Characterization of Theanine-Forming Enzyme from<i>Methylovorus mays</i>No. 9 in Respect to Utilization of Theanine Production

Yamamoto, Sachiko; Wakayama, Mamoru; Tachiki, Takashi

doi:10.1271/bbb.60590

Cited by 33 publications

(47 citation statements)

References 22 publications

(46 reference statements)

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“…13) This was unsuccessful, however, due to the low reactivity of P. taetrolens Y-30 GS to ethylamine, resulting in difficulty in dissolving large amounts of the enzyme. 14) In a subsequent study, 15) we found a new enzyme with…”

mentioning

confidence: 82%

“…9 was grown at 30 C, as described in a previous paper. 15) E. coli JM109 was used as the host for most DNA manipulations with pUC18 and pET21a. E. coli AD494 (DE3) and pET21a were used in the production of recombinant GMAS.…”

Section: Methodsmentioning

confidence: 99%

“…Several peptide bands detected on the membrane with 0.1% ponceau S were cut out, and their N-terminal amino acid sequences were determined by methods described previously. 15) The sequence was compared with the FASTA program of the DDBJ homepage (http://www.ddbj.nig.ac.jp/ search/fasta-j.html).…”

Section: Methodsmentioning

confidence: 99%

“…10,11) In order to overcome these shortcomings, we have investigated theanine production from glutamic acid and ethylamine using the theanine synthetase-like enzyme, in which sugar fermentation of dried baker's yeast cells was coupled as an ATP-regenerating system (coupled fermentation with energy transfer). [12][13][14][15] In earlier reports, [12][13][14] we indicated the principle of coupled fermentation with energy transfer using the glutamine synthetase (GS; L-glutamate:ammonia ligase, ADP-forming, E.C. 6.3.1.2, Fig.…”

mentioning

confidence: 99%

“…flagellatus KT. 15) In 1995, Merrick et al classified GS into four types, depending on several properties, GS I, GS II, GS III, and Gln T. 17) Overall sequence identity in these GS family is low (less than 35% identity), but the critical amino acids and the five regions, which are involved in the catalytic function and the structural features, are highly conserved in all types of GSs. 18) GS I, encoded by the glnA gene, is widely distributed among bacteria, and has been characterized in detail.…”

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confidence: 99%

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Cloning and Expression ofMethylovorus maysNo. 9 Gene Encoding γ-Glutamylmethylamide Synthetase: An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker’s Yeast

Yamamoto

Wakayama

Tachiki

2008

Bioscience, Biotechnology, and Biochemistry

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mentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Cloning and Expression ofMethylovorus maysNo. 9 Gene Encoding γ-Glutamylmethylamide Synthetase: An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker’s Yeast

Yamamoto

Wakayama

Tachiki

2008

Bioscience, Biotechnology, and Biochemistry

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Methylovorus

Доронина¹,

Капаруллина²,

Agafonova³

2021

Bergey's Manual of Systematics of Archaea and Bacteria

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Me.thy.lo.vo'rus. N.L. neut. n. methylum the methyl group; N.L. masc. adj. vorus consuming; N.L. masc.n. Methylovorus the methyl consumer. Proteobacteria / Betaproteobacteria / Methylophilales / Methylophilaceae / Methylovorus The genus Methylovorus accommodates obligate and restricted facultative methylotrophs. Cells of these methylotrophic bacteria are Gram‐stain negative, aerobic, and motile rods with single polar flagellum, which multiply by binary fission and occur singly without aggregation. Cells do not possess endospores, intracellular membranes, or poly‐β‐hydroxybutyrate granules and prosthecae. Cells of some strains produce slime. Members of this genus are able to utilize methanol via the ribulose monophosphate (RuMP) pathway of C 1 assimilation. These bacteria possess an incomplete tricarboxylic acid cycle because they lack α‐ketoglutarate dehydrogenase. Enzymes of the glyoxylate cycle (malate synthase and isocitrate lyase) are absent. Some strains can grow on methylamine and poorly on glucose or fructose. No growth occurs on rich media with peptone and under CH 4 + O 2 or H 2 + CO 2 + O 2 in the gas phase. These methylotrophic bacteria are mesophilic, neutrophilic, and nonhalophilic. Vitamins or other growth factors are not required for growth. Nitrogen sources are ammonium salts, nitrates, and some amino acids. Indole is produced from l ‐tryptophan on mineral medium with methanol and KNO 3 . The major fatty acids are C 16:0 and C 16:1 ω7 c . Known habitats are wastewater, soil, or phyllosphere and rhizosphere of various plants. DNA G + C content (mol%) : 54.0–55.4 ( T m and genome sequence). Type species : Methylovorus glucosotrophus Govorukhina and Trotsenko 1991 VP .

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γ‐Glutamylation of Isopropylamine by Fermentation

Benninghaus,

Zagami,

Tassini

et al. 2023

ChemBioChem

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Glutamylation yields N‐functionalized amino acids in several natural pathways. γ‐Glutamylated amino acids may exhibit improved properties for their industrial application, e. g., as taste enhancers or in peptide drugs. γ‐Glutamyl−isopropylamide (GIPA) can be synthesized from isopropylamine (IPA) and l‐glutamate. In Pseudomonas sp. strain KIE171, GIPA is an intermediate in the biosynthesis of l‐alaninol (2‐amino‐1‐propanol), a precursor of the fluorochinolone antibiotic levofloxacin and of the chloroacetanilide herbicide metolachlor. In this study, fermentative production of GIPA with metabolically engineered Pseudomonas putida KT2440 using γ‐glutamylmethylamide synthetase (GMAS) from Methylorubrum extorquens was established. Upon addition of IPA during growth with glycerol as carbon source in shake flasks, the recombinant strain produced up to 21.8 mM GIPA. In fed‐batch bioreactor cultivations, GIPA accumulated to a titer of 11 g L−1 with a product yield of 0.11 g g−1 glycerol and a volumetric productivity of 0.24 g L−1 h−1. To the best of our knowledge, this is the first fermentative production of GIPA.

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Characterization of Theanine-Forming Enzyme fromMethylovorus maysNo. 9 in Respect to Utilization of Theanine Production

Cited by 33 publications

References 22 publications

Cloning and Expression ofMethylovorus maysNo. 9 Gene Encoding γ-Glutamylmethylamide Synthetase: An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker’s Yeast

Cloning and Expression ofMethylovorus maysNo. 9 Gene Encoding γ-Glutamylmethylamide Synthetase: An Enzyme Usable in Theanine Formation by Coupling with the Alcoholic Fermentation System of Baker’s Yeast

Methylovorus

γ‐Glutamylation of Isopropylamine by Fermentation

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