Characterization of three mitogen-activated protein kinases (MAPK) genes reveals involvement of ERK and JNK, not p38 in defense against bacterial infection in Yesso scallop Patinopecten yessoensis

Sun, Yan; Zhang, Lingling; Zhang, Meiwei; Li, Ruojiao; Li, Yangping; Hu, Xiaoli; Wang, Shi; Bao, Zhenmin

doi:10.1016/j.fsi.2016.04.139

Cited by 35 publications

(22 citation statements)

References 51 publications

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“…Our results showed that ChERK mRNA was widely distributed in all of the tested developmental stages, with high expression at the trocophore stage (Fig. 4B), which is largely similar to Patinopecten yessoensis ERK [45] and other MAPK members in oyster [40,41]. These expression profiles suggested that ChERK might be similar to homologous genes in other species play a regulatory role in the embryonic development processes of the Hong Kong oyster.…”

Section: Cherk Was Broadly Expressed In Various Tissues and Differentsupporting

confidence: 52%

“…3, ChERK was localized to the mollusk cluster with previously reported ERK sequences from Tegillarca granosa, Crassostrea gigas and Aplysia californica. Multiple sequence alignment and phylogenetic analysis showed that ChERK shared high homology with other reported ERKs, especially with those of mollusk species [44,45], which further demonstrated that ChERK belongs to the ERK family. Previous studies showed that the classic mammalian ERK family includes two highly homologous isoforms (ERK1 and ERK2), whereas only one classic ERK homologue is reported in some lower invertebrate species, including Drosophila melanogaster, Litopenaeus vannamei and Patinopecten yessoensis [34,45e48].…”

Section: Cloning and Characterization Of Cherk Sequencementioning

confidence: 68%

“…4A, ChERK was broadly expressed in all of the tested tissues (gonads, gills, hearts, hemocytes, mantles, adductor muscles, and digestive glands), with a relatively higher expression in digestive glands and hemocytes. Earlier studies suggested that ERK is a widely distributed serine/threonine kinase in various species, such as bony fishes [29,30], arthropods [34,35] and bivalve mollusks [45]. In fishes, yellow croaker ERK was transcribed in almost all of the tissues tested, including intestine, brain, heart, skin, gill, kidney, spleen, head-kidney, stomach, liver, and muscle [30].…”

Section: Cherk Was Broadly Expressed In Various Tissues and Differentmentioning

confidence: 98%

“…6), further indicating that ERK may play an antibacterial role in oysters. Interestingly, a recent study reported that scallop ERK expression was also drastically induced in response to Vibrio anguillarum (G À ) or Micrococcus luteus (G þ ) infections [45], suggesting that the roles of the ERK pathway in defense against Gram-negative and Grampositive bacteria were conserved in scallop and oysters.…”

Section: Expression Of Cherk Was Upregulated After Bacterial Pathogenmentioning

confidence: 99%

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A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro

Xiang

et al. 2017

Fish & Shellfish Immunology

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Section: Cherk Was Broadly Expressed In Various Tissues and Differentsupporting

confidence: 52%

Section: Cloning and Characterization Of Cherk Sequencementioning

confidence: 68%

Section: Cherk Was Broadly Expressed In Various Tissues and Differentmentioning

confidence: 98%

Section: Expression Of Cherk Was Upregulated After Bacterial Pathogenmentioning

confidence: 99%

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A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro

Xiang

et al. 2017

Fish & Shellfish Immunology

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“…TGF-β1, a fibrotic stimulus, is able to activate Smad proteins (Smad2 or Smad3) directly to cause renal fibrosis (31)(32)(33)(34). Mitogen-activated protein kinase (MAPK) pathways, which comprise extracellular signal-regulated kinase (ERK), c-JUN N-terminal kinase (JNK) and p38 signaling, have been reported to play a role in inflammatory regulation (35,36), and also contribute to the amplification of transmitted signals to promote cellular processes, including proliferation and differentiation, either in the cytoplasm or nucleus (37,38). Thus, the current study aimed to investigate two areas: Firstly, whether there is a cross-talk effect between Smad3 and MAPKs in glomerulosclerosis; and secondly, whether the phosphorylation of MAPKs may be mediated by EP4 receptors.…”

