“…All salts, drugs and reagents are of analytical purity, and were obtained from Sigma-Aldrich Corporation (St. Louis, Missouri, USA), Merck (Darmstadt, Germany) and Reagens (Rio de Janeiro, RJ, Brazil), and stored according to the manufacturer's instructions. The Krebs-Henseleit solution used in the experiments followed the composition (in mmol/L) provided in Dantas et al [ 16 ]: KCl (4.8), NaCl (125), MgSO 4 (1.2), CaCl 2 (1), glucose (11), KH 2 PO 4 (1.2), NaHCO 3 (25) and EDTA (0.3). This solution was also modified for transport/storage with the addition of HEPES (25), and for certain experimental evaluations, a Krebs-Henseleit solution with 60 mmol/L was prepared by equimolar substitution of KCl and NaCl.…”