Characterization of thyroxine-albumin binding using high-performance affinity chromatography II. Comparison of the binding of thyroxine, triiodothyronines and related compounds at the warfarin and indole sites of human serum albumin

Studies of phenytoin binding to human serum albumin by high-performance affinity chromatography

“…Eq. (4) has been shown to be valuable in studying compounds with multiple binding sites on HSA by using these agents as the competing agent and site-specific probe as the injected solutes [24,25]. This approach will also be used in this report for examining the binding of phenytoin at each of the major and minor binding sites of HSA.…”

Structural basis of albumin–thyroxine interactions and familial dysalbuminemic hyperthyroxinemia

“…It is a monomeric protein containing three homologous domains (I-III), each divided into two subdomains (A and B). The number and locations of binding sites for T 4 are not well defined (5,6), although a consensus of most recent studies (6)(7)(8)(9)(10)(11) indicates that there are likely to be high-affinity T 4 -binding sites in subdomains IIA and IIIA. These subdomains contain the two primary drug-binding sites on the protein (12,13) and also bind fatty acids (refs.…”

Affinity Chromatography as a Tool for Quantification of Interactions Between Drug Molecules and Their Protein Targets

“…If not, the amount should be decreased until the retention time remains constant and/or increase the volume of the column. Also number of injections at different flow rates should be done to confirm that the processes of association/dissociation are fast enough (compared to how much time analyte spends in the column) to create inside the column local state of a binding equilibrium (Loun & Hage, 1995). Considerations on the factors that need to be pointed out using the zonal QAC are described in publications by prof. D. S. Hage (2002 & Hage, 1995;Yoo et al, 2009, Mallik et al, 2008.…”

“…Such solution has been used in the study of binding of both; hormones (Loun & Hage, 1995) and drugs Yoo et al, 2009;Mallik et al, 2008) to different places in the HSA molecule. Simultaneous injection of imipramine with L-tryptophan on the column with immobilized HSA, showed a competitive interaction between those two drugs, confirming that imipramine specifically connects to the indole-benzodiazepine binding site on the molecule of albumin (Sudlow site II).…”