1996
DOI: 10.1016/s0022-1759(96)00153-6
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Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils

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Cited by 64 publications
(63 citation statements)
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“…The plasma concentration of NGAL and gelatinase has previously been shown to closely mirror the granulocyte count in healthy adults. 24,25 Thus, the interindividual fluctuation in ficolin-1 concentrations in normal individuals may not within certain limits be dependent on the actual granulocyte count.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The plasma concentration of NGAL and gelatinase has previously been shown to closely mirror the granulocyte count in healthy adults. 24,25 Thus, the interindividual fluctuation in ficolin-1 concentrations in normal individuals may not within certain limits be dependent on the actual granulocyte count.…”
Section: Discussionmentioning
confidence: 99%
“…It has been shown that neutrophils might be the primary source of ficolin-1 in the blood, 21 thus we determined the level of neutrophil gelatinase-associated lipocalin (NGAL) and gelatinase, which reflects the pool of circulating neutrophils. 24,25 No correlation was observed with either NGAL or gelatinase (data not shown).…”
Section: Plasma Levels Of Ficolin-1mentioning
confidence: 90%
“…After 15 min of incubation, cells were pelleted by centrifugation at 200 g for 6 min, the supernatant decanted, and the pellet resuspended to the initial volume in KRP with glucose. The content of myeloperoxidase (azurophil granule marker), lysozyme (azurophil, and specific granule marker), lactoferrin, and NGAL (specific granule markers) and gelatinase (marker for gelatinase granules) was measured in supernatant and pellet by enzyme-linked immunosorbent assays (ELISAs) [18][19][20]. Release of granule marker protein was expressed as content in supernantant in percent of total content (supernatant ϩ pellet).…”
Section: Exocytosis Experimentsmentioning
confidence: 99%
“…Several immunoassays have been developed for the measurement of HNL/NGAL. The assays are based on different formats and include RIA (20), Western blotting (21), ELISA (22,23), Triage device (24), and the Architect platform (16). Several research groups used one of these assays to determine the levels of HNL/NGAL in urine and drew the conclusion that HNL/NGAL is a biomarker of AKI (12,13,16 -18).…”
mentioning
confidence: 99%