Characterization of the subsite structure of the β-glucosidase from Aspergillus niger, an aspect of the mechanism of carbohydrate recognition11Presented in part at the Annual Meeting of the Japan Society for Glycoscience (Oyo Toshitsu-kagaku), Tsukuba, Japan 1996.

Ohnishi, Masatake; Okada, Gentaro; Yazaki, Terutaka

doi:10.1016/s0008-6215(98)00025-1

Cited by 12 publications

(1 citation statement)

References 18 publications

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“…Due to the high MoS of the investigated polymers, only a small fraction of the respective polymer will fulfill the spatial requirements of the enzyme and be degraded, especially if the polymer is homogeneously substituted. The study was accomplished by comparing HPC samples from two different suppliers using several different endoglucanases in combination with β-glucosidase, which hydrolyses terminal nonreducing β- d -glucose residues releasing d -glucose. , The latter was used to raise the glucose content to a level where quantification with high pH anion exchange chromatography with pulsed amperometric detection (HPAEC−PAD) is easier and gives results that are more reliable. SEC-MALS−RI was used to determine the average molar mass before and after enzymatic degradation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Chemical Substitution of Hydroxypropyl Cellulose Using Enzymatic Degradation

et al. 2005

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The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade a series of hydroxypropyl cellulose (HPC) derivatives with a high degree of substitution. The HPCs were characterized with cloud-point analysis prior to degradation. The extent of enzymatic degradation was determined with size-exclusion chromatography with online multi-angle light scattering and refractive index detection and also with high-pH anion exchange chromatography with pulsed amperometric detection. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained oligosaccharides. The different endoglucanases showed varying degradation capability depending on structure of the active site. The highly substituted HPCs had different susceptibility to degradation by the endoglucanases. The results show a difference in substituent distribution between HPCs, which would explain the differing cloud-point behaviors. Increased number of regions with low substitution could be correlated with lower polymer cloud point. The study shows the usefulness of enzymatic degradation to study the distribution of substituents in soluble biopolymer derivates.

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Section: Introductionmentioning

confidence: 99%

Characterization of Chemical Substitution of Hydroxypropyl Cellulose Using Enzymatic Degradation

et al. 2005

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ChemInform Abstract: Characterization of the Subsite Structure of the β‐Glucosidase from Aspergillus niger, an Aspect of the Mechanism of Carbohydrate Recognition.

Ohnishi¹,

Okada²,

Yazaki³

1998

ChemInform

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The influence of malolactic fermentation and Oenococcus oeni strain on glycosidic aroma precursors and related volatile compounds of red wine

Ugliano

Moio

2006

J Sci Food Agric

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Free and glycosidically bound volatile compounds of red wine were measured after malolactic fermentation (MLF) with four different commercial starter cultures of Oenococcus oeni. MLF resulted in a significant decrease in the concentration of total glycosides, expressed as phenol-free glycosyl glucose. Gas chromatographic analyses of wine enzyme hydrolysates showed that the extent of hydrolysis of glycosides during MLF was dependent on both bacterial strain and chemical structure of the substrate. The highest decrease was observed for glycosidic precursors of primary terpene alcohols. Glycoside-related aroma compounds such as linalool, farnesol, and β-damascenone were increased after MLF with all the bacterial strains tested. Two of the strains were also able to release significant amounts of vinylphenols during MLF.

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Characterization of the subsite structure of the β-glucosidase from Aspergillus niger, an aspect of the mechanism of carbohydrate recognition11Presented in part at the Annual Meeting of the Japan Society for Glycoscience (Oyo Toshitsu-kagaku), Tsukuba, Japan 1996.

Cited by 12 publications

References 18 publications

Characterization of Chemical Substitution of Hydroxypropyl Cellulose Using Enzymatic Degradation

Characterization of Chemical Substitution of Hydroxypropyl Cellulose Using Enzymatic Degradation

ChemInform Abstract: Characterization of the Subsite Structure of the β‐Glucosidase from Aspergillus niger, an Aspect of the Mechanism of Carbohydrate Recognition.

The influence of malolactic fermentation and Oenococcus oeni strain on glycosidic aroma precursors and related volatile compounds of red wine

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