Characterization of Trypanosoma cruzi from Argentina by electrophoretic zymograms

Montamat, Enrique E.; D'oro, Gloria De Luca; Perret, Beatriz; Rivas, C.

doi:10.1016/0001-706x(91)90005-5

Cited by 22 publications

(17 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the first studies using multilocus enzyme electrophoresis , showed that the more abundant (sub) lineages circulating in Argentina were TcI, TcIId, and TcIIb. [43][44][45] Overall, TcII was more prevalent in the domestic area, whereas TcI and TcII infections were found in sylvatic mammals. 46 A recent work suggests that TcI may be involved in infections of immunosuppressed patients, 41 since this genotype was found in tissues different from blood.…”

Section: Resultsmentioning

confidence: 98%

Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR)

Diez¹,

Lorenz²,

Ortíz³

et al. 2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. We have evaluated blood samples of chronic and congenital Trypanosoma cruzi -infected patients from the city of Reconquista located in the northeast of Argentina where no information was previously obtained about the genotype of infecting parasites. Fourteen samples of congenital and 19 chronical patients were analyzed by hybridization with DNA probes of minicircle hypervariable regions (mHVR). In congenital patients, 50% had single infections with TcIId, 7% single infections with TcIIe, 29% mixed infections with TcIId/e, and 7% had mixed infections with TcIId/b and 7% TcIId/b, respectively. In Chronical patients, 52% had single infections with TcIId, 11% single infections with TcIIe, 26% had mixed infections with TcIId/e, and 11% had non-identified genotypes. With these samples, we evaluated the minicircle lineage-specific polymerase chain reaction assay (MLS-PCR), which involves a nested PCR to HVR minicircle sequences and we found a correlation with hybridization probes of 96.4% for TcIId and 54.8% for TcIIe.

show abstract

Section: Resultsmentioning

confidence: 98%

Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR)

Diez¹,

Lorenz²,

Ortíz³

et al. 2010

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Previous studies showed that lineage TC IId (or its zymodeme equivalent) predominated among human isolates from Santiago del Estero and Chaco provinces and in a large set of human isolates from Argentina and Bolivia (Brenière et al, 2002;Burgos et al, 2007;Diosque et al, 2003;Montamat et al, 1992). TC IId was also frequently identified in human isolates from Paraguay (Chapman et al, 1984), and was the prevalent lineage detected in peripheral blood samples from congenital Chagas disease patients in Argentina (Burgos et al, 2007).…”

Section: Discussionmentioning

confidence: 94%

“…Not working with clones may also have biased our results, since there is evidence that mixed infections with two lineages may only be revealed when clones are genotyped (Montamat et al, 1992, Yeo et al, 2005, 2007 or when parasites are typed directly from fecal samples (Bosseno et al, 1996;Marcet et al, 2006) or from other clinical specimens (Burgos et al, 2007(Burgos et al, , 2008. Resident host individuals not examined for infection or who had disappeared shortly before the surveys may also have contributed to the observed household distribution of lineages in an unknown way.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina

Cardinal

Lauricella

Ceballos

et al. 2008

International Journal for Parasitology

110

108

View full text Add to dashboard Cite

Genetic diversity of Trypanosoma cruzi populations and parasite transmission dynamics have been well documented throughout the Americas, but few studies have been conducted in the Gran Chaco ecoregion, one of the most highly endemic areas for Chagas disease, caused by T. cruzi. In this study we assessed the distribution of T. cruzi lineages (identified by PCR strategies) in Triatoma infestans, domestic dogs, cats, humans and sylvatic mammals from two neighboring rural areas with different histories of transmission and vector control in northern Argentina. Lineage II predominated among the 99 isolates characterized and lineage I among the six isolates obtained from sylvatic mammals. Trypanosoma cruzi lineage IIe predominated in domestic habitats; it was found in 87% of 54 isolates from Tr. infestans, in 82% of 33 isolates from dogs, and in the four cats found infected. Domestic and sylvatic cycles overlapped in the study area in the late 1980s, when intense domestic transmission occurred, and still overlap marginally. The introduction of T. cruzi from sylvatic into domestic habitats is likely to occur very rarely in the current epidemiological context. The household distribution of T. cruzi lineages showed that Tr. infestans, dogs and cats from a given house compound shared the same parasite lineage in most cases. Based on molecular evidence, this result lends further support to the importance of dogs and cats as domestic reservoir hosts of T. cruzi. We believe that in Argentina, this is the first time that lineage IIc has been isolated from naturally-infected domestic dogs and Tr. infestans.

show abstract

“…The clonal structure of T. cruzi, without recombinations, as proposed by Tibayrenc et al (1981Tibayrenc et al ( , 1986, can be seen in the electrophoresis of PGM (enzyme with tri-allelic locus), represented as a heterozygous profile in all the clones and subclones. The strains of T. cruzi are not necessarily homogeneous populations and different clones can be disclosed by studies on their antigenic composition (Brenière et al 1991), virulence (Postan et al 1983), isoenzymic patterns (Goldberg & Pereira 1983, Montamat et al 1992, or kDNA patterns (Morel et al 1980). More recently new approachs have been used with the same objective as the random amplified DNA (RAPD), suggesting the possibility of genetic exchange in T.…”

Section: Discussionmentioning

confidence: 99%

Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

Campos

Andrade

1996

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Characterization of Trypanosoma cruzi from Argentina by electrophoretic zymograms

Cited by 22 publications

References 8 publications

Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR)

Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR)

Molecular epidemiology of domestic and sylvatic Trypanosoma cruzi infection in rural northwestern Argentina

Characterization of subpopulations (clones and subclones) of the 21 SF strain of Trypanosoma cruzi after long lasting maintenance in the laboratory

Contact Info

Product

Resources

About