Characterization of trichuris species from porcupine (Hystrix cristata) at zoological garden of Spain

Rivero, Julia; García‐Sánchez, Ángela María; Callejón, Rocío; Cutillas, Cristina

doi:10.1016/j.actatropica.2021.106276

Cited by 4 publications

(3 citation statements)

References 67 publications

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“…Furthermore, various authors have reported the hypothesis that a complex whipworm species may exist in primates, suggesting that different Trichuris species infect both primates and humans [ 32 , 52 , 53 , 54 , 55 , 56 ]. In addition, our results align with those obtained by Rivero et al [ 57 ], where, based on nuclear and mitochondrial markers as well, the Trichuris sequences isolated from porcupines were distinct from the rest of the analyzed sequences, remaining isolated from the rest of the sequences in the obtained tree. As a result, the results obtained confirmed the sister relationship between clade 1 and clade 2 as identified in the phylogenetic tree.…”

Section: Discussionsupporting

confidence: 91%

New Insights on Tools for Detecting β-Tubulin Polymorphisms in Trichuris trichiura Using rhAmpTM SNP Genotyping

Rivero,

Cutillas,

Callejón

2024

Animals

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Soil-transmitted helminth (STH) infections, commonly treated with benzimidazoles, are linked to resistance through single nucleotide polymorphisms (SNPs) at position 167, 198, or 200 in the β-tubulin isotype 1 gene. The aim of this study was to establish a novel genotyping assay characterized by its rapidity and specificity. This assay was designed to detect the presence of SNPs within the partial β-tubulin gene of Trichuris trichiura. This was achieved through the biallelic discrimination at codons 167, 198, and 200 by employing the competitive binding of two allele-specific forward primers. The specificity and reliability of this assay were subsequently confirmed using Trichuris samples isolated from captive primates. Furthermore, a molecular study was conducted to substantiate the utility of the β-tubulin gene as a molecular marker. The assays showed high sensitivity and specificity when applied to field samples. Nevertheless, none of the SNPs within the β-tubulin gene were detected in any of the adult worms or eggs from the analyzed populations. All specimens consistently displayed an SS genotype. The examination of the β-tubulin gene further validated the established close relationships between the T. trichiura clade and Trichuris suis clade. This reaffirms its utility as a marker for phylogenetic analysis.

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Section: Discussionsupporting

confidence: 91%

New Insights on Tools for Detecting β-Tubulin Polymorphisms in Trichuris trichiura Using rhAmpTM SNP Genotyping

Rivero,

Cutillas,

Callejón

2024

Animals

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“…Adult worms were defrosted and dried at room temperature. The morphological identification of different Trichuris species was carried out according to previous studies ( 15 , 18 , 20 , 50 – 52 ). Nonetheless, morphologically the different species of Trichuris are difficult to differentiate.…”

Section: Methodsmentioning

confidence: 99%

The Use of MALDI-TOF MS as a Diagnostic Tool for Adult Trichuris Species

et al. 2022

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Trichuriasis is considered a neglected tropical disease, being the second most common helminthiasis in humans. Detection of Trichuris in routine diagnosis is usually done by microscopic detection of eggs in fecal samples. Other molecular analyses are more reliable and could be used, but these analyses are not routinely available in clinical microbiology laboratories. The use of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is increasing since the last decades due to its recent evidence as a potential role for reliable identification of microorganisms and a few nematodes. But, for parasites detection, normalized protocols and the acquisition and introduction of new species to the database are required. We carried out a preliminary study confirming the usefulness of MALDI-TOF MS for the rapid and reliable identification of Trichuris suis used as control and the creation of an internal database. To create main spectra profiles (MSPs), the different parts of five whipworms (esophagus and intestine) were used, developing different tests to verify the repeatability and reproducibility of the spectra. Thus, to validate the new internal database, 20 whipworms, separating the esophagus and intestine, were used, of which 100% were accurately identified as T. suis, but could not distinguish between both parts of the worm. Log score values ranged between 1.84 and 2.36, meaning a high-quality identification. The results confirmed that MALDI-TOF MS was able to identify Trichuris species. Additionally, a MALDI-TOF MS profile of T. suis proteome was carried out to develop the first internal database of spectra for the diagnosis of trichuriasis and other Trichuris spp.

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“…With the challenges in morphological identification, molecular genetic markers have aided in Trichuris species identification and have been used in the investigation of genetic variability among different hosts. These include the mitochondrial cytochrome c oxidase subunit I (COI), cytochrome b (cytB), 16S ribosomal RNA (rRNA), complete mitochondrial genomes and nuclear internal transcribed spacer 1 and 2 regions (ITS1 and ITS2) (Callejón et al, 2012(Callejón et al, , 2015bHawash et al, 2015;Rivero et al, 2020;Rivero et al, 2022). Among T. globulosa and T. ovis isolated from sheep and goats, Oliveros et al (2000) revealed that these 2 species are synonymous using the ITS2 region.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic evidence of a possible Trichuris globulosa species complex in Arabian camels from Kuwait

Henedi,

Chan,

Youssef

et al. 2024

Parasitology

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During a 1-year study, Trichuris adults were obtained after necropsy of Arabian camels (Camelus dromedarius) from a slaughterhouse in Kuwait. Morphological and molecular identification was performed to confirm the identity of the Trichuris specimens obtained from C. dromedarius. Fifteen male Trichuris specimens were selected, and molecular identification was performed using mitochondrial cytochrome c oxidase subunit I, 12S ribosomal RNA, 16S ribosomal RNA genes and the nuclear internal transcribed spacer 2 (ITS2) region. Through phylogenetic analysis, 2 distinct groups were obtained using the mitochondrial genes, where group 1 showed a close relationship to Trichuris globulosa while group 2 showed a close relationship to Trichuris ovis, providing molecular evidence of a possible T. globulosa species complex. Additionally, the nuclear ITS2 region did not provide enough resolution to distinguish between the 2 groups of Trichuris specimens. Observation of morphological characters revealed variations in the shape of the male spicule sheath, where specimens present either a globular posteriorly truncated swelling or the absence of posteriorly truncated swelling. Moreover, the variations in male spicule sheath does not corroborate with the results of molecular data, suggesting the limited use of this character for identification of T. globulosa. In conclusion, molecular analysis suggests a possible species complex in T. globulosa, with the mitochondrial genetic markers successfully differentiating between the 2 groups. The limited use of the male spicule sheath as a diagnostic character for identification of T. globulosa is suggested.

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Characterization of trichuris species from porcupine (Hystrix cristata) at zoological garden of Spain

Cited by 4 publications

References 67 publications

New Insights on Tools for Detecting β-Tubulin Polymorphisms in Trichuris trichiura Using rhAmpTM SNP Genotyping

New Insights on Tools for Detecting β-Tubulin Polymorphisms in Trichuris trichiura Using rhAmpTM SNP Genotyping

The Use of MALDI-TOF MS as a Diagnostic Tool for Adult Trichuris Species

Phylogenetic evidence of a possible Trichuris globulosa species complex in Arabian camels from Kuwait

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