Characterization of Trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human Chagas disease in Santa Catarina State, Brazil

Steindel, Mário; Pacheco, Letícia Kramer; Scholl, Daniele; Soares, Marcos Hirata; Moraes, Milene Höehr de; Eger, Iriane; Kosmann, Cecília; Sincero, Thaís Cristine Marques; Stoco, Patrícia Hermes; Murta, Silvane Maria Fonseca; Carvalho-Pinto, Carlos José de; Grisard, Edmundo C.

doi:10.1016/j.diagmicrobio.2007.07.016

Cited by 143 publications

(109 citation statements)

References 39 publications

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“…The confirmation that they were pure cultures was evidenced by their single 330-bp amplicon derived from the kDNA minicircles 25,27 , and their products from the mini-exon gene non-transcribed spacer 28,29 ( Figure 2), which had 250bp in most isolates and 200bp only in one stock (CT-IOC 541) identified as TcI. The isoenzyme analyses also confirmed the presence of T. cruzi in all isolates (Figure 3), as well as resolved their DTU identification 24,31,32,33 ( Figure 4 and Table 1). …”

Section: Discussionmentioning

confidence: 49%

“…All isolates were also analyzed by multilocus enzyme electrophoresis (MLEE) at four selected loci, which enable the identification of T. cruzi DTUs, their mixtures, T. rangeli, and other trypanosome species 13,24,25,28,[30][31][32][33] , as follows: malate dehydrogenase (MDH, E.C.1.1.1.37), glucose phosphate isomerase (GPI, E.C.5.3.1.9), phosphoglucomutase (PGM, E.C.2.7.5.1), and malic enzyme (ME, E.C.1.1.1.40).…”

Section: Molecular and Biochemical Characterizationmentioning

confidence: 99%

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Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

Oliveira¹,

Santos²,

Galdino³

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Section: Discussionmentioning

confidence: 49%

Section: Molecular and Biochemical Characterizationmentioning

confidence: 99%

Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

Oliveira¹,

Santos²,

Galdino³

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…Our results showed that using two molecular markers was not sufficient for typing of T. cruzi isolates. These limitations were also observed by other authors 32,[60][61][62][63] . However, the use of three genetic markers with different evolutionary rates was able to ensure the correct typing of T. cruzi isolates.…”

Section: Discussionmentioning

confidence: 61%

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Volpato

Sousa

D’Ávila

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. Methods: Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T. cruzi isolates were genotyped using three different genetic markers. Results: Blood culture was positive in 54.9% of all patients, among which 60.3% had the cardiac form of ChD, and 39.1% the indeterminate form of ChD. There were no significant differences in blood culture positivity among patients with cardiac and indeterminate forms. Additionally, patient age and clinical forms did not influence blood culture results. Polymerase chain reaction (PCR) was positive in 98.9% of patients, although comparisons between blood culture and PCR results showed that the two techniques did not agree. Forty-two T. cruzi stocks were isolated, and TcII was detected in 95.2% of isolates. Additionally, one isolate corresponded to TcIII or TcIV, and another corresponded to TcV or TcVI. Conclusions: Blood culture and PCR were both effective for identifying T. cruzi using a single blood sample, and their association did not improve parasite detection. However, we were not able to establish an association between the clinical form of ChD and the genetic profile of the parasite.

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“…Recent studies have shown that infected domestic animals are an important reservoir for sustaining natural transmission in endemic areas [4 • ,5]. Moreover, microepidemics due to ingestion of contaminated juices have been recently reported in Brazil, clearly showing the presence of contaminated insects even in areas where epidemiological control was thought to have been achieved [6]. For years, Chagas disease was considered one of the consequences of poor socioeconomic conditions in Latin America.…”

Section: Human Chagas Disease: An Old Disease Many New Concernsmentioning

confidence: 99%

Current concepts in immunoregulation and pathology of human Chagas disease

Dutra¹,

Gollob

2008

Current Opinion in Infectious Diseases

113

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Purpose of review-Chagas disease is a complex ailment caused by infection with Trypanosoma cruzi. It afflicts millions in Latin America. Years of studies have focused on the development of pathology in Chagas disease and recent studies have helped us understand the cellular mechanisms behind differential clinical evolution of Chagas disease.Recent findings-We discuss recent findings concerning the cellular immune response in human Chagas disease focusing on immunoregulation and the development of pathology. We seek to put several findings into the context of a disease that initially controls an extreme and patent infection, and later progresses to a chronic phase marked by the presence (cardiac and digestive forms), or not (indeterminate form), of associated pathology.Summary-Several theories exist to explain differential clinical evolution of Chagas disease. A coherent understanding of these theories will certainly aid in determining what combination of them approximates the true development of chagasic pathology. For achieving the goal of developing a successful therapy or intervention, it is critical that no theory be excluded at this point, but. Rather, rather that a thoughtful analysis and assimilation of the best components of each system into a central theory that best fits the reality of human Chagas disease is desirable.

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Characterization of Trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human Chagas disease in Santa Catarina State, Brazil

Cited by 143 publications

References 39 publications

Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)

Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease

Current concepts in immunoregulation and pathology of human Chagas disease

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