Characterization of the stemness and osteogenic potential of oral and sinus mucosal cells

Chen, Ming Hsu; Tai, Wei‐Chiu; Cheng, Nai‐Chen; Chang, Chien‐Wen; Chang, Po-Chun

doi:10.1016/j.jfma.2021.06.017

Cited by 3 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro cell culture was conducted according to previous studies (Chen et al, 2022; Gruber et al, 2004; Srouji et al, 2010) with some improvement (Figure S1). Under sterile conditions and with a stereomicroscope, ophthalmic forceps were used to gently isolate the remaining bone wall fragments with the adherent periosteum layer, so that the lamina propria was fully exposed.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, MSCs located in the human maxillary sinus membrane (hMSM) were discovered (Srouji et al, 2009). Further studies confirmed that MSCs derived from hMSM could be cultured in vitro and induced to differentiate towards osteogenesis (Berbéri et al, 2017(Berbéri et al, , 2021Chen et al, 2022;Cho et al, 2014;Graziano et al, 2012;Guo et al, 2015;Kim et al, 2009;Ren et al, 2022;Srouji et al, 2009Srouji et al, , 2010. The isolation and analyses of MSCs from hMSM could be helpful in clarifying the osteogenic mechanism of hMSM and designing targeted therapeutic strategies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Lv,

Xu,

Wang

et al. 2024

J Clinic Periodontology

View full text Add to dashboard Cite

AimTo discover the populations of mesenchymal stem cells (MSCs) derived from different layers of human maxillary sinus membrane (hMSM) and evaluate their osteogenic capability.Materials and MethodshMSM was isolated into a monolayer using the combined method of physical separation and enzymatic digestion. The localization of MSCs in hMSM was performed by immunohistological staining and other techniques. Lamina propria layer‐derived MSCs (LMSCs) and periosteum layer‐derived MSCs (PMSCs) from hMSM were expanded using the explant cell culture method and identified by multilineage differentiation assays, colony formation assay, flow cytometry and so on. The biological characteristics of LMSCs and PMSCs were compared using RNA sequencing, reverse transcription and quantitative polymerase chain reaction, immunofluorescence staining, transwell assay, western blotting and so forth.ResultsLMSCs and PMSCs from hMSMs were both CD73‐, CD90‐ and CD105‐positive, and CD34‐, CD45‐ and HLA‐DR‐negative. LMSCs and PMSCs were identified as CD171+/CD90+ and CD171−/CD90+, respectively. LMSCs displayed stronger proliferation capability than PMSCs, and PMSCs presented stronger osteogenic differentiation capability than LMSCs. Moreover, PMSCs could recruit and promote osteogenic differentiation of LMSCs.ConclusionsThis study identified and isolated two different types of MSCs from hMSMs. Both MSCs served as good potential candidates for bone regeneration.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Lv,

Xu,

Wang

et al. 2024

J Clinic Periodontology

View full text Add to dashboard Cite

show abstract

“…A recent investigation reported that alveolar mucosal cells (AMCs) contained a high proportion of stem cells and showed superior osteogenic potential relative to the attached gingival cells. 19 As alveolar mucosa revealed faster healing potential than attached gingiva 20 and alveolar mucosa harvesting did not alter the masticatory mucosa and thus had minimal influence on the periodontal maintenance, 21 AMCs might be better alternative MSCs for facilitating wound healing and bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…demonstrated that the keratinization of oral mucosal epithelium depended on the stimuli from the underlying connective tissue, 18 suggesting the behaviors and populations of cells in the lamina propria of alveolar mucosa and attached gingiva might be different. A recent investigation reported that alveolar mucosal cells (AMCs) contained a high proportion of stem cells and showed superior osteogenic potential relative to the attached gingival cells 19 . As alveolar mucosa revealed faster healing potential than attached gingiva 20 and alveolar mucosa harvesting did not alter the masticatory mucosa and thus had minimal influence on the periodontal maintenance, 21 AMCs might be better alternative MSCs for facilitating wound healing and bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Alveolar mucosal cell spheroids promote extraction socket healing and osseous defect regeneration

Zheng

Cheng

et al. 2023

Journal of Periodontology

View full text Add to dashboard Cite

Background:Alveolar mucosa could be a promising source of mesenchymal stem cells (MSCs) for regeneration therapeutics because it exhibits faster healing potential and can be easily collected with minimal periodontal disturbance. This study aimed to evaluate the potential of alveolar mucosal cell (AMC) spheroids for promoting extraction socket healing and calvarial osseous defect regeneration.Methods:AMCs were isolated from Sprague‐Dawley rats. Antigenic and mesenchymal stem cell (MSC) surface markers expressions and trilineage differentiation capability were assessed. AMCs were then osteogenically simulated (OAs) or unstimulated (UAs), self‐aggregated to form spheroids, and encapsulated in the gelatin hydrogel, to fill rat extraction sockets or combined with freeze‐dried bone graft (FDBG) to fill rat calvarial osseous defects. The outcome was assessed by gross observation, micro‐CT imaging, and immunohistochemistry.Results:AMCs highly expressed MSC surface markers, showed weak antigenicity, and were capable of trilineage differentiation at passage 3. In extraction sockets, wound closure, socket fill, keratinization, and proliferative activities, were accelerated in those with AMC spheroids. Socket fill and maturation was further promoted by OA spheroids. In calvarial osseous defects, the mineralized tissue ratio was promoted with AMC spheroids/FDBG treatment, bone sialoprotein expression and cell proliferation were more evident with OA spheroids/FDBG treatment.Conclusion:AMCs exhibited MSC properties with weak antigenicity. AMC spheroids promoted extraction socket healing, AMC spheroids/FDBG promoted calvarial osseous defect regeneration, and the outcomes were further enhanced by osteogenically stimulation of AMCs.This article is protected by copyright. All rights reserved

show abstract

Highlights

Kao¹

2022

Journal of the Formosan Medical Association

View full text Add to dashboard Cite

Characterization of the stemness and osteogenic potential of oral and sinus mucosal cells

Cited by 3 publications

References 35 publications

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Isolation, identification and osteogenic capability analysis of mesenchymal stem cells derived from different layers of human maxillary sinus membrane

Alveolar mucosal cell spheroids promote extraction socket healing and osseous defect regeneration

Highlights

Contact Info

Product

Resources

About