Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

Mergler, Stefan; Valtink, Monika; Taetz, Katrin; Sahlmüller, Monika; Fels, Gabriele; Reinach, Peter S.; Engelmann, Katrin; Pleyer, Uwe

doi:10.1016/j.exer.2011.09.021

Cited by 37 publications

(41 citation statements)

References 59 publications

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“…Although a sustained elevation of [Ca 2ϩ ] i is frequently observed in different types of TRPV4-positive cells (13,14,27,28), many TRP channels, including TRPV4, partially desensitize in patchclamp experiments (12,14,30). It has been reported that GSK1016790A can cause a rapid downregulation of TRPV4 within 3 min in TRPV4-transfected cells.…”

Section: Discussionmentioning

confidence: 99%

“…Among TRP channels, vanilloid subtype 4 (TRPV4) is gated by a diverse range of stimuli, such as mechanical stimuli, including shear stress and hypotonic cell swelling (19,20), endogenous arachidonic acid metabolites (38), phorbol esters (14,37), and moderate heat (39). TRPV4 is widely expressed throughout the body, including renal (6,13) and airway (3,18,32) epithelia, skin (16) and esophageal (28) keratinocytes, corneal endothelial and epithelial cells (27,31), pancreatic beta cells (21,25,36), and salivary glands (9,22). Notably, in all these different cell types, the TRPV4 channel has been shown to contribute to the regulation of cytosolic Ca 2ϩ levels.…”

mentioning

confidence: 99%

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TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

Zhang

Catalán

Melvin

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

Zhang

Catalán

Melvin

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…More specifically, TRPV4-gated Ca 2+ entry increases vascular permeability to such an extent that barrier disruption and alveolar flooding may occur [313] . Finally, TRPV4 may enable ECs to sense body temperature, because the threshold for the heat-dependent activation for this channel is set at 25-27 ℃ and it is activated by moderate heat in ECs [4,288,317,318] . The results provided by knocked out mice, however, suggest that the steady state release of NO from endothelium is not governed by such a mechanism.…”

Section: Trpv2mentioning

confidence: 99%

Update on vascular endothelial Ca²⁺signalling: A tale of ion channels, pumps and transporters

Moccia

Berra‐Romani²,

Tanzi³

2012

WJBC

110

192

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A monolayer of endothelial cells (ECs) lines the lumen of blood vessels and forms a multifunctional transducing organ that mediates a plethora of cardiovascular processes. The activation of ECs from as state of quiescence is, therefore, regarded among the early events leading to the onset and progression of potentially lethal diseases, such as hypertension, myocardial infarction, brain stroke, and tumor. Intracellular Ca 2+ signals have long been know to play a central role in the complex network of signaling pathways regulating the endothelial functions. Notably, recent work has outlined how any change in the pattern of expression of endothelial channels, transporters and pumps involved in the modulation of intracellular Ca 2+ levels may dramatically affect whole body homeostasis. Vascular ECs may react to both mechanical and chemical stimuli by generating a variety of intracellular Ca 2+ signals, ranging from brief, localized Ca 2+ pulses to prolonged Ca 2+ oscillations engulfing the whole cytoplasm. The well-defined spatiotemporal profile of the subcellular Ca 2+ signals elicited in ECs by specific extracellular inputs depends on the interaction between Ca 2+ releasing channels, which are located both on the plasma membrane and in a number of intracellular organelles, and Ca 2+ removing systems. The present article aims to summarize both the past and recent literature in the field to provide a clear-cut picture of our current knowledge on the molecular nature and the role played by the components of the Ca 2+ machinery in vascular ECs under both physiological and pathological conditions.

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“…Interestingly, similar observations were made in the immortalized clonal cell populations HCEC-B4G12 and HCEC-H9C1 also under serum-free culture conditions [27]. The osmosensor TRPV4 was also identified in human corneal endothelial cells, because exposure to a hypotonic environment increased the intracellular Ca 2+ concentration and TRP-like whole-cell currents in these cells [28]. Therefore, the commonality in thermosensitive TRP functional expression in the corneal epithelial and endothelial layers suggests that these tissues are well poised to compensate for losses in function that are caused by exposure to similar thermal stresses.…”

Section: Corneal Endotheliummentioning

confidence: 50%

Temperature-Sensitive Transient Receptor Potential Channels in Corneal Tissue Layers and Cells

et al. 2014

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We here provide a brief summary of the characteristics of transient receptor potential channels (TRPs) identified in corneal tissue layers and cells. In general, TRPs are nonselective cation channels which are Ca²⁺ permeable. Most TRPs serve as thermosensitive molecular sensors (thermo-TRPs). Based on their functional importance, the possibilities are described for drug-targeting TRP activity in a clinical setting. TRPs are expressed in various tissues of the eye including both human corneal epithelial and endothelial layers as well as stromal fibroblasts and stromal nerve fibers. TRP vanilloid type 1 (TRPV1) heat receptor, also known as capsaicin receptor, along with TRP melastatin type 8 (TRPM8) cold receptor, which is also known as menthol receptor, are prototypes of the thermo-TRP family. The TRPV1 functional channel is the most investigated TRP channel in these tissues, owing to its contribution to maintaining tissue homeostasis as well as eliciting wound healing responses to injury. Other thermo-TRP family members identified in these tissues are TRPV2, 3 and 4. Finally, there is the TRP ankyrin type 1 (TRPA1) cold receptor. All of these thermo-TRPs can be activated within specific temperature ranges and transduce such inputs into chemical and electrical signals. Although several recent studies have begun to unravel complex roles for thermo-TRPs such as TRPV1 in corneal layers and resident cells, additional studies are needed to further elucidate their roles in health and disease.

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Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

Cited by 37 publications

References 59 publications

TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

Update on vascular endothelial Ca²⁺signalling: A tale of ion channels, pumps and transporters

Temperature-Sensitive Transient Receptor Potential Channels in Corneal Tissue Layers and Cells

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Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells

Cited by 37 publications

References 59 publications

TRPV4 activation in mouse submandibular gland modulates Ca2+influx and salivation

TRPV4 activation in mouse submandibular gland modulates Ca2+influx and salivation

Update on vascular endothelial Ca2+signalling: A tale of ion channels, pumps and transporters

Temperature-Sensitive Transient Receptor Potential Channels in Corneal Tissue Layers and Cells

Contact Info

Product

Resources

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TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

TRPV4 activation in mouse submandibular gland modulates Ca²⁺influx and salivation

Update on vascular endothelial Ca²⁺signalling: A tale of ion channels, pumps and transporters