section: Reviewmentioning

confidence: 99%

“…Interestingly, his first patient Berenice never developed determinate chronic Chagas disease and died at the age 73 years on unrelated causes [47]. However, she was infected with T. cruzi for her whole life as was confirmed by the isolation of parasites when she was 55 and 71 years old [47]. In 1912, Chagas reported that he had detected T. cruzi in an armadillo and thus found the first sylvatic reservoir host [48].…”

Section: Reviewmentioning

confidence: 99%

The history of Chagas disease

Steverding

2014

Parasit Vectors

122

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The ancestor of Trypanosome cruzi was probably introduced to South American via bats approximately 7-10 million years ago. When the first humans arrived in the New World, a sylvatic cycle of Chagas disease was then already well established. Paleoparasitological data suggests that human American trypanosomiasis originated in the Andean area when people founded the first settlements in the coastal region of the Atacama Desert. Identification of T. cruzi as the etiological agent and triatome bugs as the transmission vector of Chagas disease occurred within a few years at the beginning of the 20th century. History also teaches us that human activity leading to environmental changes, in particular deforestation, is the main cause for the spread of Chagas disease. Recently, migration of T. cruzi-infected patients has led to a distribution of Chagas disease from Latin America to non-endemic countries in Europe, North America and western Pacific region.

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Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas' disease described by Carlos Chagas in 1909

Cited by 24 publications

References 14 publications

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

Unequivocal Identification of Subpopulations in Putative Multiclonal Trypanosoma cruzi Strains by FACs Single Cell Sorting and Genotyping

The history of Chagas disease

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