Characterization of two complete Isospora mitochondrial genomes from passerine birds: Isospora serinuse in a domestic canary and Isospora manorinae in a yellow-throated miner

Abstract

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe genus term Isospora is now applied specifically to parasites of birds, with the term Cystoisospora preferred for parasites which infect mammals. Isospora is a common parasitic coccidian in birds worldwide, especially in passerine birds, in which it can cause systemic coccidiosis. The complete mitochondrial genome sequences from two recently identified Isospora species; Isospora serinuse in a 3 domestic canary and Isospora manorinae in a yellow-throated miner, were sequenced and compared with those of other closely related coccidian species. The complete mitochondrial genome sequence for Isospora serinuse is 6,260 bp in size and 6,223 bp for Isospora manorinae. The mitochondrial genomes of Isospora serinuse and Isospora manorinae include three protein-coding genes (COI, COIII and CytB), 19 LSU and 14 SSU rDNA fragments, including one newly identified putative LSU fragment in Isospora sp. The arrangement of coding regions in these two Isospora species were identical to that of available Isospora sp. and Eimeria spp. mitochondrial genomes and the start codon usage for protein coding genes was conservative.Phylogenetic analysis of the mt genome of the two Isospora species based on the three coding regions further support that the monophyletic nature of avian Isospora.

The Elusive Mitochondrial Genomes of Apicomplexa: Where Are We Now?

“…Gene arrangement and directions are identical to that of other mitogenomes from Eimeria spp. and others in the family (Yang et al, 2017;Hafeez and Barta, 2019).…”

The Complete Mitochondrial Genome Sequence of Eimeria kongi (Apicomplexa: Coccidia)

“…In the existing literature, the mitochondrial genomes of apicomplexan protozoa, which include species such as Toxoplasma , Cyclospora , Isospora , and Eimeria , are relatively compact, averaging around 6 kilobases (kb) in size [ 23 , 24 , 25 , 26 , 27 , 28 ]. These organisms not only share a similar gene composition but they also exhibit a conserved gene arrangement.…”

The canary Serinus canaria (Passeriformes: Fringillidae) as a new host for Isospora bioccai in Mexico

“…Phylogenetic analysis of the Fringillidae, showed C. sinica cluster separately from congeneric species and form a distinct lineage in the Serinus-Chloris complex ( Zuccon et al, 2012 ). Further morphometric and phylogenetic analysis of coccidia from Fringillidae are need ( Ogedengbe et al, 2016 ; Yang et al, 2017 ). In the canary S. canaria have been identified following Isospora species: I. canaria in USA and Brazil ( Box, 1975 ; Berto et al, 2013 ), I. chloridis in England and Spain ( Anwar, 1966 ; Romero Rodriguez, 1973 ), I. lacazei in England and Spain ( Anwar, 1966 ; Romero Rodriguez, 1973 ), I. serini in USA ( Box, 1975 , 1977 ), and I. serinuse in Australia ( Yang et al, 2015 ).…”