Characterization of the Stiffness of Multiple Particles Trapped by Dielectrophoretic Tweezers in a Microfluidic Device

Son, Min Ho; Choi, Seungyeop; Ko, Kwan Hwi; Kim, Min Hyung; Lee, Sei Young; Key, Joe L.; Yoon, Young Joon; Park, In Soo; Lee, Sang Woo

doi:10.1021/acs.langmuir.5b03677

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…It can be concluded that these movements of the trapped particles were from the thermal fluctuations (from Brownian motion) and have no significant impact on the capability of particle trapping within tested conditions [32,53,56,57]. These results show that the DEP trap force acting on the particle was also increased by an increase in the potential of the applied electric field, while the trapped particle remained close to the surface of the traps.…”

Section: Resultsmentioning

confidence: 93%

“…For the quantitative analysis of these movements of the trapped particles, experimental results described in Figs. 6 and 7 were used to compute DEP trap stiffness for the circular‐shape trap using the equipartition theorem [32]. The equipartition theorem is the simplest and straightforward method to determine trap stiffness from thermal fluctuations of a trapped particle in the trapped position [53,56–58].…”

Section: Resultsmentioning

confidence: 99%

“…Over the years, trapping force spectroscopy and trap stiffness have been investigated for various DEP trapping. DEP trap stiffness and force spectroscopy were analyzed for particle trapping based on interdigitated electrodes [10,31,32]. It has been reported that the devices with interdigitated electrodes suffer from particle levitation.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

et al. 2021

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Dielectrophoresis is a robust approach for the manipulation and separation of (bio)particles using microfluidic platforms. We developed a dielectrophoretic corral trap in a microfluidic device that utilizes negative dielectrophoresis to capture single spherical polystyrene particles. Circular‐shaped micron‐size traps were employed inside the device and the three‐dimensional trap stiffness (restoring trapping force from equilibrium trapping location) was analyzed using 4.42 μm particles and 1 MHz of an alternating electric field from 6 VP‐P to 10 VP‐P. The trap stiffness increased exponentially in the x‐ and y‐direction, and linearly in the z‐direction. Image analysis of the trapped particle movements revealed that the trap stiffness is increased 608.4, 539.3, and 79.7% by increasing the voltage from 6 VP‐P to 10 VP‐P in the x‐, y‐, and z‐direction, respectively. The trap stiffness calculated from a finite element simulation of the device confirmed the experimental results. This analysis provides important insights to predict the trapping location, strength of the trapping, and optimum geometry for single particle trapping and its applications such as single‐molecule analysis and drug discovery.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

et al. 2021

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“…For the second approach, we modified the grayscale measurement method used to evaluate the time-dependent grayscale values corresponding to the upward behavior of micro-objects [ 37 , 38 ] to measure the lateral behavior of a cell, as follows. (1) During the experiment, we defined two different circular windows in the chip.…”

Section: Methodsmentioning

confidence: 99%

Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device

Choi

Lim

et al. 2018

Sensors

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Characterization of cellular dielectrophoretic (DEP) behaviors, when cells are exposed to an alternating current (AC) electric field of varying frequency, is fundamentally important to many applications using dielectrophoresis. However, to date, that characterization has been performed with monotonically increasing or decreasing frequency, not with successive increases and decreases, even though cells might behave differently with those frequency modulations due to the nonlinear cellular electrodynamic responses reported in previous works. In this report, we present a method to trace the behaviors of numerous cells simultaneously at the single-cell level in a simple, robust manner using dielectrophoretic tweezers-based force spectroscopy. Using this method, the behaviors of more than 150 cells were traced in a single environment at the same time, while a modulated DEP force acted upon them, resulting in characterization of nonlinear DEP cellular behaviors and generation of different cross-over frequencies in living cells by modulating the DEP force. This study demonstrated that living cells can have non-linear di-polarized responses depending on the modulation direction of the applied frequency as well as providing a simple and reliable platform from which to measure a cellular cross-over frequency and characterize its nonlinear property.

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“…There are many important techniques used for trapping or manipulating these objects in various fields of science. These are dielectrophoretic tweezer [1], acoustic tweezer [2], magnetic tweezer [3], hydrodynamic trapping with microfluidic valving [4], optical tweezer and laser trapping [5]. Each of these techniques is suitable for different research areas and has its particular advantages or drawbacks that determine proper applications.…”

Section: Optical Tweezer System With No Fluorescent Confocal Microscomentioning

confidence: 99%

Optical tweezer system with no fluorescent confocal microscope for trapping colloidal nanoparticles

Nuansri¹,

Buranasiri²,

Limsuwan³

et al. 2018

Ukr. J. Phys. Opt.

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We describe two optical tweezer systems for the studies of laser trapping of fluorescent colloidal nanoparticles (NPs). The first one, conventional optical tweezer system widely used in laser trapping, requires a fluorescent confocal microscope for observing trapped NPs. The second system, with no microscope, is presented for the first time in this work. The quantity of trapped NPs for this system is estimated from the transmitted laser light intensity that passes through the fluorescent colloidal NPs. Then the transmitted laser light is converted into the voltage signal and measured by an oscilloscope. A small capillary tube to be filled by the colloidal NPs is developed and used in the second system. This tube can be used with light-sensitive cameras for which a danger of damaging by high light intensities exists. Finally, we show that the results obtained using the both tweezer systems are in good agreement.

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Characterization of the Stiffness of Multiple Particles Trapped by Dielectrophoretic Tweezers in a Microfluidic Device

Cited by 11 publications

References 28 publications

Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

Quantitative analysis of the three‐dimensional trap stiffness of a dielectrophoretic corral trap

Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device

Optical tweezer system with no fluorescent confocal microscope for trapping colloidal nanoparticles

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