Characterization of Trametes versicolor laccase-catalyzed degradation of estrogenic pollutants: Substrate limitation and product identification

Beck, Samuel J.; Berry, Ellie; Duke, Sara E.; Milliken, Alexander S.; Patterson, H.T.; Prewett, Deborah L.; Rae, T.; Sridhar, Varsha; Wendland, Nils; Gregory, Brian W.; Johnson, Corey M.

doi:10.1016/j.ibiod.2017.11.020

Cited by 60 publications

(26 citation statements)

References 44 publications

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“…S6 †). [41][42][43] Fig. 6A shows that immobilized laccases exhibited higher BPA removal capacity with respect to free laccases that 80.2% and 60.4% of BPA were eliminated at the beginning 6 h, respectively.…”

Section: Structural Analysis Of Free and Immobilized Laccasesmentioning

confidence: 97%

Laccase-immobilized tannic acid-mediated surface modification of halloysite nanotubes for efficient bisphenol-A degradation

Zhang

Tang

et al. 2019

RSC Adv.

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Section: Structural Analysis Of Free and Immobilized Laccasesmentioning

confidence: 97%

Laccase-immobilized tannic acid-mediated surface modification of halloysite nanotubes for efficient bisphenol-A degradation

Zhang

Tang

et al. 2019

RSC Adv.

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“…Some authors attribute to the substrate redox potential a more important role in reactivity than interactions with enzyme active site. 47 When different binding modes were predicted, a relevant role of D 206 and H 458 (T. versicolor and T. maxima laccase numbering) for substrate recognition was identified. 48 These results differ from our data for the only common compound between both studies (2,6-DMP).…”

Section: Discussionmentioning

confidence: 99%

Modeling and experimental validation of covalent immobilization of Trametes maxima laccase on glyoxyl and MANA‐Sepharose CL 4B supports, for the use in bioconversion of residual colorants

Cutiño-Avila

Sánchez-López

Cárdenas-Moreno

et al. 2021

Biotech and App Biochem

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Our novel strategy for the rational design of immobilized derivatives (RDID) is directed to predict the behavior of the protein immobilized derivative before its synthesis, by the usage of mathematic algorithms and bioinformatics tools. However, this approach needs to be validated for each target enzyme. The objective of this work was to validate the RDID strategy for covalent immobilization of the enzyme laccase from Trametes maxima MUCL 44155 on glyoxyl‐ and monoaminoethyl‐N‐aminoethyl (MANA)‐Sepharose CL 4B supports. Protein surface clusters, more probable configurations of the protein–supports systems at immobilization pHs, immobilized enzyme activity, and protein load were predicted by RDID1.0 software. Afterward, immobilization was performed and predictions were experimentally confirmed. As a result, the laccase‐MANA‐Sepharose CL 4B immobilized derivative is better than laccase‐glyoxyl‐Sepharose CL 4B in predicted immobilized derivative activity (63.6% vs. 29.5%). Activity prediction was confirmed by an experimentally expressed enzymatic activity of 68%, using 2,6‐dimethoxyphenol as substrate. Experimental maximum protein load matches the estimated value (11.2 ± 1.3 vs. 12.1 protein mg/support mL). The laccase‐MANA‐Sepharose CL 4B biocatalyst has a high specificity for the acid blue 62 colorant. The results obtained in this work suggest the possibility of using this biocatalyst for wastewater treatment.

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“…The Lac activity was confirmed when an orange–yellow staining zone appeared corresponding to the oxidation of DMP to 2,2′,6,6′‐tetramethoxydibenzo‐1,1′‐diquinone (Beck et al . 2018). MnP activity was observed as a brown staining zone due to MnO 2 precipitation (Perez and Jeffries 1992).…”

Section: Methodsmentioning

confidence: 99%

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

et al. 2021

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Aims To evaluate the mycoremediation of polychlorinated biphenyls (PCBs) by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023. Methods and Results PCBs tolerance, removal capacity, toxicity reduction and ligninolytic enzyme expression were assessed when growing single culture and binary consortium of fungus in 217 mg l−1 of a technical mixture of Aroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance and variation in ligninolytic enzyme secretion were observed in PCB‐amended solid media. Pleurotus pulmonarius LBM 105 mono‐culture was able to remove up to 95·4% of PCBs, whereas binary consortium and T. sanguinea LBM 023 could biodegrade about 55% after 24 days. Significant detoxification levels were detected in all treatments by biosorption mechanism. Conclusions Pleurotus pulmonarius LBM 105 in single culture had the best performance regarding PCBs biodegradation and toxicity reduction. Ligninolytic enzyme secretion changed in co‐culture. Significance and Impact of the Study The evaluation of PCBs bioremediation effectiveness of basidiomycetes consortium in terms of PCB removal, toxicity and ligninolytic enzyme production to unravel the differences between using individual cultures or consortium has not been reported. The results from this study enable the selection of P. pulmonarius LBM 105 mono‐culture to bioremediate PCBs as it showed higher efficiency compared to binary consortium with T. sanguinea LBM 023 for potential decontamination of PCB‐contaminated transformer oil.

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Characterization of Trametes versicolor laccase-catalyzed degradation of estrogenic pollutants: Substrate limitation and product identification

Cited by 60 publications

References 44 publications

Laccase-immobilized tannic acid-mediated surface modification of halloysite nanotubes for efficient bisphenol-A degradation

Laccase-immobilized tannic acid-mediated surface modification of halloysite nanotubes for efficient bisphenol-A degradation

Modeling and experimental validation of covalent immobilization of Trametes maxima laccase on glyoxyl and MANA‐Sepharose CL 4B supports, for the use in bioconversion of residual colorants

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

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