Characterization of Three Members of Murine α1,2-Fucosyltransferases: Change in the Expression of the Se Gene in the Intestine of Mice after Administration of Microbes

Alterations in the glycolipid composition and cellular properties of ovarian carcinoma‐derived RMG‐1 cells on transfection of the α1,2‐fucosyltransferase gene

“…As reported previously, (15) the glycolipids with terminal galactose in ovarian carcinoma-derived RMG-1 cells were Lc 4 Cer and Le X , which were expected to be converted to H-1 and Le Y glycolipids, respectively, on transfection of the fucosyltransferase gene. Accordingly, selection of transfectants, which were preselected for resistance to G418, was carried out by immunostaining of the cells with anti-Le Y antibody after limiting dilution of the transfectants, and the resultant cell clones of transfectants with a stronger intensity of immunostaining than that of RMG-1 cells were used for analyses of the fucosyltransferase activity and glycolipid composition.…”

“…After incubation at 37°C for 1 h, the reaction was ended with 300 µL of chloroform/ methanol (2:1, v/v), and then the products in the lower phase were separated, using TLC with chloroform/methanol / 0.5% CaCl 2 (55:45:10, v/v). (14,15) The radioactivity incorporated into the glycolipids was determined using a liquid scintillation counter (Tri-Carb 1500; Packard, Foster City, CA, USA).…”

Deficiency of Reproductive Tract α(1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 α(1,2)Fucosyltransferase Locus

“…Interactions between microbes and fucosylated glycans on mammalian host cells extend to the small intestine in rodents, where host-derived ␣(1,2)fucosylated glycans support the growth of and are in turn regulated by Bacteroides thetaiotaomicron (4). In each of these contexts, expression of cell surface fucosylated glycans is directed in part by one or more of the cognate fucosyltransferases responsible for their synthesis (24,25).…”

Molecular Cloning and Characterization of a Novel α1,2-Fucosyltransferase (CE2FT-1) from Caenorhabditis elegans

“…Inoculation of the germ-free mice with Bacteroides thetaiotaomicron significantly stimulated expression of Fuc␣1-2Gal-R structures and transcripts encoding an ␣1,2FT (35), which could be derived from either SEC1 or FUT2 (36). It was recently shown in germ-free mice that administration of intestinal microbes stimulated expression of MFUT-II, the ortholog of human FUCT2 (Se) and rat FTB (37,38). The bacteria-dependent commensal stimulation of host fucosylation is a complex phenomenon involved in generating metabolic fucose to sustain bacterial flora (39).…”