Characterization of three Arabidopsis thaliana immunophilin genes involved in the plant defense response against Pseudomonas syringae

Pogorelko, Gennady; Mokryakova, M. V.; Fursova, Oksana; Абдеева, И. А.; Пирузян, Е. С.; Брускин, С. А.

doi:10.1016/j.gene.2014.01.029

Cited by 52 publications

(56 citation statements)

References 78 publications

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“…In a meta-analysis study, where 17 microarray studies on plant-pathogen interactions were screened for the changes in the expression profiles of PPIases of Arabidopsis thaliana, AtFKBP65 had the most pronounced over-expression rate after pathogen challenge (Pogorelko et al, 2014). AtFKBP65 together with the cyclophilins AtCyp19 and AtCyp57 was shown to be expressed at the site of infection, and promote callose accumulation forming a physical barrier against the invaders.…”

Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

“…AtFKBP65 together with the cyclophilins AtCyp19 and AtCyp57 was shown to be expressed at the site of infection, and promote callose accumulation forming a physical barrier against the invaders. While deletion of the genes resulted in higher susceptibility towards Pseudomonas syringae, transgenic expression of the same genes in tobacco plants provided increased resistance (Pogorelko et al, 2014). How AtFKBP65 and other cyclophilins exert their immunomodulatory actions is not known in detail.…”

Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

“…How AtFKBP65 and other cyclophilins exert their immunomodulatory actions is not known in detail. But in case of AtFKBP65 a direct involvement in gene expression can be speculated, since this modular PPIase is found in the plant cell nucleus, and has tetratricopeptide and calmodulin-binding motifs that mediate protein-protein interactions linked to Ca 2+ -signalling (He et al, 2004;Pogorelko et al, 2014).…”

Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

See 2 more Smart Citations

FKBPs in bacterial infections

Ünal

Steinert

2015

Biochimica et Biophysica Acta (BBA) - General Subjects

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Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

Section: Host Fkbps In Bacterial Infection Processesmentioning

confidence: 99%

See 1 more Smart Citation

FKBPs in bacterial infections

Ünal

Steinert

2015

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…Arabidopsis triple mutants that lack these three genes showed an increased susceptibility to P. syringae compared to wild type, whereas overexpression of these genes in Arabidopsis led to an enhanced resistance to the pathogen. The mechanism of these immunophilins function in this aspect is still unclear, though there is evidence showing that AtCYP19 could be involved in reactive oxygen species production, and both AtCYP57 and AtFKBP65 play a role in the cell (Pogorelko et al 2014 ).…”

Section: At19-3 Atcyp57 and Atfkbp65mentioning

confidence: 99%

Plant Immunophilins: A Protein Family with Diverse Functions Beyond Protein Folding Activity

Shi¹,

Fu²

2015

Elucidation of Abiotic Stress Signaling in Plants

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Immunophilins were discovered as cellular receptors for immunosuppressive drugs: cyclosporine A and FK506. Cyclophilins (CYPs, receptors for cyclosporine A) and FK506-binding proteins (FKBPs, receptors for FK506) do not share sequence homology, but have a common feature of peptidyl-prolyl cis-trans isomerase (PPIase) activity that catalyzes the cis-trans conversion of X-Pro peptide bonds, a rate-limiting step in the process of protein folding. Immunophilins are widely present in organisms from bacteria and fungi, to animals and plants. Genomics studies revealed that plants possess the largest immunophilin family. However, the physiological function of plant immunophilins is poorly understood. In this review, starting with a brief introduction of the immunophilin family and the current knowledge about their physiological roles in diverse organisms, the recent advances in the elucidation of plant immunophilin's physiological roles, largely via functional genomics tools, are summarized here. Notably, a striking feature of plant immunophilins is that a large fraction is localized in the chloroplast. Recent studies reveal that chloroplast immunophilins play a central role in the assembly and maintenance of photosynthetic complexes.

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“…Several reports reveal the involvement of IMMs in response to different types of biotic and abiotic stresses (Ahn et al, 2010;Aviezer-Hagai et al, 2007;Marivet et al, 1992;Meiri et al, 2010;Pogorelko et al, 2014). The expression of IMM genes has also been previously shown to be induced by both biotic and abiotic stresses, including fungal infection (Godoy et al, 2000), viral infection, salicylic acid, ethephon (an ethylene releaser), and HgCl 2 (Marivet et al, 1995), cold shock, salt stress, drought, and heat (Ahn et al, 2010;Marivet et al, 1994;Scholze et al, 1999;Sharma and Singh, 2003).…”

Section: Introductionmentioning

confidence: 98%

Genome Wide Identification of the Immunophilin Gene Family in Leptosphaeria maculans: A Causal Agent of Blackleg Disease in Oilseed Rape (Brassica napus)

Singh

Zouhar²,

Mazáková³

et al. 2014

OMICS: A Journal of Integrative Biology

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Phoma stem canker (blackleg) is a disease of world-wide importance on oilseed rape (Brassica napus) and can cause serious losses for crops globally. The disease is caused by dothideomycetous fungus, Leptosphaeria maculans, which is highly virulent/aggressive. Cyclophilins (CYPs) and FK506-binding proteins (FKBPs) are ubiquitous proteins belonging to the peptidyl-prolyl cis/trans isomerase (PPIase) family. They are collectively referred to as immunophilins (IMMs). In the present study, IMM genes, CYP and FKBP in haploid strain v23.1.3 of L. maculans genome, were identified and classified. Twelve CYPs and five FKBPs were determined in total. Domain architecture analysis revealed the presence of a conserved cyclophilin-like domain (CLD) in the case of CYPs and FKBP_C in the case of FKBPs. Interestingly, IMMs in L. maculans also subgrouped into single domain (SD) and multidomain (MD) proteins. They were primarily found to be localized in cytoplasm, nuclei, and mitochondria. Homologous and orthologous gene pairs were also determined by comparison with the model organism Saccharomyces cerevisiae. Remarkably, IMMs of L. maculans contain shorter introns in comparison to exons. Moreover, CYPs, in contrast with FKBPs, contain few exons. However, two CYPs were determined as being intronless. The expression profile of IMMs in both mycelium and infected primary leaves of B. napus demonstrated their potential role during infection. Secondary structure analysis revealed the presence of atypical eight b strands and two a helices fold architecture. Gene ontology analysis of IMMs predicted their significant role in protein folding and PPIase activity. Taken together, our findings for the first time present new prospects of this highly conserved gene family in phytopathogenic fungus.

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Characterization of three Arabidopsis thaliana immunophilin genes involved in the plant defense response against Pseudomonas syringae

Cited by 52 publications

References 78 publications

FKBPs in bacterial infections

FKBPs in bacterial infections

Plant Immunophilins: A Protein Family with Diverse Functions Beyond Protein Folding Activity

Genome Wide Identification of the Immunophilin Gene Family in Leptosphaeria maculans: A Causal Agent of Blackleg Disease in Oilseed Rape (Brassica napus)

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