Characterization of three novel human papillomavirus types isolated from oral rinse samples of healthy individuals

Abstract

Background Despite the strong evidence of HPV infection as the etiological agent in a subset of oral cancer, oral α-HPV detection is rare in healthy individuals, and little is known of the existing of novel HPV types in oral cavity. Objective We determined whether novel HPV types can be isolated from oral rinse samples collected from healthy individuals. Study design We performed rolling circle amplification (RCA) coupled with degenerated PCR assay on 48 oral rinse samples to amplify novel HPV types. Full length HPV DNA was cloned using long range PCR. Quantitative type specific Taqman assays were used to determine the prevalence of novel HPV types in 158 archived oral tissue samples. Results We were able to isolate four novel human papillomavirus types. Full length HPV DNA was cloned for three of the four novel HPV types. All four HPV types belong to the genus Gammapapillomavirus (γ-PV), where HPV 171 is most closely related to HPV 169, showing 88% similarity; HPV 172 is most closely related to HPV 156, showing 70% similarity; HPV 173 is most closely related to HPV 4, showing 73% similarity; oral sample lavage (OSL) 37 is most closely related to HPV 144, showing 69% similarity. Finally, we showed that HPV 173 was rarely present in oral tissues (2/158), HPV 172 was only detected in normal oral tissues (25/76), and HPV 171 was more prevalent in malignant oral tissues (17/82 vs 10/76, p=0.21). Conclusions Novel γ-HPV types are present in oral cavity of healthy individuals.

Characterization of Two Novel Gammapapillomaviruses, HPV179 and HPV184, Isolated from Common Warts of a Renal-Transplant Recipient

“…HPV175 was identified with a metagenomic approach as a single HPV type in a swab sample of a genital wart that had previously tested HPV negative using various broad-range PCR protocols [ 11 ]. HPV156 was initially detected in a sun-exposed healthy skin specimen [ 6 ], while HPV172 was found in an oral rinse sample of a healthy individual [ 55 ]. Based on these data, it seems that at least some of the closest relatives of HPV179 and HPV184 share both cutaneous and mucosal tissue tropism, with some HPV types being causally related to the development of benign lesions.…”

Virus Papiloma Humano en Cáncer Oral y Orofaríngeo: Revisión de la Literatura

“…En el año 1995 la Agencia Internacional para la Investigación sobre el Cáncer (IARC) clasificó los tipos de VPH 16 y 18, como agente carcinógeno humano debido a las oncoproteínas codificadas en su ADN capaces de desregular el ciclo celular (Muñoz et al, 2003;IARC, 2007). Con respecto a la prevalencia a nivel mundial de infección del VPH esta alcanza un 45,8% en orofarínge y 24,2% en cavidad oral (Ndiaye et al, 2014) y es el VPH-16 el más prevalente (Martin et al, 2014). Estructura del VPH.…”

The human urine virome in association with urinary tract infections

“…The HPV genotypes identified in this study have not been associated with high risks for cervical carcinomas, but Type 9 identified in some subjects may be associated with the development of cutaneous cancers (Andersson et al, 2012 ). Other subjects mapped to Type 49, which has been isolated from warts in renal transplant patients (Favre et al, 1989 ), and Type 178, which was isolated from skin adjacent to actinic keratosis (Johansson and Forslund, 2014 ; Martin et al, 2014 ). Our results are among the first to demonstrate the presence of these HPV genotypes in urine, presenting the opportunity to further investigate their role in urological health and diverse urological diseases.…”