Characterization of thermostable recombinant laccase F from Trametes hirsuta and its application in delignification of rice straw

Wang, Chengpeng; Jia, Yitong; Luo, Jingyi; Chen, Bosheng; Pan, Chengyuan

doi:10.1016/j.biortech.2024.130382

Bioresource Technology

2024

DOI: 10.1016/j.biortech.2024.130382

|View full text |Cite

Characterization of thermostable recombinant laccase F from Trametes hirsuta and its application in delignification of rice straw

Chengpeng Wang,

Yitong Jia,

Jingyi Luo

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

UV-ARTP combined mutagenesis selection of lignin-degrading strain and enzymatic characterization of the resultant organisms

Sun,

Zhang,

Liu

et al. 2024

Food Bioscience

View full text Add to dashboard Cite

UV-ARTP combined mutagenesis selection of lignin-degrading strain and enzymatic characterization of the resultant organisms

Sun,

Zhang,

Liu

et al. 2024

Food Bioscience

View full text Add to dashboard Cite

Laccase: Sustainable production strategies, heterologous expression and potential biotechnological applications

Sodhi,

Bhatia,

Batra

2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization

Ortolan,

Contato,

Aranha

et al. 2024

Reactions

View full text Add to dashboard Cite

Trametes hirsuta GMA-01 was cultivated in a culture medium supplemented with orange waste, starch, wheat bran, yeast extract, and salts. The fungus produced several holoenzymes, but the laccase levels were surprisingly high. Given the highlighted applicability of laccases in various biotechnological areas with minimal environmental impact, we provided a strategy to increase its production using response surface methodology. The immobilization of laccase into ionic supports (CM-cellulose, DEAE-agarose, DEAE-cellulose, DEAE-Sephacel, MANAE-agarose, MANAE-cellulose, and PEI-agarose) was found to be efficient and recuperative, showcasing the technical prowess of research. The crude extract laccase (CE) and CM-cellulose-immobilized crude extract (ICE) showed optimum activity in acidic conditions (pH 3.0) and at 70 °C for the CE and 60 °C for the ICE. The ICE significantly increased thermostability at 60 °C for the crude extract, which retained 21.6% residual activity after 240 min. The CE and ICE were successfully applied to sugarcane bagasse hydrolysis, showing 13.83 ± 0.02 µmol mL−1 reducing sugars after 48 h. Furthermore, the CE was tested for dye decolorization, achieving 96.6%, 71.9%, and 70.8% decolorization for bromocresol green, bromophenol blue, and orcein, respectively (0.05% (w/v) concentration). The properties and versatility of T. hirsuta GMA-01 laccase in different biotechnological purposes are interesting and notable, opening several potential applications and providing valuable insights into the future of biotechnological development.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Characterization of thermostable recombinant laccase F from Trametes hirsuta and its application in delignification of rice straw

Cited by 3 publications

References 45 publications

UV-ARTP combined mutagenesis selection of lignin-degrading strain and enzymatic characterization of the resultant organisms

UV-ARTP combined mutagenesis selection of lignin-degrading strain and enzymatic characterization of the resultant organisms

Laccase: Sustainable production strategies, heterologous expression and potential biotechnological applications

Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization

Contact Info

Product

Resources

About