Characterization of the virulence, agr typing and antimicrobial resistance profile of Staphylococcus aureus strains isolated from food handlers in Brazil

Pereira, Giovana do Nascimento; Rosa, Rafael da Silva; Dias, André Aparecido; Santos, Diego Júnior; Seribelli, Amanda Aparecida; Pinheiro-Hubinger, Luiza; Winkelströter, Lizziane Kretli; Carvalho, Thaís Batista de; Pereira, Valéria Cataneli

doi:10.1016/j.bjid.2022.102698

Cited by 3 publications

(4 citation statements)

References 47 publications

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“…Foodborne illnesses are a major global health concern, impacting around 600 million individuals annually [ 4 ]. They occur when contaminated food is consumed and they can lead to serious consequences.…”

Section: Introductionmentioning

confidence: 99%

“…Staphylococcus aureus is a pathogenic microorganism known for causing such illnesses, particularly through the production of enterotoxins. These toxins have been linked to outbreaks of foodborne diseases associated with cheese consuming of [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Another significant concern in food safety is antimicrobial resistance (AMR), which results from misuse and abuse of antimicrobial agents in both humans and animals [ 9 ]. In 2019, antibiotic-resistant bacterial infections caused over 1.2 million deaths [ 10 ], with methicillin-resistant S. aureus (MRSA) alone accounting for 100,000 deaths and being a major cause of severe foodborne outbreaks [ 4 ]. Therefore, the objectives of this study were to assess: (i) the microbiological quality of raw sheep’s milk and cheese produced on farms in southern Brazil; (ii) the presence of Staphylococcus spp.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is crucial to assess the presence of microorganisms that indicate the cleanliness and safety of milk to prevent foodborne illnesses. Foodborne illnesses are a major global health concern, impacting around 600 million individuals annually [4]. They occur when contaminated food is consumed and they can lead to serious consequences.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Enterotoxins and Antimicrobial Resistance in Microorganisms Isolated from Raw Sheep Milk and Cheese: Ensuring the Microbiological Safety of These Products in Southern Brazil

Endres,

Moreira,

de Freitas

et al. 2023

Microorganisms

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This study emphasizes the importance of monitoring the microbiological quality of animal products, such as raw sheep’s milk and cheese, to ensure food safety. In Brazil, there is currently no legislation governing the quality of sheep’s milk and its derivatives. Therefore, this study aimed to evaluate: (i) the hygienic-sanitary quality of raw sheep’s milk and cheese produced in southern Brazil; (ii) the presence of enterotoxins and Staphylococcus spp. in these products; and (iii) the susceptibility of the isolated Staphylococcus spp. to antimicrobial drugs and the presence of resistance genes. A total of 35 samples of sheep’s milk and cheese were examined. The microbiological quality and presence of enterotoxins were accessed using Petrifilm and VIDAS SET2 methods, respectively. Antimicrobial susceptibility tests were conducted using VITEK 2 equipment and the disc diffusion method. The presence of resistance genes tet(L), sul1, sul2, ermB, tetM, AAC(6)’, tetW, and strA were evaluated through PCR. In total, 39 Staphylococcus spp. were obtained. The resistance genes tetM, ermB, strA, tetL, sul1, AAC(6)’, and sul2 were detected in 82%, 59%, 36%, 28%, 23%, 3%, and 3% of isolates, respectively. The findings revealed that both raw sheep’s milk and cheese contained Staphylococcus spp. that exhibited resistance to antimicrobial drugs and harbored resistance genes. These results underscore the immediate need for specific legislation in Brazil to regulate the production and sale of these products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Enterotoxins and Antimicrobial Resistance in Microorganisms Isolated from Raw Sheep Milk and Cheese: Ensuring the Microbiological Safety of These Products in Southern Brazil

Endres,

Moreira,

de Freitas

et al. 2023

Microorganisms

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Microbiological Safety of Raw Sheep’s Milk and Cheese Produced in Southern Brazil: An Evaluation of Enterotoxins and Antimicrobial Resistance in Isolated Staphylococcus Species

