Characterization of tomato leaf curl purple vein virus, a new monopartite New World begomovirus infecting tomato in Northeast Brazil

Macedo, M. A.; Albuquerque, Leonardo Cunha de; Maliano, Minor R.; Souza, J. O.; Rojas, María R.; Inoue‐Nagata, Alice K.; Gilbertson, R. L.

doi:10.1007/s00705-017-3662-0

Cited by 38 publications

(28 citation statements)

References 29 publications

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“…species have now also emerged) (10)(11)(12). A begomovirus infection starts when whiteflies feed on phloem sap of a noninfected plant, during which they transmit virions in the phloem-associated cells.…”

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confidence: 99%

A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein

Maio

Arroyo-Mateos

Bobay

et al. 2019

J Virol

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Geminiviruses are single-stranded DNA (ssDNA) viruses that infect a wide range of plants. To promote viral replication, geminiviruses manipulate the host cell cycle. The viral protein Rep is essential to reprogram the cell cycle and then initiate viral DNA replication by interacting with a plethora of nuclear host factors. Even though many protein domains of Rep have been characterized, little is known about its nuclear targeting. Here, we show that one conserved lysine in the N-terminal part of Rep is pivotal for nuclear localization of the Rep protein from Tomato yellow leaf curl virus (TYLCV), with two other lysines also contributing to its nuclear import. Previous work had identified that these residues are essential for Rep from Tomato golden mosaic virus (TGMV) to interact with the E2 SUMO-conjugating enzyme (SCE1). We here show that mutating these lysines leads to nuclear exclusion of TYLCV Rep without compromising its interaction with SCE1. Moreover, the ability of TYLCV Rep to promote viral DNA replication also depends on this highly conserved lysine independently of its role in nuclear import of Rep. Our data thus reveal that this lysine potentially has a broad role in geminivirus replication, but its role in nuclear import and SCE1 binding differs depending on the Rep protein examined. IMPORTANCE Nuclear activity of the replication initiator protein (Rep) of geminiviruses is essential for viral replication. We now define that one highly conserved lysine is important for nuclear import of Rep from three different begomoviruses. To our knowledge, this is the first time that nuclear localization has been mapped for any geminiviral Rep protein. Our data add another key function to this lysine residue, besides its roles in viral DNA replication and interaction with host factors, such as the SUMO E2-conjugating enzyme.

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confidence: 99%

A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein

Maio

Arroyo-Mateos

Bobay

et al. 2019

J Virol

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“…Although it could be suggested that there are no begomoviruses capable of infecting cassava in the Americas, the high diversity of begomoviruses reported in a broad range of cultivated and non-cultivated plants in several botanical families, including the Euphorbiaceae, make this highly unlikely (33,(81)(82)(83)(84)(85)(86)(87)(88)(89). Thus, the absence of a competent vector able to colonize cassava and transfer begomoviruses from wild plants to cassava, as previously suggested (47), seems to be a more plausible hypothesis to explain the lack of begomovirus epidemics in this crop.…”

Section: Discussionmentioning

confidence: 99%

Assessing whitefly diversity to infer about begomovirus dynamics in cassava in Brazil

Xavier

Nogueira

Bello

et al. 2020

Preprint

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Plant virus ecology is strongly dependent on that of its vector. The necessity of a competent vector for transmission is a primary ecological factor driving the host range expansion of plant arthropod-borne viruses, with vectors playing an essential role in promoting disease emergence. Cassava begomoviruses severely constrain cassava production in Africa. Curiously, begomoviruses have never been reported in cassava in South America, the center of origin for this crop. It has been hypothesized that the absence of a competent begomoviruses vector that efficiently colonizes cassava is the reason why begomoviruses have not emerged in South America. To test this hypothesis, we performed a country-wide whitefly diversity study in cassava in Brazil. Adults and/or nymphs of whiteflies were collected from sixty-six cassava fields across twelve states representing the main agroecological zones of the country. A total of 1,385 individuals were genotyped based on partial mitochondrial cytochrome oxidase I (mtCOI) sequences. A high species richness was observed, with five previously described species and two putative new ones. The most prevalent species were Tetraleurodes acaciae and Bemisia tuberculata, representing over 75% of the analyzed individuals. Although we detected, for the first time, the presence of Bemisia tabaci Middle East-Asia Minor 1 (BtMEAM1) colonizing cassava in Brazil, it was not prevalent. The species composition varied across regions, with fields in the Northeast region showing a higher diversity. These results expand our knowledge of whitefly diversity in cassava and support the hypothesis that begomovirus epidemics have not occurred in cassava in Brazil due to the absence of competent vector populations. However, they indicate an ongoing adaptation process of BtMEAM1 to cassava, increasing the likelihood of begomovirus emergence in this crop in the near future.

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“…Brazil is considered the largest centre of begomovirus diversity, and it has its own indigenous species. Since the introduction of MEAM1 in the mid‐1990s, more than 17 new species have been reported infecting tomatoes and other solanaceous crops in Brazil (Inoue‐Nagata et al , ; Macedo et al , ; Quadros et al , ). The strongest hypothesis is that some of these species infected weeds and wild hosts before being inoculated by MEAM1 (García‐Arenal and Zerbini, ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies

et al. 2020

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Tomato severe rugose virus (ToSRV) is the most important begomovirus transmitted and spread by the whitefly Bemisia tabaci in tomato crops in Brazil. Cultural practices are being adopted, along with insecticides, for controlling this virus. However, little is known about the importance of weeds in the pathosystem, which can contribute to the failure of these practices. This work aimed to evaluate the role of Datura stramonium and Nicandra physaloides as alternative hosts of ToSRV and verify the viral influence on the biological performance of Bemisia tabaci Middle East‐Asia Minor 1 (MEAM1) and Mediterranean (MED) cryptic species. N. physaloides was a better alternative host for ToSRV when combined with MEAM1 whiteflies, while D. stramonium was mostly a good host for whitefly reproduction. Viral infection improved MEAM1 performance on both host plants but affected MED performance negatively. These data suggest that both weeds can be of some importance for the pathosystem, and their control should be included in management programmes.

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Characterization of tomato leaf curl purple vein virus, a new monopartite New World begomovirus infecting tomato in Northeast Brazil

Cited by 38 publications

References 29 publications

A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein

A Lysine Residue Essential for Geminivirus Replication Also Controls Nuclear Localization of the Tomato Yellow Leaf Curl Virus Rep Protein

Assessing whitefly diversity to infer about begomovirus dynamics in cassava in Brazil

Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies

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