Characterization of the vasodilator effect of eugenol in isolated human umbilical cord arteries

Vasodilation promoted by (E,E)-farnesol involving ion channels in human umbilical arteries

“…This is the first publication describing the vasodilatory effect of ( E , E )-FAR on HUA from healthy women. Our main findings show that ( E , E )-FAR promotes reversible relaxation in basal tone of HUA, and is able to reduce all contractile activity induced by depolarization (KCl 60 mmol/L) or pharmacological agonism (5-HT 10 μmol/L), with higher potency in the electromechanical pathway, even compared with the relaxing pharmacological potencies of other natural products in HUA [ 16 , 17 ].…”

“…All salts, drugs and reagents are of analytical purity, and were obtained from Sigma-Aldrich Corporation (St. Louis, Missouri, USA), Merck (Darmstadt, Germany) and Reagens (Rio de Janeiro, RJ, Brazil), and stored according to the manufacturer's instructions. The Krebs-Henseleit solution used in the experiments followed the composition (in mmol/L) provided in Dantas et al [ 16 ]: KCl (4.8), NaCl (125), MgSO 4 (1.2), CaCl 2 (1), glucose (11), KH 2 PO 4 (1.2), NaHCO 3 (25) and EDTA (0.3). This solution was also modified for transport/storage with the addition of HEPES (25), and for certain experimental evaluations, a Krebs-Henseleit solution with 60 mmol/L was prepared by equimolar substitution of KCl and NaCl.…”

“…In the laboratory, the segments were dissected to remove Wharton's jelly and obtain the umbilical arteries, which were then sectioned into rings (3–5 mm). The rings had their vascular endothelium mechanically removed as previously described [ 16 , 17 ] and were immediately suspended in glass chambers (10 mL) of organ bath equipment filled with Krebs-Henseleit under the ideal conditions cited previously, to record the isometric tension of the rings by force transducers (MLT0420, ADInstruments Bridge Amps, ADInstruments, Sydney, Australia).…”

“…The arterial rings were artificially tensioned at 3 gf for 90 min, during which time the Krebs-Henseleit solution was renewed every 15 min to avoid interference from metabolites [ 16 ]. After stabilization, the rings were qualified as viable for functional protocols when they assumed a contractile response ≥1 gf after stimulation with 60 mmol/L KCl (K60), otherwise they were discarded.…”

“…Moreover, researches evaluating the action of natural products on the smooth muscle of these vessels are scarce worldwide [ 16 ]. Thus, this study aims to characterize, for the first time, the vasodilatory effect of ( E , E )-FAR on healthy women's HUA.…”