Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum

Biosynthesis of fungal terpenoids

“…31,33,[89][90][91] It is worth noting that AaTPS1, a homologue of NfSS, has recently been reported in Alternaria alternata MB-30, and the conguration at position C7 of its cyclization product 68 has been revised by X-ray crystallographic analysis of its derivative. 92 Besides some of the above-mentioned enzymes that use H-4 + to trigger C2-C12 cyclization (path a in Scheme 9), H-4 + can also undergo direct C13 deprotonation to yield Bm1 (70) by BmTS1 from the fungus B. maydis ATCC48331 (path d in Scheme 9). 89 In 2021, Jiang et al identied two sesterterpene cyclases, FoFS and AtAS, from phytopathogenic fungi, which can catalyse the formation of enantiomeric sesterterpenes with a 5/6/7/3/5 ring system.…”

Genomics-driven derivatization of the bioactive fungal sesterterpenoid variecolin: Creation of an unnatural analogue with improved anticancer properties

“…Since the discovery and characterization of the first sesterterpene synthase in 2013 5 , the biosynthetic pathways of several fungal sesterterpenoids have been elucidated 6 , 7 , 8 , 9 . Several new sesterterpenoids have been obtained using a genome mining approach that targets unexploited fungal sesterterpene synthase genes 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 . The characterized fungal sesterterpene synthases were found to be chimeric proteins comprising C-terminal prenyltransferase (PT) and N-terminal terpene cyclase (TC) domains.…”

Two Sesterterpene Synthases from Lentzea atacamensis Demonstrate the Role of Conformational Variability in Terpene Biosynthesis

“…[21,22] More recently AcldAS from Aspergillus calidoustus for asperterpenol A, [23] AuAS from Aspergillus ustus and AcAS from Aspergillus calidoustus for aspergiltriene and aspergildienes AÀ D, [24,25] FoFS from Fusarium oxysporum for fusoxypenes AÀ C and astellatene, [26] AtAS from Aspergillus terreus for preaspterpenacid I, [26] CsSS from Cytospora schulzeri for schultriene, [27] NnNS from Nectria nigrescens for nigtetraene, [27] PstA from Penicillium herquei for penisentene, [28] and AaTPS1 and AaTPS2 from Alternaria alternata for sesteraltererol and preterpestacin I were identified. [29] In contrast, sesterterpene assembly in plants is carried out by two discrete enzymes, a GFPP synthase (GFPPS) and a StTS, that are often genetically clustered. [30][31][32][33] Also in bacteria sesterterpene biosynthesis is catalyzed by two independent GFPPS and StTS enzymes.…”