Characterization of the Transcriptome and Gene Expression of Brain Tissue in Sevenband Grouper (Hyporthodus septemfasciatus) in Response to NNV Infection

Kim, Jong‐Oh; Kim, Jae-Ok; Kim, Wi‐Sik; Oh, Myung‐Joo

doi:10.3390/genes8010031

Cited by 23 publications

(15 citation statements)

References 57 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, a significant up-regulation of antiviral proteins and NK-Lysin, a known antibacterial protein, was observed in the brain of NNV infected sevenband grouper (E. septemfaciatus). Furthermore, several chemokines, cathepsins and lepsins were also up-regulated [239]. Brain tissue was also analyzed in persistently infected Malabar grouper (E. malabaricus), showing that highly immune cell active signaling and surface receptor expression were triggered during persistent infection, as well as the interferon-induced response [236].…”

Section: Host Responsementioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

View full text Add to dashboard Cite

The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

show abstract

Section: Host Responsementioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

View full text Add to dashboard Cite

show abstract

“…Such investigations have been performed in grouper kidney cells (GK cell line) [5], Asian sea bass epithelial cells (SB cell line) [6], European sea bass leukocytes [7], striped snakehead fish cells (SSN-1 cell line) [8] and European sea bass brain cells (DLB-1 cell line) [9]. The in vivo effect of NNV has also been analysed by RNA-Seq in the brain of sevenband grouper [10], pooled brain/eye and head kidney samples from Senegalese sole [11], the brain of Malabar grouper [12], and the liver, spleen and kidney of Epinephelus moara [13]. However, the in vivo response of European sea bass remains almost completely unexplored, and only a small number of publications have reported the modulation or involvement of immune factors in different tissues of D. labrax infected with NNV [14][15][16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq analysis of European sea bass (Dicentrarchus labrax L.) infected with nodavirus reveals powerful modulation of the stress response

Lama

Pereiro

Valenzuela-Muñoz

et al. 2020

Vet Res

View full text Add to dashboard Cite

Nodavirus, or nervous necrosis virus (NNV), is the causative agent of viral encephalopathy and retinopathy (VER), a severe disease affecting numerous fish species worldwide. European sea bass, a cultured species of great economic importance, is highly susceptible to the disease. To better understand the response of this organism to NNV, we conducted RNA-Seq analysis of the brain and head kidney from experimentally infected and uninfected sea bass juveniles at 24 and 72 hours post-infection (hpi). Contrary to what was expected, we observed modest modulation of immune-related genes in the brain, the target organ of this virus, and some of these genes were even downregulated. However, genes involved in the stress response showed extremely high modulation. Accordingly, the genes encoding the enzymes implicated in the synthesis of cortisol were almost the only overexpressed genes in the head kidney at 24 hpi. This stress response was attenuated after 72 h in both tissues, and a progressive immune response against the virus was mounted. Moreover, experiments were conducted to determine how stress activation could impact NNV replication. Our results show the complex interplay between viral activity, the stress reaction and the immune response.

show abstract

“…The number of down-regulated genes was higher than up-regulated genes, suggesting that virus infection affects the expression of genes and lead to dysfunction. In another study, 628 DEGs were identified in the adult sevenband grouper after NNV infection (Kim et al 2017). The DEGs in this study will help identify causative genes for NNV resistance.…”

Section: Degs Of Brain Rna-seq Located In the 17 Mbp Region Involved In Immunity And Neuron Developmentmentioning

confidence: 79%

“…In this study, the number of reads was greater than the RNA-seq reads from other studies. For examples, about 45.1 million reads per library is obtained in sevenband grouper (Hyporthodus septemfasciatus) brain RNA-seq for NNV infection (Kim et al 2017). Therefore, the number of reads was sufficient for subsequent analysis to identify DEGs in brain RNA-seq.…”

Section: Data Acquisition Of Asian Seabass Brain Rna-seq After Nnv Challengementioning

confidence: 99%

Effects of rrm1 on NNV Resistance Revealed by RNA-seq and Gene Editing

2021

View full text Add to dashboard Cite

Viral nervous necrosis (VNN) disease caused by the nervous necrosis virus (NNV) is a major disease, leading to a huge economic loss in aquaculture. Previous GWAS and QTL mapping have identified a major QTL for NNV resistance in linkage group 20 in Asian seabass. However, no causative gene for NNV resistance has been identified. In this study, RNA-seq from brains of Asian seabass fingerlings challenged with NNV at four time points (5, 10, 15 and 20 days post-challenge) identified 1228, 245, 189 and 134 DEGs, respectively. Eight DEGs, including rrm1, were located in the major QTL for NNV resistance. An association study in 445 survived and 608 dead fingerlings after NNV challenge revealed that the SNP in rrm1 were significantly associated with NNV resistance. Therefore, rrm1 was selected for functional analysis, as a candidate gene for NNV resistance. The expression of rrm1 was significantly increased in the gill, liver, spleen and muscle, and was suppressed in the brain, gut and skin after NNV challenge. The rrm1 protein was localized in the nuclear membrane. Overexpression of rrm1 significantly decreased viral RNA and titer in NNV-infected Asian seabass cells, whereas knock-down of rrm1 significantly increased viral RNA and titer in NNV-infected Asian seabass cells. The rrm1 knockout heterozygous zebrafish was more susceptible to NNV infection. Our study suggests that rrm1 is one of the causative genes for NNV resistance and the SNP in the gene may be applied for accelerating genetic improvement for NNV resistance.

show abstract

Characterization of the Transcriptome and Gene Expression of Brain Tissue in Sevenband Grouper (Hyporthodus septemfasciatus) in Response to NNV Infection

Cited by 23 publications

References 57 publications

Betanodavirus and VER Disease: A 30-year Research Review

Betanodavirus and VER Disease: A 30-year Research Review

RNA-Seq analysis of European sea bass (Dicentrarchus labrax L.) infected with nodavirus reveals powerful modulation of the stress response

Effects of rrm1 on NNV Resistance Revealed by RNA-seq and Gene Editing

Contact Info

Product

Resources

About