Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants

Kellmann, Jan-Wolfhard; Kleinow, Tatjana; Engelhardt, Kerstin; Philipp, Christina; Wegener, Dorothee; Schell, Jeff; Schreier, Peter

doi:10.1007/bf00020121

Cited by 40 publications

(18 citation statements)

References 43 publications

(46 reference statements)

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“…Moreover, there is no evidence that the constitutively expressed gene in either case contributes to defense. The observation that two members of the peanut class II chitinase gene family, A.h.Chi2;1 and A.h.Chi2;2, are induced upon inoculation with fungal spores in suspension cultured cells, but that only A.h.Chi2;2 responds to exogenous ethylene or SA, suggests that different signaling pathways activate the expression of the two genes (Kellmann et al, 1996). However, it is not known whether the encoded chitinases play similar roles in defense or whether this differential regulation also occurs in planta during infection.…”

Section: Discussionmentioning

confidence: 99%

Tandemly Duplicated Arabidopsis Genes That Encode Polygalacturonase-Inhibiting Proteins Are Regulated Coordinately by Different Signal Transduction Pathways in Response to Fungal Infection

et al. 2002

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Polygalacturonase-inhibiting proteins (PGIPs) are plant proteins that counteract fungal polygalacturonases, which are important virulence factors. Like many other plant defense proteins, PGIPs are encoded by gene families, but the roles of individual genes in these families are poorly understood. Here, we show that in Arabidopsis, two tandemly duplicated PGIP genes are upregulated coordinately in response to Botrytis cinerea infection, but through separate signal transduction pathways. AtPGIP2 expression is mediated by jasmonate and requires COI1 and JAR1 , whereas AtPGIP1 expression is upregulated strongly by oligogalacturonides but is unaffected by salicylic acid, jasmonate, or ethylene. Both AtPGIP1 and AtPGIP2 encode functional inhibitors of polygalacturonase from Botrytis, and their overexpression in Arabidopsis significantly reduces Botrytis disease symptoms. Therefore, gene duplication followed by the divergence of promoter regions may result in different modes of regulation of similar defensive proteins, thereby enhancing the likelihood of defense gene activation during pathogen infection.

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Section: Discussionmentioning

confidence: 99%

Tandemly Duplicated Arabidopsis Genes That Encode Polygalacturonase-Inhibiting Proteins Are Regulated Coordinately by Different Signal Transduction Pathways in Response to Fungal Infection

et al. 2002

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“…Such transgenic peanut plants could defend themselves against pathogens through production of a variety of hydrolytic enzymes and pathogenesis-related proteins (PR proteins) (Vellicce et al, 2006;Jia et al, 2011). Chitinase is one of the proteins of interest in this context, as it is a PR protein that has a role in plant defense responses against fungal pathogens (Kellmann et al, 1996;Liu et al, 2007). Production of chitinase can be induced in plants by inoculation with pathogenic fungi and by exogenous application of salicylic acid (SA) (Kellmann et al, 1996;Qu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Chitinase is one of the proteins of interest in this context, as it is a PR protein that has a role in plant defense responses against fungal pathogens (Kellmann et al, 1996;Liu et al, 2007). Production of chitinase can be induced in plants by inoculation with pathogenic fungi and by exogenous application of salicylic acid (SA) (Kellmann et al, 1996;Qu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional analysis of the chitinase gene promoter in peanut

Chen¹,

Song²,

Yu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT.Chitinase is an important pathogenesis-related protein in plants, and it can accumulate when induced by salicylic acid (SA) or other elicitors. Here, we found that chitinase mRNA levels were 4.5-times greater when peanut seedlings were sprayed with 1.5 mM SA, as compared to water. The upstream promoter sequence of the chitinase gene was cloned by TAIL-PCR and the potential cis-regulatory elements in this promoter were predicted by the cis-element databases PLACE and plantCARE. Elements in the promoter related to SA induction and disease resistance response included AS-1, GT1-motif, GRWAAW, TGTCA, W-box, and WB-box. The full-length promoter (P) and a series of 5'-deleted promoters (P 1 -P 5 ) were cloned and then substituted for the 35S promoter of pCAMBIA1301-xylA, which carries the xylose isomerase gene as the selectable marker and GUS as the reporter gene. Six plant expression vectors (pCAMBIA1301-xylA-P-pCAMBIA1301-xylA-P 5 ) were obtained. The six expression vectors were then transferred into onion epidermal cells and peanut plants by Agrobacterium-mediated transformation. Both the full-length and deleted promoters resulted in GUS staining of the onion epidermis cells when 12711 Characterization of peanut chitinase gene promoter ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 12710-12722 (2015) induced by SA. In onion epidermis cells, GUS enzyme activity was greater after SA induction. In transgenic peanut plants, GUS mRNA levels were greater after SA induction. Consideration of the cis-regulatory elements predicted by PLACE and plantCARE suggested that AS-1, GRWAAW, and W-box are positive regulatory elements in P 2 and P 3 and that GT1-motif and TGTCA are negative regulatory elements between P and P 2 .

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“…Peanut itself contains class II chitinases, which are acti ve against cert ain fungi (Kellm ann et al ., 1996) . Wild species of Arachis may contain chitinases which are candidate gene s for transfer to culti vated peanut for imparting fungal resistanc e. Some microorganisms produce phytotoxic substances which play an important role in causing the disease .…”

Section: Fungal and Bacterial Resistancementioning

confidence: 99%

Regeneration and Genetic Transformation in Peanut: Current Status and Future Prospects

Eapen

2003

Focus on Biotechnology

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Peanut, Arachis hypogaea L. has long been the focus of conventional plant breeding efforts because of its importance as a source of high quality oil and protein. Recent techniques in genetic engineering coupled with developments in regeneration technology can be used for introduction of agronomically useful traits into established cultivars, which will supplement the conventional breeding programmes. Rapid strides have been made in the last two decades to develop a regeneration system for peanut. Highly reproducible regeneration systems through proliferation of axillary buds around a cultured rneristem, de novo shoot organogenesis and somatic embryogenesis are available today as target explants for experiments on transformation. Success has been achieved in the development of transgenic plants using both Agrobacterium-mediated and direct DNA transfer using particle gun bombardment. Several useful genes have already been transferred to peanut using gene transfer techniques . Peanut production is severely limited by a number of diseases and pests and one of the most challenging needs of the day is to improve resistance to Aspergillus species which produce aflatoxins -which are potent carcinogenic metabolites. In addition, introduction of value added traits such as altered protein/oil composition today lies within the realms of biotechnology. Developing peanut plants with genes for abiotic stress or edible vaccines is not far away. The candidate genes which are currently available for transfer to peanut with possible implications in groundnut breeding programme is discussed. As developments in plant biotechnology unfolds, high frequency, disease resistant, drought tolerant and good tasting peanut, which are safe to cat, will continue to be the goals of peanut biotechnologists .

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Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants

Cited by 40 publications

References 43 publications

Tandemly Duplicated Arabidopsis Genes That Encode Polygalacturonase-Inhibiting Proteins Are Regulated Coordinately by Different Signal Transduction Pathways in Response to Fungal Infection

Tandemly Duplicated Arabidopsis Genes That Encode Polygalacturonase-Inhibiting Proteins Are Regulated Coordinately by Different Signal Transduction Pathways in Response to Fungal Infection

Cloning and functional analysis of the chitinase gene promoter in peanut

Regeneration and Genetic Transformation in Peanut: Current Status and Future Prospects

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