Characterization of tumour necrosis factor- -related apoptosis-inducing ligand and its receptors in the adult human testis

Grataroli, Renée

doi:10.1093/molehr/gah016

Cited by 34 publications

(37 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…19 In contrast with the rat testis, TRAIL-R1 was also immunodetected in myoid and Sertoli cells in the human testis. 20 Our immunohistochemical data were similar to those in the Grataroli et al study. 19 We found that, TRAIL-R1 was specifically present in meiotic spermatocytes only in the control testis.…”

Section: Discussionsupporting

confidence: 91%

“…There are similar reports, but they do not use testicular rat tissue. Grataroli et al 20 reported the immunolocalization of TRAIL , its receptors and their identification by analyzing proteins and mRNA in human testes. They found that postmeiotic germ cells immuno-expressed both TRAIL and its four receptors, while Leydig cells expressed TRAIL, TRAIL-R2 and TRAIL-R4.…”

Section: Discussionmentioning

confidence: 99%

“…[16][17][18] Immunohistochemical studies indicate that TRAIL, TRAIL-R2 and TRAIL-R4 were detected in Leydig cells, whereas the ligands and all receptors were localized in germ cells. 19,20 The aim of this study is to investigate the possible role of TRAIL pathway activation in the pathogenesis of infertility in an experimental rat model.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

Çelik¹,

Kutlu²,

Tekcan³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

The higher frequency of varicocele in men with infertility has drawn attention and resulted in increased research at the molecular level towards treatments. The aim of this study was to investigate the role of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its receptors in varicocele-induced testicular dysfunction in an experimental rat model. The rats were divided into three groups: control, sham and varicocele. Varicoceles in rats were induced by partial ligation of the left renal vein and left testes. The rats were analyzed 13 weeks after surgery. The degree of DNA fragmentation within cells in the testis was determined using terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assay. Tubule degeneration was evaluated using the Johnsen score. The expression of TRAIL and its receptors was detected by immunohistochemical and Western blotting techniques. The apoptotic index, Johnsen score and the expression of TRAIL and TRAIL receptors were examined. The data are presented as the mean6s.d. and were analyzed using computer software. The Kruskal-Wallis and Dunn's multiple comparison tests were used in the statistical analyses. The germ cell apoptotic index was increased in rats with varicoceles when compared with the sham and control groups (P50.0031). The Johnsen score was significantly decreased in the varicocele group when compared with the sham and control groups (P,0.0001). Immunohistochemical and Western blotting analyses showed that after varicocele induction, the expression of TRAIL-R1 and TRAIL-R4 in germ cells was increased and the expression of TRAIL-R2 was decreased. There are no significant differences among the groups in terms of TRAIL and TRAIL-R3 receptor expression. The results of this study indicate that TRAIL and its receptors may have a potential role in the pathogenesis of varicocele-induced testicular dysfunction.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

Çelik¹,

Kutlu²,

Tekcan³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…It is a key regulator of SCs differentiation and its over-expression can be associated with an impairment of SCs maturation process, reported as a feature of KS testis [15]. The increased cell death in KS testis can also be explained by the observed over-expression of TNFRSF10B, TNFAIP3, CCNG1, BNIP3 and BNIP3L known to regulate natural testes apoptosis and proliferative capacity of LCs [16][17][18]. Among the down-regulated transcripts we found genes that promote cell cycle progression (BRCA1, BTRC and TRIP13).…”

Section: Testis Cell Death In Ks and Deregulation Of Genes Involved Imentioning

confidence: 99%

Deregulation of Sertoli and Leydig cells function in patients with Klinefelter syndrome as evidenced by testis transcriptome analysis

Ferlin¹,

Nicola²,

Garolla³

et al. 2015

EJEA

View full text Add to dashboard Cite

Background: Klinefelter Syndrome (KS) is the most common abnormality of sex chromosomes (47,XXY) and represents the first genetic cause of male infertility. Mechanisms leading to KS testis degeneration are still not completely defined but considered to be mainly the result of germ cells loss. In order to unravel the molecular basis of global testis dysfunction in KS patients, we performed a transcriptome analysis on testis biopsies obtained from 6 azoospermic non-mosaic KS patients and 3 control subjects. Results: The analysis found that, compared to controls, KS patients showed the differential up-and down-expression of 656 and 247 transcripts. The large majority of the deregulated transcripts were expressed by Sertoli cells (SCs) and Leydig cells (LCs). Functional analysis of the deregulated transcripts indicated changes of genes involved in cell death, inflammatory response, lipid metabolism, steroidogenesis, blood-testis-barrier formation and maintenance, as well as spermatogenesis failure.

show abstract

“…Among different tissues, it is noteworthy that TRAIL and its receptors are abundantly expressed in human testis (Grataroli et al 2004). Although TRAIL has been proposed to contribute to the control of the number of spermatogonia (Coureuil et al 2010), the physiopathological role of TRAIL in testis remains to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Soluble TRAIL is present at high concentrations in seminal plasma and promotes spermatozoa survival

Zauli¹,

Celeghini²,

Monasta³

et al. 2014

Reproduction

View full text Add to dashboard Cite

The expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL(TNFSF10)) and of its receptors (TRAILR1, TRAILR2, TRAILR3, and TRAILR4) have been documented in testis, but the presence of soluble TRAIL in seminal fluid, as well as the potential physiopathological role of the TRAIL/TRAILR system in spermatozoa, has not been previously investigated. Male donors (nZ123) among couples presenting for infertility evaluation were consecutively enrolled in this study. The presence of soluble TRAIL was analyzed in seminal samples by ELISA, while the surface expression of TRAIL receptors was investigated by flow cytometry. High levels of soluble TRAIL were detected in seminal plasma (median, 11 621 pg/ml and meanGS.D., 13 371G8367 pg/ml) and flow cytometric analysis revealed a variable expression of TRAIL receptors in the sperm cellular fraction among different subjects. In addition, the effect of physiologically relevant concentrations of recombinant TRAIL was investigated on survival and motility of spermatozoa. Of interest, the in vitro exposure of capacitated spermatozoa to recombinant TRAIL (10 ng/ml) significantly preserved their overall survival. Therefore, the present study demonstrates for the first time the presence of elevated levels of the anti-inflammatory cytokine TRAIL in seminal fluids. Moreover, the demonstration that recombinant TRAIL promotes spermatozoa survival after capacitation suggests potential therapeutic implications.

show abstract

Characterization of tumour necrosis factor- -related apoptosis-inducing ligand and its receptors in the adult human testis

Cited by 34 publications

References 46 publications

Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

Role of TNF-related apoptosis-inducing ligand (TRAIL) in the pathogenesis of varicocele-induced testicular dysfunction

Deregulation of Sertoli and Leydig cells function in patients with Klinefelter syndrome as evidenced by testis transcriptome analysis

Soluble TRAIL is present at high concentrations in seminal plasma and promotes spermatozoa survival

Contact Info

Product

Resources

About