Section: Introductionmentioning

confidence: 99%

EP4 knockdown alleviates glomerulosclerosis through Smad and MAPK pathways in mesangial cells

et al. 2018

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Prostaglandin E2 has exhibited pleiotropic effects in the regulation of glomerulosclerosis progression through its four receptors. The current study aimed to evaluate the effect of prostaglandin receptor EP4 on mesangial cell proliferation. In vivo, 5/6 nephrectomy was introduced into EP4 +/and wild-type (WT) mice. Clinical parameters were monitored post-surgery. At 8 weeks post-surgery, glomerular fibrosis-associated indicators were measured by immunohistochemical staining and trichrome staining. In vitro, mesangial cells in different groups (transfected with green fluorescent protein, AD-EF4 or AD-CRE) were exposed to transforming growth factor (TGF)-β1 for 24 h to detect the level of downstream signaling. Corresponding signaling inhibitors were also used to validate the signaling effects. Following surgery, EP4 +/mice presented a higher survival rate and normal urine volume compared with the WT group, and serum creatinine level and 24 h urine protein were lower in the EP4+/-mice. Furthermore, associated profibrotic indicators were identified to have decreased at 8 weeks post-surgery along with less tubule-interstitial fibrosis. In vivo, the inhibition of extracellular signal-regulated kinase and P38 phosphorylation alleviated the accumulation of mesangial matrix, and these signals were enhanced when EP4 was overexpressed. EP4 enhancement aggravated imbalanced mesangial cell proliferation stimulated by TGF-β1 and GS of mice treated with 5/6 nephrectomy through the Smad and mitogen-activated protein kinase pathways.

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Immunity in Molluscs: Recognition and Effector Mechanisms, with a Focus on Bivalvia

Gerdol

Gomez‐Chiarri

Castillo

et al. 2018

Advances in Comparative Immunology

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The study of molluscan immune systems, and in particular those of bivalve molluscs (clams, oysters, scallops, mussels, etc.) has experienced great growth in recent decades, mainly due to the needs of a rapidly growing aquaculture industry to manage the impacts of disease and the application of -omic tools to this diverse group of invertebrate organisms. Several unique aspects of molluscan immune systems highlighted in this chapter include the importance of feeding behavior and mucosal immunity, the discovery of unique levels of diversity in immune genes, and experimental indication of transgenerational immune priming. The development of comparative functional studies using natural and selectively bred disease-resistant strains, together with the potential but yet to be fully developed application of gene-editing technologies, should provide exciting insights into the functional relevance of immune gene family expansion and molecular diversification in bivalves. Other areas of bivalve immunity that deserve further study include elucidation of the process of hematopoiesis, the molecular characterization of hemocyte subpopulations, and the genetic and molecular mechanisms underlying immune priming. While the most important aspects of the immune system of the largest group of molluscs, gastropods (e.g., snails and slugs), are discussed in detail in Chap. 12, we also briefly outline the most distinctive features of the immune system of another fascinating group of marine molluscs, cephalopods, which include invertebrate animals with extraordinary morphological and behavioral complexity.

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Characterization of three mitogen-activated protein kinases (MAPK) genes reveals involvement of ERK and JNK, not p38 in defense against bacterial infection in Yesso scallop Patinopecten yessoensis

Cited by 35 publications

References 51 publications

A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro

A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro

EP4 knockdown alleviates glomerulosclerosis through Smad and MAPK pathways in mesangial cells

Immunity in Molluscs: Recognition and Effector Mechanisms, with a Focus on Bivalvia

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