Characterization of tomato zonate spot virus, a new tospovirus in China

Dong, Jiahong; Cheng, Xiaofei; Yin, Yueyan; Qi, Fang; Ding, Ming; Li, Tingting; Zhang, Lizhen; Su, Xiaoxia; McBeath, Jenifer Huang; Zhang, Zhongkai

doi:10.1007/s00705-008-0054-5

Cited by 75 publications

(72 citation statements)

References 44 publications

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“…Another tospovirus species, Melon yellow spot virus (MYSV), was originally identified as a distinct serotype (Cortez et al 2001;Kato et al 2000); however, in a subsequent study by Chen et al (2010), serological relationships based on NP between MYSV and WSMoV were demonstrated. More recently, a few additional serologicallydistinct tospovirus species have also been identified (Bag et al 2010;Dong et al 2008;Hassani-Mehraban et al 2005Seepiban et al 2011).…”

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confidence: 97%

Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype

et al. 2012

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Soybean vein necrosis-associated virus (SVNaV) is a newly isolated tospovirus from fieldgrown soybeans in the United States. Polyclonal antibodies generated against the recombinant Escherichia coli-expressed nucleocapsid protein (NP) of the virus, reacted specifically with SVNaV and exhibited low, if any, cross-reactivity with other species within the genus Tospovirus. The serological results are in agreement with low sequence homology of the SVNaV-NP gene when compared with those of other tospovirus species, some of which are capable of infecting soybean naturally. Phylogenetic analysis utilizing NP sequences from several SVNaV isolates collected from different geographical regions and various soybean genotypes over 4 years showed close relationships, but distinct from the representatives of all the established serogroups or distinct serotypes within the genus of Tospovirus. All SVNaV isolates examined reacted strongly with the generated polyclonal antibodies. Collectively, our serological analyses suggest that SVNaV represents a new and distinct soybean-infecting tospovirus serotype. Furthermore, our data demonstrate that antiserum against NP has the potential to serve as a reliable probe for SVNaV detection in field-grown soybeans.

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confidence: 97%

Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype

et al. 2012

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“…Currently, besides the typespecies Tomato spotted wilt virus, there are at least a dozen of other recognized species in this genus (Hassani-Mehraban et al 2005;Jones 2005;Lin et al 2005;Whitfield et al 2005;Ciuffo et al 2008;Dong et al 2008;Ciuffo et al 2009;Hassani-Mehraban et al 2010).…”

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confidence: 99%

Search in Solanum (section Lycopersicon) germplasm for sources of broad-spectrum resistance to four Tospovirus species

et al. 2011

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The genus Tospovirus was considered as monotypic with Tomato spotted wilt virus (TSWV) being the only assigned species. However, extensive studies with worldwide isolates revealed that this genus comprises a number of species with distinct virulence profiles. The Neotropical South America is one center of Tospovirus diversity with many endemic species. Groundnut ringspot virus (GRSV), TSWV, Tomato chlorotic spot virus (TCSV), and Chrysanthemum stem necrosis virus (CSNV) are the predominant tomato-infecting species in Brazil. Sources of resistance were found in Solanum (section Lycopersicon) mainly against TSWV isolates from distinct continents, but there is an overall lack of information about resistance to other viral species. One-hundred and five Solanum (section Lycopersicon: Solanaceae) accessions were initially evaluated for their reaction against a GRSV isolate by analysis of symptom expression and systemic virus accumulation using DAS-ELISA. A subgroup comprising the most resistant accessions was re-evaluated in a second assay with TSWV, TCSV, and GRSV isolates and in a third assay with a CSNV isolate. Seven S. peruvianum accessions displayed a broad-spectrum resistance to all viral species with all plants being free of symptoms and systemic infection. Sources of resistance were also found in tomato cultivars with the Sw-5 gene and also in accessions of S. pimpinellifolium, S. chilense, S. arcanum, S. habrochaites, S. corneliomuelleri, and S. lycopersicum. The introgression/incorporation of these genetic factors into cultivated tomato varieties might allow the development of genetic materials with broad-spectrum resistance, as well as with improved levels of phenotypic expression.

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“…Whereas TSWV causes infections in tomato all over the world, in South America tomato is also infected by the tospoviruses Chrysanthemum stem necrosis virus (CSNV), Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) (Bezzera et al 1999;de Ávila et al 1993b). In Australia and South-East Asia, tospovirus infections of tomato other than TSWV, include Capsicum chlorosis virus (CaCV) and Watermelon silver mottle virus (WSMoV) (McMichael et al 2002;Chiemsombat et al 2008), in Iran Tomato yellow ring virus (TYRV) (HassaniMehraban et al 2005) and in China Tomato zonate spot virus (TZSV) (Dong et al 2008). Most of these tospoviruses also infect pepper.…”

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Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand

Hassani-Mehraban

Cheewachaiwit²,

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et al. 2011

Eur J Plant Pathol

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Two tospovirus isolates collected from tomato and bell pepper in Thailand were studied. The isolates induced severe necrotic mottling and/or necrotic spots and rings on the leaves and fruits of the respective plants as confirmed by back-inoculation. A polyclonal antiserum raised against its nucleocapsid (N) protein reacted only with an extract from plants infected with the homologous virus. Analysis of the nucleocapsid (N) gene sequence and its deduced amino acid sequence (Mw ∼31 kDa) showed 99% amino acid sequence homology with that of Tomato necrotic ring virus (TNRV). The nucleotide sequence of the 5 untranslated region and intergenic region flanking the N gene revealed typical features of the S RNA segment of tospoviruses. Mechanical inoculation of the virus on some plant species showed that most of the tested solanaceous species were susceptible to this virus. The biological, serological and molecular data presented here indicate that both isolates are identical to TNRV, a recently described tospovirus species in Thailand.

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Characterization of tomato zonate spot virus, a new tospovirus in China

Cited by 75 publications

References 44 publications

Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype

Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype

Search in Solanum (section Lycopersicon) germplasm for sources of broad-spectrum resistance to four Tospovirus species

Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand

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