Characterization of the sub-transition of hydrated dipalmitoylphosphatidylcholine bilayers. Kinetic, hydration and structural study

Ruocco, Martin J.; Shipley, G. Graham

doi:10.1016/0005-2736(82)90420-5

Cited by 332 publications

(191 citation statements)

References 17 publications

Supporting

Mentioning

163

Contrasting

Unclassified

Order By: Relevance

“…2b) at T = 14.1 o C shows reflection at q = 1.49 ± 0.002Å -1 and a broad shoulder at q = 1.53 ± 0.01 Å -1 . These reflections correspond to an orthorhombic subcell with the lattice constants 8.46 ± 0.01Å and 9.38± 0.07Å [11]. The two reflections separate monotonously as the temperature decreases to the temperature of ice formation in the positions 1.46 ± 0.002Å -1 and 1.58 ± 0.002Å -1 (lattice constants 8.61 ± 0.01Å and 8.97 ± 0.08Å).…”

Section: Resultsmentioning

confidence: 95%

“…Figure 2a presents the time resolved diffraction from a multilamellar structure of a DPPC membrane. At cooling from 14.1°C to −16.2°C the diffraction patterns correspond to the transformation of the gel phase in to the crystalline phase [11]. The shift of the diffraction peak in the direction of a larger scattering vector q at T ice = −19.4°C corresponds to a decrease of the membrane repeat distance d. It is the result of ice formation in a bulk solvent.…”

Section: Resultsmentioning

confidence: 96%

“…Slow progressive conversion of interchain space is the result of membrane crystallization. The membrane cystallization is a time and temperature dependent process with a characteristic time of 12 hours [11]. The description of such process is beyond of the scope of this article.…”

Section: Resultsmentioning

confidence: 99%

“…The slope of curves correspond to the first stages of membrane crystallization. The membrane crystallization is accompanied with the dehydration of intermembrane space, for this type of dehydration the characteristic time is 12 hours which corresponds to slow water diffusion from intermembrane space to bulk solvent [11]. In the case of iceinduced dehydration, water diffusion from intermembrane space to bulk solvent actually occurs in no time, the characteristic time is ≤ 3min.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Ice formation in model biological membranes in the presence of cryoprotectors

Киселев

Lesieur

Kisselev

et al. 2000

Nuclear Instruments and Methods in Physics Research Section A:

View full text Add to dashboard Cite

Ice formation in model biological membranes is studied by SAXS and WAXS in the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as well-studied model biological membranes. A special cryostat is used for sample cooling from 14.1 o C to −55.4 o C. The ice formation is only detected by WAXS in binary phospholipid/water and ternary phospholipid/cryoprotector/water systems in the condition of excess solvent. Ice formation in a binary phospholipid/water system creates an abrupt decrease of the membrane repeat distance by ∆d, so-called ice-induced dehydration of intermembrane space. The value of ∆d decreases as the cryoprotector concentration increases. The formation of ice does not influence the membrane structure (∆d = 0) for cryoprotector mole fractions higher than 0.05. IntroductionDimethyl sulfoxide (DMSO) and glycerol are well-known cryoprotectors widely used in cryobiology for the conservation of biological tissues at low temperatures [1]. The main purpose of cryobiology is to find an "optimal" way for cooling biological systems to low temperatures (about the liquid nitrogen temperature), and prevent the crystallisation of water inside biological tissues. One of the problems is a longer time of freeze-induced dehydration (diffusion of water molecules from biological tissues through the membrane to the conserving solvent) than the time of ice formation [2]. The ice-induced dehydration of intermembrane space at the moment of water crystallization is observed by X-ray diffraction [3] and calorimetry [4] for binary phospholipid /water systems. In macromolecular crystallography the portion of X-ray diffraction experiments at cryogenic temperatures is exponentially increasing. In many cases, a dramatic reduction in radiation damage at low temperatures allows complete data sets to be collected from a single crystal. cryoprotectors make it possible to cool samples to low temperature and store them without damage in the crystal [5].In this article ice formation is investigated by small-and wide-angle X-ray scattering (SAXS and WAXS) in ternary phospholipid/cryoprotector/water systems. Three phospholipids are studied: dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and distearoylphosphatidylcholine (DSPC). DPPC, DMPC, and DSPC are taken as phospholipids with an equal polar heads and with the difference in the length of hydrocarbon chains. The reason why we use DMSO and glycerol is the fact of their different interactions with the membrane surface. Glycerol penetrates into the region of polar head groups and produces influence on the thickness of the membrane bilayer and the thickness of an intermembrane solvent [6]. DMSO molecules do not penetrate into the region of polar head groups and thus do not influence the membrane thickness [7,8]. DMSO molecules strongly interact with water molecules and form hydrogen bonds. Such interaction is more intensive than the interaction between DMSO a...

