Characterization of the transport of lysine-containing dipeptides by PepT1 orthologs expressed in Xenopus laevis oocytes

Margheritis, Eleonora; Terova, Genciana; Oyadeyi, Ayodele Stephen; Renna, Maria Daniela; Cinquetti, Raffaella; Peres, Antonio; Bossi, Elena

doi:10.1016/j.cbpa.2012.12.016

Cited by 19 publications

(17 citation statements)

References 38 publications

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“…Furthermore, present study demonstrated that compared with the FM diet, MBM diet increased the gene expression of intestinal PepT1, which was agreed with other findings in turbot (Wei, Liang, Mu, Zheng & Xu 2015), but was dissimilar to previous studies on Sea Bass (Dicentrarchus labrax) (Terova et al 2013) and Yellow Perch (Perca flavescens) (Ostaszewska, Dabrowski, Kamaszewski, Kwasek, Grodzik & Bierla 2013). So far, research on PepT1 has revealed that species-specific differences existed among different animals in its expression levels (Margheritis, Terova, Oyadeyi, Renna, Cinquetti, Peres & Bossi 2013). Intestinal gene expression of AA transporters had the same pattern with the PepT1.…”

Section: Discussionsupporting

confidence: 64%

“…) and Yellow Perch ( Perca flavescens ) (Ostaszewska, Dabrowski, Kamaszewski, Kwasek, Grodzik & Bierla ). So far, research on PepT1 has revealed that species‐specific differences existed among different animals in its expression levels (Margheritis, Terova, Oyadeyi, Renna, Cinquetti, Peres & Bossi ). Intestinal gene expression of AA transporters had the same pattern with the PepT1.…”

Section: Discussionmentioning

confidence: 99%

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The differences in postprandial free amino acid concentrations and the gene expression of PepT1 and amino acid transporters after fishmeal partial replacement by meat and bone meal in juvenile turbot (Scophthalmus maximus L.)

Song

Zhou

et al. 2016

Aquac Res

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Meat and bone meal (MBM) is a high‐quality alternative protein source used to replace fishmeal (FM). However, the molecular mechanisms of over‐substituted FM by MBM resulted in growth reduction are still not clear. The objective of the study was to evaluate the effect of FM replacement by MBM on the concentration of postprandial free amino acid (FAA) and mRNA abundance of peptide and amino acid transporters in juvenile turbot (Scophthalmus maximus L.). Fish were fed with FM diet (60% FM), MBM diet (33% FM + 34.2% MBM) and MBM + AA diet (MBM diet with essential amino acid (EAA) added to match the AA profile of FM) for 30 days. Results showed that compared with the FM diet, MBM diet led to a reduction in FAA concentration peak values in plasma and muscle. MBM + AA diet significantly elevated the peak values of FAA concentrations to FM diet level in plasma, but not in muscle. Furthermore, compared with FM diet, MBM diet significantly increased gene expression of PepT1 and major amino acid transporters in intestine, whereas MBM diet greatly downregulated gene expression of T‐type amino acid transporter‐1, system ASC amino acid transporter‐2 and cationic amino acid transporter‐2 in muscle. Supplemented EAA did not ameliorate these different effects in intestine and muscle. Overall, this study provided a comprehensive explanation for the relationship between diet, FAA concentrations and AA transportations, which provides a molecular basis for further using MBM to replace FM in aquafeeds.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

The differences in postprandial free amino acid concentrations and the gene expression of PepT1 and amino acid transporters after fishmeal partial replacement by meat and bone meal in juvenile turbot (Scophthalmus maximus L.)

