Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains

Rastogi, Gurdeep; Bhalla, Aditya; Adhikari, Akash; Bischoff, Kenneth M.; Hughes, Stephen R.; Christopher, Lew P.; Sani, Rajesh K.

doi:10.1016/j.biortech.2010.06.001

Cited by 248 publications

(195 citation statements)

References 30 publications

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“…Rastogi et al . (2010) reported that Thermobacillus sp. were thermophilic bacteria and could degrade plant cell wall material (the predominant hemicellulose component of a plant cell wall is xylan).…”

Section: Resultsmentioning

confidence: 99%

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

Song

Zhang

Xia

et al. 2018

Microbial Biotechnology

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SummaryIn order to overcome the excessive acidification problem, a microbial consortium for the degradation of organic acids (MCDOA), which acts synergistically in degrading organic acids, was developed and used as an inoculum to improve the efficiency of food waste composting. MCDOA could eliminate the initial lag phase of the pile temperature rise because of excessive acidification and effectively shorten the composting period. Fluorescence regional integration analysis of the excitation‐emission matrix spectra of dissolved organic matter showed that compared with raw material, in compost with MCDOA inoculation, the percent fluorescence response (P i,n) values of Regions I, II and IV decreased by 95.11%, 94.19% and 87.41%, respectively, and P i,n of Region V increased by 172.57%. The decreased and increased levels were markedly higher than in the two control groups (MgO and K2 HPO 4 treatment, and uninoculated compost). These findings revealed that MCDOA accelerated the degradation of proteinaceous compounds and the formation of complicated humic‐like materials. Bacterial profiles implied that MCDOA could improve the indigenous bacterial community structure and diversities of acetic and propionic acid‐degrading and lignin‐degrading bacteria, which might account for the high composting efficiency and degree of humification of the inoculated compost.

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Section: Resultsmentioning

confidence: 99%

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

Song

Zhang

Xia

et al. 2018

Microbial Biotechnology

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“…JAM-KU 023, shown to have maximum cellulolytic activity at the optimal pH range from 7-9.5 (Dave et al, 2015). Since, most of the industrial process involves elevated temperatures, thermostable enzymes stable at elevated temperatures are mostly preferred (Rastogi et al, 2010). The thermostable cellulase enzyme produced by B. licheniformis DR, isolated from hot spring showed optimum cellulase yield at 50°C and the enzyme was found to be stable up to 75°C for 30 min (Irfan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran¹,

Kalaichelv²,

Santhi³

2015

Microbiology J.

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The aim of the present investigation was to exploit the cheap agro-waste, sugarcane bagasse as a substrate for cellulase enzyme production using Bacillus licheniformis MTCC 429. The cellulase producing ability of B. licheniformis MTCC 429 was assessed using CMC agar plates. The factors affecting cellulase production were optimized by varying the parameters of incubation period, temperature and pH. The maximum cellulase enzyme production was achieved when the production medium pH was maintained at pH 7.0, temperature of 35°C with an incubation time of 48 h. Among the different concentration of sugarcane bagasse hydrolysate tested, CMC medium amended with 5% seemed to exhibit maximum cellulase productivity. Further, the partially purified enzyme was tested for its pH and temperature stability. The properties presented by B. licheniformis MTCC 429 suggest that the strain showed enhanced production of lignocellulosedegrading cellulase enzyme under the optimized conditions. The temperature and pH stability of the enzyme suggested its potential for industrial applications involving elevated conditions.

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“…The reaction was stopped by the addition of 3.5-dinitrosalicylic acid (DNS) solution, boiled for 5 min, and then cooled in water. The absorbance was measured at 540 nm in a 5500 spectrophotometer (Christakopoulos et al, 1999;Arikan et al, 2003;Rastogi et al, 2010).…”

Section: Enzyme Assaymentioning

confidence: 99%

A Novel Alkaline, Highly Thermostable and Oxidant Resistant Carboxymethyl Cellulase (Cmcase) Produced by Thermophilic Bacillus sonorensis CY-3

Singh¹,

Singh²

2017

Int.J.Curr.Microbiol.App.Sci

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Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains

Cited by 248 publications

References 30 publications

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

A Novel Alkaline, Highly Thermostable and Oxidant Resistant Carboxymethyl Cellulase (Cmcase) Produced by Thermophilic Bacillus sonorensis CY-3

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