Characterization of the Technofunctional Properties and Three-Dimensional Structure Prediction of 11S Globulins from Amaranth (Amaranthus hypochondriacus L.) Seeds

Aguilar-Padilla, Jorge; Centeno-Leija, Sara; Bojórquez‐Velázquez, Esaú; Elizalde‐Contreras, José M.; Ruiz‐May, Eliel; Serrano‐Posada, Hugo; Osuna-Castro, Juan Alberto

doi:10.3390/foods12030461

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…The changes in H 0 associated with the open-to-closed conformational changes of productive MdxE-ligand complexes were determined based on the method described previously [ 47 , 57 ], using a Nanodrop ND-3300 fluorospectrometer (Thermo Fisher, Waltham, MA, USA) controlled by ND-3300 v2.8.0 software with ANS as a fluorescence probe at an excitation wavelength of 365 nm and 400–600 nm as the range for emission scan. The fluorescent response was measured in RFU using buffer C for all solutions.…”

Section: Methodsmentioning

confidence: 99%

Binding Specificity of a Novel Cyclo/Maltodextrin-Binding Protein and Its Role in the Cyclodextrin ABC Importer System from Thermoanaerobacterales

Aranda-Caraballo,

Saenz,

López-Zavala

et al. 2023

Molecules

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Extracellular synthesis of functional cyclodextrins (CDs) as intermediates of starch assimilation is a convenient microbial adaptation to sequester substrates, increase the half-life of the carbon source, carry bioactive compounds, and alleviate chemical toxicity through the formation of CD-guest complexes. Bacteria encoding the four steps of the carbohydrate metabolism pathway via cyclodextrins (CM-CD) actively internalize CDs across the microbial membrane via a putative type I ATP-dependent ABC sugar importer system, MdxEFG-(X/MsmX). While the first step of the CM-CD pathway encompasses extracellular starch-active cyclomaltodextrin glucanotransferases (CGTases) to synthesize linear dextrins and CDs, it is the ABC importer system in the second step that is the critical factor in determining which molecules from the CGTase activity will be internalized by the cell. Here, structure-function relationship studies of the cyclo⁄maltodextrin-binding protein MdxE of the MdxEFG-MsmX importer system from Thermoanaerobacter mathranii subsp. mathranii A3 are presented. Calorimetric and fluorescence studies of recombinant MdxE using linear dextrins and CDs showed that although MdxE binds linear dextrins and CDs with high affinity, the open-to-closed conformational change is solely observed after α- and β-CD binding, suggesting that the CM-CD pathway from Thermoanaerobacterales is exclusive for cellular internalization of these molecules. Structural analysis of MdxE coupled with docking simulations showed an overall architecture typically found in sugar-binding proteins (SBPs) that comprised two N- and C-domains linked by three small hinge regions, including the conserved aromatic triad Tyr193/Trp269/Trp378 in the C-domain and Phe87 in the N-domain involved in CD recognition and stabilization. Structural bioinformatic analysis of the entire MdxFG-MsmX importer system provided further insights into the binding, internalization, and delivery mechanisms of CDs. Hence, while the MdxE-CD complex couples to the permease subunits MdxFG to deliver the CD into the transmembrane channel, the dimerization of the cytoplasmatic promiscuous ATPase MsmX triggers active transport into the cytoplasm. This research provides the first results on a novel thermofunctional SBP and its role in the internalization of CDs in extremely thermophilic bacteria.

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Section: Methodsmentioning

confidence: 99%

Binding Specificity of a Novel Cyclo/Maltodextrin-Binding Protein and Its Role in the Cyclodextrin ABC Importer System from Thermoanaerobacterales

Aranda-Caraballo,

Saenz,

López-Zavala

et al. 2023

Molecules

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“…Special attention is being paid to the characterization of amaranth proteins. Almost all publications emphasize their high biological value, as determined by the balance of the amino acid composition, which significantly exceeds that of grain crops [ 3 , 4 , 5 ]. However, analyzing the data presented in the works of various research centers reveals that the amino acid score (determined by the ratio of essential amino acids and their specific content) varies in a fairly wide range, from 80% to 100% [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Sidorova

Петров

Перова

et al. 2023

Foods

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Special attention is being paid to the study of amaranth proteins. They are characterized by a high biological value that significantly exceeds those of grain crops. The production of protein concentrate from amaranth flour includes preliminary enzymatic hydrolysis, extraction of the resulting mixture, protein precipitation, microfiltration, and freeze-drying. In our study, the obtained amaranth protein concentrate was limited by valine, with an amino acid score of 74%. The true digestibility of the amaranth protein concentrate determined in vivo was 97.6 ± 0.3%, which was significantly lower than that of casein (99.3 ± 0.2%). The protein digestibility-corrected amino acid score value of the concentrate was 72.2%. The obtained concentrate was a rich source of selenium, copper, magnesium, manganese, and iron. Ferulic acid was the only polyphenolic compound found in the amaranth protein concentrate, but its content was significantly greater compared to the original flour. Saponins were not removed completely during the process of obtaining the amaranth protein concentrate. We identified 15 saponins in the concentrate, mainly of the bidesmoside type, the sapogenins of which are related derivatives of oleanolic acid. Thus, the developed amaranth protein concentrate can be used as an ingredient in functional food products, with a high biological value.

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Amaranth proteins: From extraction to application as nanoparticle-based delivery systems for bioactive compounds

Hadidi,

Aghababaei,

Mahfouzi

et al. 2024

Food Chemistry

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Characterization of the Technofunctional Properties and Three-Dimensional Structure Prediction of 11S Globulins from Amaranth (Amaranthus hypochondriacus L.) Seeds

Cited by 3 publications

References 46 publications

Binding Specificity of a Novel Cyclo/Maltodextrin-Binding Protein and Its Role in the Cyclodextrin ABC Importer System from Thermoanaerobacterales

Binding Specificity of a Novel Cyclo/Maltodextrin-Binding Protein and Its Role in the Cyclodextrin ABC Importer System from Thermoanaerobacterales

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Amaranth proteins: From extraction to application as nanoparticle-based delivery systems for bioactive compounds

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