Song

Zhou

et al. 2016

Aquac Res

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“…The intestinal PepT1 are responsible for the uptake of a large portion of amino acids as di-and tripeptide and free amino acids are taken up by amino acid transporters (Fei et al 1994). The current result revealed that it was possible that species-specific differences were observed for the PepT1 in response to various substrates (Margheritis et al 2013). Furthermore, there was no relationship between the growth of turbot and PepT1 expression level.…”

Section: Discussionmentioning

confidence: 60%

“…The current result revealed that it was possible that species‐specific differences were observed for the PepT1 in response to various substrates (Margheritis et al . ). Furthermore, there was no relationship between the growth of turbot and PepT1 expression level.…”

Section: Discussionmentioning

confidence: 97%

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Wei

Liang

et al. 2015

Aquacult Nutr

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The intention of the study was to investigate the effect of ultrafiltered fish protein hydrolysate (UF) level on growth, feed utilization, apparent digestibility coefficients and proximal intestine peptide transporter 1 (PepT1) mRNA level for juvenile turbot (Scophthalmus maximus L.). Experimental diets (UF‐0, UF‐5, UF‐10, UF‐15 and UF‐20) were prepared containing about 68% plant protein, and fish meal protein was, respectively, replaced by 0%, 5%, 10%, 15% and 20% UF of dietary protein. Diet PP contained about 78% plant protein, and diet CAA contained about 10% crystalline amino acid mixture. All diets were fed to seven triplicate groups of turbot (initial weight 16.05 ± 0.03 g) for 68 days. Fish fed diet UF‐10 had an increasing tendency in growth compared with diets contained UF, while dietary UF level was not significantly correlated with specific growth rate and feed intake. Feed efficiency, protein efficiency ratio and protein productive value significantly correlated with dietary UF level, and fish fed diets contained low‐level UF had higher digestibility than that diets UF‐0, PP and CAA. There was a decreasing tendency in PepT1 expression level with dietary UF level. The results indicated that low‐level UF showed a positive effect on growth and feed utilization in juvenile turbot.

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“…The possible reason was that these fish species may have the different ability to use the same dipeptide. This may be supported by the fact that species‐specific differences were observed for transport efficiency of dipeptides by PepT1 (Margheritis et al, ). In addition, the present research had also investigated the effect of synthetic tripeptide in diets on the growth and feed utilization of turbot.…”

Section: Discussionmentioning

confidence: 99%

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

Wei

Liang

2019

Aquacult Nutr

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The study aimed to investigate the effects of crystalline Lys and Leu, Lys‐Leu dipeptide and Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide, on growth, postprandial Lys and Leu concentrations, gene expression of peptide and amino acid (AA) transporters and related gene expression of protein synthesis pathway in turbot. Three diets (CAA, Di and Tri) contained Lys and Leu as free (Lys and Leu), dipeptide (Lys‐Leu) and tripeptide (Lys‐Leu‐Lys/Leu‐Lys‐Leu) forms, respectively. Improved growth was observed in the Di group compared with the CAA group. For peptide and AAs transporters, PepT1, B0AT1 and y+LAT2 mRNA levels were affected in proximal intestine by dietary treatments. Free Lys and Leu concentrations in the CAA group were significantly higher than that of the Di and Tri groups at 6 hr postfeeding in serum and at 2, 6 and 24 hr postfeeding in muscle. For protein synthesis pathway in muscle, Akt2, TOR and S6k1 gene expression were the highest in the Di group and the lowest in the CAA group, whereas MuRF1 relative expression was the highest in the Tri group. In conclusion, dietary Lys‐Leu dipeptide was utilized more efficiently than free Lys and Leu or Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide for turbot by regulating AAs transport, postprandial AAs concentration and the synthesis of muscle proteins.

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Characterization of the transport of lysine-containing dipeptides by PepT1 orthologs expressed in Xenopus laevis oocytes

Cited by 19 publications

References 38 publications

The differences in postprandial free amino acid concentrations and the gene expression of PepT1 and amino acid transporters after fishmeal partial replacement by meat and bone meal in juvenile turbot (Scophthalmus maximus L.)

The differences in postprandial free amino acid concentrations and the gene expression of PepT1 and amino acid transporters after fishmeal partial replacement by meat and bone meal in juvenile turbot (Scophthalmus maximus L.)

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

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