Characterization of Transcription Factors Following Expression Profiling of Medicago truncatula–Botrytis spp. Interactions

Villegas-Fernández, Ángel M.; Krajinski, Franziska; Schlereth, Armin; Madrid, Eva; Rubiales, Diego

doi:10.1007/s11105-014-0710-8

Cited by 9 publications

(30 citation statements)

References 43 publications

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“…No QTLs or associated molecular markers have been reported so far, although molecular characterization of the resistance is in progress (Villegas et al, 2014).…”

Section: Downloaded By [West Virginia University] At 06:40 20 Novembementioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

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176

132

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“…No QTLs or associated molecular markers have been reported so far, although molecular characterization of the resistance is in progress (Villegas et al, 2014).…”

Section: Downloaded By [West Virginia University] At 06:40 20 Novembementioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

Self Cite

176

132

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“…The stability in expression of these two genes was compared across the tissues (shoots and roots), cultivars (DT51 and MTD720), and treatments (well-watered and drought), and Fbox was finally selected as the reference gene in analysis of RT-qPCR data using 2 −ΔΔCt method (Le et al 2012a). The backgroundcorrected raw fluorescence data were exported from Mastercycler® ep realplex system (Eppendorf, Hamburg, Germany) for estimation of the amplification efficiencies of all primer pairs (26 studied TCS-related and Fbox reference genes) based on linear regression of the exponential section of the product curve using the software LinReg PCR (version 2014.2, Academic Medical Center, Amsterdam, Netherlands, 2012) (Online Resource 1) (De Oliveira et al 2010;Hibbeler et al 2008;Villegas-Fernández et al 2014). Serial dilutions of cDNA concentration (in triplicate) from cDNA pool were also used to verify the primer amplification efficiencies.…”

Section: Plant Growth and Drought Experimentsmentioning

confidence: 99%

Differential Expression of Two-Component System–Related Drought-Responsive Genes in Two Contrasting Drought-Tolerant Soybean Cultivars DT51 and MTD720 Under Well-Watered and Drought Conditions

et al. 2014

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Two-component systems (TCSs) have been shown to participate in plant responses to drought. In this study, results of real-time quantitative PCR (RT-qPCR) of 26 selected dehydration-responsive TCS-related genes in roots and shoots of two Vietnamese soybean cultivars (DT51 and MTD720) with contrasting drought-tolerant phenotypes suggest a positive correlation between the number of droughtinducible TCS genes and their drought-tolerant ability. In addition, expression analyses of the roots and shoots indicated that DT51 and MTD720 had distinct drought-responsive TCS expression profiles, suggesting that expression of TCS-related genes are genotype and tissue dependent. Furthermore, nine TCS genes (GmHK07, 16, GmHP08, GmRR04, 16, 32, 34, GmPRR39, and 44) potentially associated with enhanced drought tolerance were identified. Particularly, GmRR34, showing its higher expression levels under both normal and drought conditions in DT51 roots versus MTD720 roots, might be a potential positive regulator of drought tolerance. On the other hand, GmPRR44 was highly recommended as a potential negative regulator of drought tolerance because it exhibited lower expression levels in both tissues of the drought-tolerant DT51 than in those of the drought-sensitive MTD720 under both stressed and unstressed conditions. These two genes deserve in-depth characterization as promising candidates for development of soybean cultivars with improved drought tolerance by using genetic engineering.

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“…These results agree with similar approaches carried out in response to infection by Uromyces striatus (≈13%) (Madrid et al, 2010 ) and Botrytis spp. (≈20%) (Villegas-Fernández et al, 2014 ). A subset of these genes belong to 25 TF families (Figure 5 , Table S3 ), including AUX/IAA, bHLH, E2F, HD, JUMONJI, MYB, SBP, and zinc finger families (C 2 C 2 , C 2 H 2 , LIM), were specifically regulated in the resistant SA1306 genotype suggesting that they act as major regulators of transcription throughout E. pisi defense responses.…”

Section: Discussionmentioning

confidence: 99%

“…truncatula pathosystem (Foster-Hartnett et al, 2007 ; Samac et al, 2011 ; Curto et al, 2015 ) using different genotypes and microarray platforms, such as Mt16kOLI1, Mt16kOLI1plus, and Affymetrix GeneChip® ( http://www.affymetrix.com ), which have increased the knowledge of mechanisms involved in E. pisi resistance in M. truncatula . Several large-scale TF profiling approaches have employed the M. truncatula qPCR-based platform available (Kakar et al, 2008 ) in various studies (Verdier et al, 2008 ; Gao et al, 2010 ; Madrid et al, 2010 ; Villegas-Fernández et al, 2014 ; Noguero et al, 2015 ). In spite of the progress in characterizing TFs, those involved in the expression of stress-related genes in plants remain undiscovered (Singh et al, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

“…Although DNA microarrays have been shown to be five times less sensitive than qPCR (Czechowski et al, 2004 ), due to its high cost qPCR remains a technique used for low- to middle-scale studies. Several large-scale TF profiling approaches have employed the M. truncatula qPCR-based platform available (Kakar et al, 2008 ) in various studies (Verdier et al, 2008 ; Gao et al, 2010 ; Madrid et al, 2010 ; Villegas-Fernández et al, 2014 ). In this study, we screened the TF transcriptome of M. truncatula for altered expression during E. pisi infection using qPCR.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection

et al. 2015

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Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.

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Characterization of Transcription Factors Following Expression Profiling of Medicago truncatula–Botrytis spp. Interactions

Cited by 9 publications

References 43 publications

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Differential Expression of Two-Component System–Related Drought-Responsive Genes in Two Contrasting Drought-Tolerant Soybean Cultivars DT51 and MTD720 Under Well-Watered and Drought Conditions

Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection

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