Characterization of transcript processing of the gene encoding precerebellin-1

Kavety, Balaji; Morgan, James I.

doi:10.1016/s0169-328x(98)00264-2

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…Informatic analysis of the EEG-1L open reading frame identified this gene as a new member of a family of genes containing a C1q globular domain (10). The family of genes containing a C1q globular domain includes the complement protein C1q itself (13), proteins associated with animal hibernation (14), and precerebellin (15). However, experimental evidence is required to determine whether this and other genetic motifs in the EEG-1 transcripts are functional in the translated protein.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of a Gene Expressed during Terminal Differentiation That Encodes a Novel Inhibitor of Growth

Aerbajinai

Lee

Wojda

et al. 2004

Journal of Biological Chemistry

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We report here the cloning and initial characterization of a novel growth-related gene (EEG-1) that is located on the short arm of chromosome 12. Two spliced transcripts were cloned from human bone marrow and human erythroid progenitor cells: EEG-1L containing a 4350-nucleotide open reading frame encoding a putative protein of 1077 amino acids including a C1q-like globular domain, and an alternatively spliced transcript lacking exon 5 (EEG-1S) encodes a significantly smaller coding region and no C1q-like domain. Quantitative PCR revealed expression of both EEG-1 transcripts in all analyzed tissues. Plasmids encoding green fluorescent protein-tagged genes (GFP-EEG-1) were transfected into Chinese hamster ovary cells for localization and functional assays. In contrast to the diffuse cellular localization of the GFP control, GFP-EEG-1L was detected throughout the cytoplasm and excluded from the nucleus, and GFP-EEG-1S co-localized with aggregated mitochondria. Transfection of both isoforms was associated with significantly increased levels of apoptosis. Stable transfection assays additionally demonstrated decreased growth in those cells expressing EEG-1 at higher levels. Quantitative PCR analyses of mRNA obtained from differentiating erythroid cells from blood donors were performed to determine the transcriptional pattern of EEG-1 during erythropoiesis. EEG-1 expression was highly regulated with increased expression at the stage of differentiation associated with the onset of global nuclear condensation and reduced cell proliferation. We propose that the regulated expression of EEG-1 is involved in the orchestrated regulation of growth that occurs as erythroblasts shift from a highly proliferative state toward their terminal phase of differentiation.

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Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of a Gene Expressed during Terminal Differentiation That Encodes a Novel Inhibitor of Growth

Aerbajinai

Lee

Wojda

et al. 2004

Journal of Biological Chemistry

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“…Similarly, we also found a significant increase of cerebellin (36%, p ϭ 0.016). Cerebellin is a peptide derived from its precursor, which is required for synapse integrity and synaptic plasticity (38,39). DISCUSSION Our present peptidome study explored the effects of chronic nicotine on neuropeptides levels in the DS.…”

Section: Table I Changed Prohormone-derived Peptides and Unchanged Knmentioning

confidence: 99%

Chronic Nicotine Treatment Impacts the Regulation of Opioid and Non-opioid Peptides in the Rat Dorsal Striatum

Petruzziello

Falasca

Andrén

et al. 2013

Molecular & Cellular Proteomics

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The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum, an important brain region implicated in the preoccupation/ craving phase of drug dependence. We used extensive LC-FT-MS/MS analyses for neuropeptide identification and LC-FT-MS in conjunction with stable isotope addition for relative quantification. The treatment with chronic nicotine for 3 months led to moderate changes in the levels of endogenous dorsal striatum peptides. Five enkephalin opioid peptides were up-regulated, although no change was observed for dynorphin peptides. Specially, nicotine altered levels of nine non-opioid peptides derived from precursors, including somatostatin and cerebellin, which potentially modulate neurotransmitter release and energy metabolism. This broad but selective impact on the multiple peptidergic systems suggests that apart from the opioid peptides, several other peptidergic systems are involved in the preoccupation/craving phase of drug dependence. Our finding permits future evaluation of the neurochemical circuits modulated by chronic nicotine exposure and provides a number of novel molecules that could serve as potential therapeutic targets for treating drug dependence. Molecular & Cellular

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“…Interestingly, one of the genes in cluster 2, the precerebellin-1 gene (gene 5), is known to be strongly expressed in the cerebellum (Kavety and Morgan 1998). A graphic representation of the spatial expression for precerebellin-1, in both the normal and PD brains, is shown in Figure 4D.…”

Section: Spatial Gene Expression Patternsmentioning

confidence: 99%

Multiplex Three-Dimensional Brain Gene Expression Mapping in a Mouse Model of Parkinson's Disease

Brown

Ossadtchi

et al. 2002

Genome Res.

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To facilitate high-throughput 3D imaging of brain gene expression, a new method called voxelation has been developed. Spatially registered voxels (cubes) are analyzed, resulting in multiple volumetric maps of gene expression analogous to the images reconstructed in biomedical imaging systems. Using microarrays, 40 voxel images for 9000 genes were acquired from brains of both normal mice and mice in which a pharmacological model of Parkinson's disease (PD) had been induced by methamphetamine. Quality-control analyses established the reproducibility of the voxelation procedure. The investigation revealed a common network of coregulated genes shared between the normal and PD brain, and allowed identification of putative control regions responsible for these networks. In addition, genes involved in cell/cell interactions were found to be prominently regulated in the PD brains. Finally, singular value decomposition (SVD), a mathematical method used to provide parsimonious explanations of complex data sets, identified gene vectors and their corresponding images that distinguished between normal and PD brain structures, most pertinently the striatum.

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Characterization of transcript processing of the gene encoding precerebellin-1

Cited by 10 publications

References 24 publications

Cloning and Characterization of a Gene Expressed during Terminal Differentiation That Encodes a Novel Inhibitor of Growth

Cloning and Characterization of a Gene Expressed during Terminal Differentiation That Encodes a Novel Inhibitor of Growth

Chronic Nicotine Treatment Impacts the Regulation of Opioid and Non-opioid Peptides in the Rat Dorsal Striatum

Multiplex Three-Dimensional Brain Gene Expression Mapping in a Mouse Model of Parkinson's Disease

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