Characterization of the soybean gene<i>Gm</i>ENOD40-2

Roussis, Andreas; Sande, K. van de; Papadopoulou, Kalliopi; Drenth, Janneke; Bisseling, Ton; Franssen, Henk; Katinakis, Panagiotis

doi:10.1093/jxb/46.6.719

Cited by 12 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A soybean GmENOD40-2 promoter-gus fusion has been introduced into a heterologous plant, vetch, and found to be expressed only in the pericycle of the nodule vascular bundle and at the region of the root where the nodule was attached (Roussis et al, 1995). However, the spatial and temporal expression patterns of the second soybean ENOD40 gene in a transgenic plant have not yet been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…1), that have been identified in several nodulin gene promoters (Sandal et al, 1987;Bak Ramlov et al, 1993;Miao and Verma, 1993;Roussis et al, 1995). Using deletion analysis and site-specific mutagenesis, these sequences have been found to be required for nodule-specific expression for certain genes, such as lbc3 and nodulin-26 (Stougaard et al, 1990;Bak Ramlov et al, 1993;Miao and Verma, 1993;Szcyzglowski et al, 1994).…”

Section: Locationmentioning

confidence: 99%

See 1 more Smart Citation

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

1998

View full text Add to dashboard Cite

ENOD40, an early nodulin gene, is expressed following inoculation with Rhizobium meliloti or by adding R. meliloti-produced nodulation (Nod) factors or the plant hormone cytokinin to uninoculated roots. We isolated two MsENOD40 clones, designated MsENOD40-1 and MsENOD40-2, with distinct promoters from an alfalfa (Medicago sativa cv Chief) genomic library. The promoters were fused to the reporter gene uidA (gus), and the constructs were introduced into alfalfa. We observed that the MsENOD40-1 construct was expressed almost exclusively under symbiotic conditions. The MsENOD40-2 construct was transcribed under both symbiotic and nonsymbiotic conditions and in nonnodular and nodular tissues. Both MsENOD40 promoter-gus constructs were similarly expressed as nodules developed, and both were expressed in roots treated with 6-benzylaminopurine or purified Nod factor. However, no blue color was detected in nodule-like structures induced by the auxin transport inhibitor N-1-(naphthyl)phthalamic acid on roots of plants containing the MsENOD40-1 promoter construct, whereas pseudonodules from plants containing the MsENOD40-2 promoter construct stained blue. A 616-bp region at the distal 5 end of the promoter is important for proper spatial expression of MsENOD40 in nodules and also for Nod-factor and cytokinin-induced expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Locationmentioning

confidence: 99%

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

1998

View full text Add to dashboard Cite

show abstract

“…When using the same approach with the promoter from another early nodulin gene from soybean, GmEnod40-2 (Roussis et al, 1995), we previously reported that the construct GmEnod40-2-gus was not expressed either during the early stages of the symbiotic interaction with Frankia (Santi et al, 2003b). So far, there are two symbiotic promoters that retain the same pattern of expression in both Legume and actinorhizal root nodule symbioses.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Expression Pattern Conferred by the PsEnod12B Promoter from the Early Nodulin Gene of Pisum sativum in Transgenic Actinorhizal Trees of the Casuarinaceae Family

Constans

Obertello

et al. 2006

Plant Soil

View full text Add to dashboard Cite

To investigate the similarities between nitrogen-fixing symbioses in Rhizobium-legumes and Frankia-actinorhizal plants, we studied the gus expression pattern conferred by the early nodulin promoter PsEnod12B from Pisum sativum in two transgenic Casuarinaceae trees, Allocasuarina verticillata and Casuarina glauca. Respectively 6 and 13 transgenic lines of C. glauca and A. verticillata were obtained following genetic transformation with the disarmed strain of Agrobacterium tumefaciens C58C1 (pGV2260; pBIN-PsEnod12B-gus). In non-symbiotic tissues, the gus gene was expressed exclusively in the vascular system of the aerial parts of the plants. In mature nodules, PsEnod12B drove some gus expression in large Frankiainfected cortical cells. This specificity was different from that observed in pea nodules where PsEnod12B was found expressed in the infection zone. No reporter gene activity was visible at the early stages of the symbiotic process with Frankia, including infected root hairs and prenodules. Whereas some transcription factors of Allocasuarina and Casuarina are able to interact with the PsEnod12B promoter, the pattern of expression is modified in nodules of these non-legume heterologous hosts.Abbreviations: BA -6-benzylaminopurine; GUS -b-glucuronidase; NAA -a naphtalene acetic acid; X-gluc -5-bromo-4-chloro-3-indolyl b-D-glucuronic acid.

show abstract

“…The subclones were sequenced and the sequences were assembled leading to a contig of 15,000 bp. Comparison of the genomic LeENOD40 sequence (gLeENOD40; data not shown) and the cDNA sequence (cLeENOD40) revealed that the ENOD40 gene in tomato does not contain introns, as is the case for all other studied ENOD40 genes (Roussis et al 1995;Fang and Hirsch 1998;Flemetakis et al 2000).…”

Section: Isolation Of the Leenod40 Genementioning

confidence: 91%

Expression of ENOD40 during tomato plant development

Vleghels

Hontelez

Ribeiro

et al. 2003

Planta

Self Cite

View full text Add to dashboard Cite

In legumes, ENOD40 expression is increased upon interaction of plants with rhizobia. Little is known of the expression pattern of ENOD40 during other stages of the plant life cycle. Studies of ENOD40 expression in non-legume development may give an indication of the function of the gene. To investigate the ENOD40 expression pattern during plant development, a fusion between the beta-glucuronidase (GUS) reporter gene and 150 bp of the 5' untranslated region plus 3,000 bp of 5' untranscribed tomato ENOD40 sequence was constructed and introduced into Lycopersicon esculentum Miller. Based on the observed GUS expression patterns in transgenic tomato we speculate that ENOD40 in tomato has a role in counteracting ethylene-provoked responses.

show abstract

Characterization of the soybean geneGmENOD40-2

Cited by 12 publications

References 23 publications

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

Analysis of the Expression Pattern Conferred by the PsEnod12B Promoter from the Early Nodulin Gene of Pisum sativum in Transgenic Actinorhizal Trees of the Casuarinaceae Family

Expression of ENOD40 during tomato plant development

Contact Info

Product

Resources

About