Characterization of Tn
 916
 S, a Tn
 916
 -Like Element Containing the Tetracycline Resistance Determinant
 tet
 (S)

Lancaster, Holli; Roberts, Adam P.; Bedi, Raman; Wilson, Michael; Mullany, Peter

doi:10.1128/jb.186.13.4395-4398.2004

Cited by 51 publications

(34 citation statements)

References 15 publications

(11 reference statements)

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“…This gene has been initially described on a conjugative plasmid of a multiresistant Listeria monocytogenes strain (18) and subsequently on a plasmid of Lactococcus lactis (51) and in the chromosome of Enterococcus faecalis (19). More recently, this gene has been detected in isolates of Streptococcus pyogenes (29), Streptococcus intermedius (32), and S. dysgalactiae subsp. equisimilis (34), suggesting a lateral gene transfer of this tetracycline resistance determinant.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of S treptococcus agalactiae , S. dysgalactiae subsp. dysgalactiae , and S. uberis

Haenni¹,

Saras²,

Bertin

et al. 2010

Appl Environ Microbiol

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Bovine isolates of Streptococcus agalactiae (n ‫؍‬ 76), Streptococcus dysgalactiae subsp. dysgalactiae (n ‫؍‬ 32), and Streptococcus uberis (n ‫؍‬ 101) were analyzed for the presence of different integrative and conjugative elements (ICEs) and their association with macrolide, lincosamide, and tetracycline resistance. The diversity of the isolates included in this study was demonstrated by multilocus sequence typing for S. agalactiae and pulsedfield gel electrophoresis for S. dysgalactiae and S. uberis. Most of the erythromycin-resistant strains carry an ermB gene. Five strains of S. uberis that are resistant to lincomycin but susceptible to erythromycin carry the lin(B) gene, and one has both linB and lnuD genes. In contrast to S. uberis, most of the S. agalactiae and S. dysgalactiae tetracycline-resistant isolates carry a tet(M) gene. A tet(S) gene was also detected in the three species. A Tn916-related element was detected in 30 to 50% of the tetracycline-resistant strains in the three species. Tetracycline resistance was successfully transferred by conjugation to an S. agalactiae strain. Most of the isolates carry an ICE integrated in the rplL gene. In addition, half of the S. agalactiae isolates have an ICE integrated in a tRNA lysine (tRNA Lys ) gene. Such an element is also present in 20% of the isolates of S. dysgalactiae and S. uberis. A circular form of these ICEs was detected in all of the isolates tested, indicating that these genetic elements are mobile. These ICEs could thus also be a vehicle for horizontal gene transfer between streptococci of animal and/or human origin.

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Section: Discussionmentioning

confidence: 99%

Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of S treptococcus agalactiae , S. dysgalactiae subsp. dysgalactiae , and S. uberis

Haenni¹,

Saras²,

Bertin

et al. 2010

Appl Environ Microbiol

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“…PCR primers were designed to amplify the entire gene. The sequence of this PCR product (accession number DQ295784) revealed a tet(S) gene identical to the tet(S) gene from L. monocytogenes, 99% similar to tet(S) from L. lactis plasmid pK214 (accession number X92946) (19), and 98% similar to tet(S) from the recently discovered Tn916S element (accession number AY534326) (15). The PCR product of the internal fragment of the tet(S) gene from E. faecium 664.1H1 was used as a probe in Southern hybridization of E. faecium 664.1H1 genomic and plasmid DNA restricted with HindIII.…”

Section: The Tetracycline Resistance Gene Tet(s) Is Present On the Chmentioning

confidence: 96%

Characterization of the Ends and Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 664.1H1

et al. 2006

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Enterococcus faecium 664.1H1 is multiply antibiotic resistant and mercury resistant. In this study, the genetic support for the tetracycline resistance of E. faecium 664.1H1 was characterized. The tet(S) gene is responsible for tetracycline resistance, and this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon. The element is transferable to Enterococcus faecalis, where it integrates into a specific site. The element was designated EfcTn1. The integrase of EfcTn1 is related to the integrase proteins found on staphylococcal pathogenicity islands. We show that the transposon is flanked by an 18-bp direct repeat, a copy of which is also present at the target site and at the joint of a circular form, and we propose a mechanism of insertion and excision.

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“…The tet(L), tet(M), tet(U), and tet(S) genes were detected in each E. faecium isolate except isolate 5750, which lacked the tet(S) gene. Because tet(S) is reported infrequently (26) and tet(U) has not previously been reported for S. aureus, the DNA sequences of these PCR products were determined and compared with the relevant gene sequences in GenBank [accession numbers AY534326 for tet(S) and EFU01917 for tet(U)]. The percentage of nucleotide identity was 99% for the tet(U) genes and 100% for tet(S) genes compared with the GenBank gene sequences (data not shown).…”

Section: Pcr Amplifications For Tet(k) Tet(l) Tet(m) and Tet(o)mentioning

confidence: 99%

High-Level Vancomycin-Resistant Staphylococcus aureus Isolates Associated with a Polymicrobial Biofilm

Weigel

Donlan

Shin

et al. 2007

Antimicrob Agents Chemother

217

142

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Glycopeptides such as vancomycin are the treatment of choice for infections due to methicillin-resistant Staphylococcus aureus. This study describes the identification of high-level vancomycin-resistant S. aureus (VRSA) isolates in a polymicrobial biofilm within an indwelling nephrostomy tube in a patient in New York. S. aureus, Enterococcus faecalis, Enterococcus faecium, Micrococcus species, Morganella morganii, and Pseudomonas aeruginosa were isolated from the biofilm. For VRSA isolates, vancomycin MICs ranged from 32 to >128 g/ml. VRSA isolates were also resistant to aminoglycosides, fluoroquinolones, macrolides, penicillin, and tetracycline but remained susceptible to chloramphenicol, linezolid, rifampin, and trimethoprim-sulfamethoxazole. The vanA gene was localized to a plasmid of ϳ100 kb in VRSA and E. faecium isolates from the biofilm. Plasmid analysis revealed that the VRSA isolate acquired the 100-kb E. faecium plasmid, which was then maintained without integration into the MRSA plasmid. The tetracycline resistance genes tet(U) and tet(S), not previously detected in S. aureus isolates, were identified in the VRSA isolates. Additional resistance elements in the VRSA isolate included a multiresistance gene cluster, ermB-aadE-sat4-aphA-3, msrA (macrolide efflux), and the bifunctional aminoglycoside resistance gene aac(6)-aph(2؆)-Ia. Multiple combinations of resistance genes among the various isolates of staphylococci and enterococci, including vanA, tet(S), and tet(U), illustrate the dynamic nature of gene acquisition and loss within and between bacterial species throughout the course of infection. The potential for interspecies transfer of antimicrobial resistance genes, including resistance to vancomycin, may be enhanced by the microenvironment of a biofilm.

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Characterization of Tn 916 S, a Tn 916 -Like Element Containing the Tetracycline Resistance Determinant tet (S)

Cited by 51 publications

References 15 publications

Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of S treptococcus agalactiae , S. dysgalactiae subsp. dysgalactiae , and S. uberis

Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of S treptococcus agalactiae , S. dysgalactiae subsp. dysgalactiae , and S. uberis

Characterization of the Ends and Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 664.1H1

High-Level Vancomycin-Resistant Staphylococcus aureus Isolates Associated with a Polymicrobial Biofilm

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