Characterization of the Trr/Trx system in the fungal pathogen Candida glabrata

Gutiérrez-Escobedo, Guadalupe; Vázquez-Franco, Norma; López-Marmolejo, Ana; Luna-Arvizu, Gabriel; Cañas-Villamar, Israel; Castaño, Irene; Peñas, Alejandro De Las

doi:10.1016/j.fgb.2023.103799

Cited by 2 publications

(1 citation statement)

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“…YAP1 also promotes the transcription of several genes involved in the thioredoxin (Trx) and GSH/glutaredoxin (Grx) systems. [35] Accordingly, the sensitivity of mutant strains with damaged Trx or Grx systems to CQ was observed. It was observed that CQ did not display protective effects in the mutants with the disruption of Trx or Grx system (Figure 5F), both of which help maintain cell GSH redox status.…”

Section: Cq Restores Intracellular Reduced Glutathione Content In Yea...mentioning

confidence: 99%

Clioquinol rescues yeast cells from Aβ42 toxicity via the inhibition of oxidative damage

Zheng,

Zhu,

et al. 2024

Biotechnology Journal

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Alzheimer's disease (AD), the most common form of dementia, has gotten considerable attention. Previous studies have demonstrated that clioquinol (CQ) as a metal chelator is a potential drug for the treatment of AD. However, the mode of action of CQ in AD is still unclear. In our study, the antioxidant effects of CQ on yeast cells expressing Aβ42 were investigated. We found that CQ could reduce Aβ42 toxicity by alleviating reactive oxygen species (ROS) generation and lipid peroxidation level in yeast cells. These alterations were mainly attributable to the increased reduced glutathione (GSH) content and independent of activities of superoxide dismutase (SOD) and/or catalase (CAT). CQ could affect antioxidant enzyme activity by altering the transcription level of related genes. Interestingly, it was noted for the first time that CQ could combine with antioxidant enzymes to reduce their enzymatic activities by molecular docking and circular dichroism spectroscopy. In addition, CQ restored Aβ42‐mediated disruption of GSH homeostasis via regulating YAP1 expression to protect cells against oxidative stress. Our findings not only improve the current understanding of the mechanism of CQ as a potential drug for AD treatment but also provide ideas for subsequent drug research and development.

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Section: Cq Restores Intracellular Reduced Glutathione Content In Yea...mentioning

confidence: 99%