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Ice formation in model biological membranes in the presence of cryoprotectors

Киселев

Lesieur

Kisselev

et al. 2000

Nuclear Instruments and Methods in Physics Research Section A:

View full text Add to dashboard Cite

show abstract

“…[13][14][15][16][17] The aromatic backbone length of the newly designed molecules is 3.2 nm and is thus in the range of the typical thickness of the lipophilic part of a phospholipid bilayer. [18][19] The total length between the ends of the glycerol units is, depending on the assumed conformation, in the range from 4.0 to 4.4 nm (see Fig 1). This molecule combines the concepts of rigidity and flexibility, and moieties with different philicities, i.e., the core being lipophilic, rigid and amenable to - stacking interactions, the side chains being lipophilic but flexible, and the head groups being hydrophilic and capable of hydrogen-bonding.…”

Section: Introductionmentioning

confidence: 99%

Temperature-Dependent In-Plane Structure Formation of an X-Shaped Bolapolyphile within Lipid Bilayers

et al. 2015

View full text Add to dashboard Cite

The polyphilic compound B12 is an X-shaped molecule with stiff aromatic core, flexible aliphatic side chains and hydrophilic end groups. Forming a thermotropic triangular honeycomb phase in the bulk between 177 °C and 182 °C, but no lyotropic phases, it is designed to fit into DPPC or DMPC lipid bilayers, in which it phase separates at room temperature, as observed in giant unilamellar vesicles (GUVs) by fluorescence microscopy.

show abstract

Structural Phase Transition due to a Release of Bound Water in Phospholipid Bilayers at Temperatures below 0°C

Grünert

Börngen

Nimtz

1984

Ber Bunsenges Phys Chem

View full text Add to dashboard Cite

Biophysical Chemistry / Liquid Crystals / Membranes / Phase TransitionsMeasurements of dielectric and calorimetric properties and of the lamellar repeat distance revealed a structural phase transition at temperatures below 0°C in hydrated phospholipid (DPPC) bilayers. The transition is marked 1. by a jump of the dielectric constant at microwave frequencies, 2. by a jump of the lamellar repeat distance, and 3. by an enthalpy of transition. These observations are interpretated by a change of the amount of bound water. Below the transition temperature only seven H,O molecules per lipid molecule are left bound as interlamellar water independent on the amount bound at higher temperatures. The observed loss of bound water at this subzero temperature phase transition may represent a physical process for irreversible freezing effects.Ber. Bunsenges. Phys. Chem. 88, 608-612 (1984) -0 Verlag Chemie GmbH, D-6940 Weinheim, 1984.

show abstract

Characterization of the sub-transition of hydrated dipalmitoylphosphatidylcholine bilayers. Kinetic, hydration and structural study

Cited by 332 publications

References 17 publications

Ice formation in model biological membranes in the presence of cryoprotectors

Ice formation in model biological membranes in the presence of cryoprotectors

Temperature-Dependent In-Plane Structure Formation of an X-Shaped Bolapolyphile within Lipid Bilayers

Structural Phase Transition due to a Release of Bound Water in Phospholipid Bilayers at Temperatures below 0°C

Contact Info

Product

Resources

About