Endres¹,

Moreira²,

Freitas³

et al. 2023

Preprint

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This work highlights that monitoring the microbiological quality of animal products, such as raw sheep’s milk and cheese, is necessary for food safety. Sheep’s milk and its derivatives are not legislated in Brazil. Thus, present study aimed to evaluate: (i) the hygienic-sanitary quality of raw sheep’s milk and cheese produced in southern Brazil; (ii) the presence of enterotoxins and Staphylococcus spp. in these products; and (iii) the antimicrobial susceptibility profile and resistance genes of the isolated Staphylococcus spp. Thirty-five raw sheep’s milk and cheese samples were evaluated. Microbiological quality and the presence of enterotoxins were determined by Petrifilm and VIDAS SET2 methods, respectively. Antimicrobial susceptibility tests were conducted using VITEK 2 equipment and the disc diffusion method. The presence of resistance genes tet(L), sul1, sul2, ermB, tetM, AAC(6)', tetW, and strA were evaluated by PCR. In total, 39 Staphylococcus spp. were isolated. The tetM, ermB, strA, tetL, sul1, AAC(6)’, and sul2 resistance genes were detected in 82%, 59%, 36%, 28%, 23%, 3%, and 3% of isolates, respectively. The results showed that both products contained Staphylococcus spp. and these strains were resistant to antimicrobials as well carriers of resistance genes. These results highlight Brazil's need for specific legislation regarding the pro-duction and marketing of these products.

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Characterization of Clonal Groups of Antibiotic-Resistant Biofilm-Forming Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infections in Isfahan, Iran

Mostafavi,

Rahimi,

Khashei

2024

Arch Clin Infect Dis

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Background: Over the past decades, the role of biofilm-forming Staphylococcus aureus strains in urinary tract infections (UTIs) has garnered significant attention. Objectives: This study aimed to determine the epidemiological characteristics and diversity of S. aureus strains isolated from patients with UTIs in Isfahan, Iran, in 2017, with regard to their antimicrobial resistance, biofilm formation, and phylogenetic profiles. Additionally, the study investigated potential relationships among these factors statistically to develop efficient control and treatment approaches. Methods: All patients with symptomatic UTIs who had positive urine cultures for S. aureus during the study period at the laboratory of a referral hospital in Isfahan were included. All isolates were identified using specific primers for the nucA gene. Their biofilm formation capacity was evaluated using a combination of the microtiter plate and Congo-red agar methods. Antibiotic susceptibility testing was performed using the disk diffusion method. The presence of genes involved in biofilm formation and resistance to cefoxitin, aminoglycosides, and fluoroquinolones was detected using polymerase chain reaction (PCR). Staphylococcal cassette chromosome mec (SCCmec) typing, agr typing, and phene plate (PhP) typing were employed to investigate the diversity of collected strains. Results: Results showed that 19%, 57%, and 24% of confirmed S. aureus strains were strong, intermediate, and non-biofilm formers, respectively. The highest rate of resistance was against nalidixic acid (77%), followed by streptomycin (73%). The icaD and icaA genes had the highest frequency among biofilm-producing strains. gyrA (44%) and grlA (35%) were the most frequent genes among fluoroquinolone-resistant strains, while aph(3′)-IIIa was the most prevalent aminoglycoside-modifying enzyme gene. The majority of bacterial strains harbored SCCmec type III and agr type I. PhP typing of strains revealed the presence of 8 common types (CTs) and 14 single types (STs), with CT2 being the dominant type. Conclusions: The present investigation revealed various biofilm production capacities, antimicrobial resistance profiles, and clonal lineages in S. aureus isolated from patients with UTIs. These findings provide further insights into the epidemiology and pathogenicity of S. aureus strains in Iran, thereby improving the quality of surveillance and therapeutic protocols.

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Characterization of the virulence, agr typing and antimicrobial resistance profile of Staphylococcus aureus strains isolated from food handlers in Brazil

Cited by 3 publications

References 47 publications

Evaluation of Enterotoxins and Antimicrobial Resistance in Microorganisms Isolated from Raw Sheep Milk and Cheese: Ensuring the Microbiological Safety of These Products in Southern Brazil

Evaluation of Enterotoxins and Antimicrobial Resistance in Microorganisms Isolated from Raw Sheep Milk and Cheese: Ensuring the Microbiological Safety of These Products in Southern Brazil

Microbiological Safety of Raw Sheep’s Milk and Cheese Produced in Southern Brazil: An Evaluation of Enterotoxins and Antimicrobial Resistance in Isolated Staphylococcus Species

Characterization of Clonal Groups of Antibiotic-Resistant Biofilm-Forming Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infections in Isfahan, Iran

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