Characterization of the secretome of Plasmopara viticola by de novo transcriptome analysis

Yin, Ling; Li, Xinlong; Jiang, Xiang; Qu, Junjie; Zhang, Yali; Dry, Ian B.; Lu, Jiang

doi:10.1016/j.pmpp.2015.05.002

Cited by 39 publications

(59 citation statements)

References 64 publications

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“…Different numbers of effectors were also predicted from the two Chinese isolates and one Australian isolate in our previous transcriptome study40. This phenomenon is the result of pathogen adaptation to different grapevine genotypes.…”

Section: Discussionmentioning

confidence: 66%

“…The presence of the conserved RXLR motif makes it feasible to identify candidate RXLR effector genes within the genome sequence of oomycete pathogens39. A total of 100 putative RXLR-dEER like effectors (Supplementary Table S7) were predicted from the secretome of the Chinese P. viticola isolate ‘JL-7-2’, and 31 of which had previously been identified by de novo assembly of transcriptome data from three P. viticola isolates infecting on grapevine leaves40. Mestre et al 41.…”

Section: Resultsmentioning

confidence: 99%

“…However, previous analysis of oomycete CRN proteins has shown that CRN signal peptides are not always readily detected by SignalP analysis2745. Using the mining method described by Yin et al 40,. a total of 90 putative PvCRN proteins were identified from the P. viticola genome (Supplementary Table S9).…”

Section: Resultsmentioning

confidence: 99%

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Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

Yin

et al. 2017

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Plasmopara viticola causes downy mildew disease of grapevine which is one of the most devastating diseases of viticulture worldwide. Here we report a 101.3 Mb whole genome sequence of P. viticola isolate ‘JL-7-2’ obtained by a combination of Illumina and PacBio sequencing technologies. The P. viticola genome contains 17,014 putative protein-coding genes and has ~26% repetitive sequences. A total of 1,301 putative secreted proteins, including 100 putative RXLR effectors and 90 CRN effectors were identified in this genome. In the secretome, 261 potential pathogenicity genes and 95 carbohydrate-active enzymes were predicted. Transcriptional analysis revealed that most of the RXLR effectors, pathogenicity genes and carbohydrate-active enzymes were significantly up-regulated during infection. Comparative genomic analysis revealed that P. viticola evolved independently from the Arabidopsis downy mildew pathogen Hyaloperonospora arabidopsidis. The availability of the P. viticola genome provides a valuable resource not only for comparative genomic analysis and evolutionary studies among oomycetes, but also enhance our knowledge on the mechanism of interactions between this biotrophic pathogen and its host.

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Section: Discussionmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

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Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

Yin

et al. 2017

Sci Rep

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“…However, to our knowledge, no senso stricto avirulence function for a CRN effector has been shown. P. viticola RXLR expression patterns peaked as early as 48 h post-infection, probably because of the direct leaf disk dipping method used for grape downy mildew inoculation (Mestre et al, 2012; Yin et al, 2015). …”

Section: Discussionmentioning

confidence: 99%

RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines

et al. 2016

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Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances.

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“…Fiftyfour ESTs encoding potential secreted hydrolytic enzymes and effectors were identified from germinated zoospores of P. viticola (As-sadi et al, 2011;Cabral et al, 2011;Mestre et al, 2012). Based on the presence of a secretory signal sequence and an RxLR or CRN motif, 51 RxLR effectors and 10 CRN effectors were identified from P. viticola (Yin et al, 2015). RNA-based sequencing has been used to identify RxLR/CRN genes that are differentially expressed upon infection of grapevine (Brilli et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Insight Into Function and Subcellular Localization of Plasmopara viticola Putative RxLR Effectors

et al. 2020

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Grapevine downy mildew, caused by oomycete fungus Plasmopara viticola, is one of the most devastating diseases of grapes across the major production regions of the world. Although many putative effector molecules have been identified from this pathogen, the functions of the majority of these are still unknown. In this study, we analyzed the potential function of 26 P. viticola effectors from the highly virulent strain YL. Using transient expression in leaf cells of the tobacco Nicotiana benthamiana, we found that the majority of the effectors could suppress cell death triggered by BAX and INF1, while seven could induce cell death. The subcellular localization of effectors in N. benthamiana was consistent with their localization in cells of Vitis vinifera. Those effectors that localized to the nucleus (17/26) showed a variety of subnuclear localization. Ten of the effectors localized predominantly to the nucleolus, whereas the remaining seven localized to nucleoplasm. Interestingly, five of the effectors were strongly related in sequence and showed identical subcellular localization, but had different functions in N. benthamiana leaves and expression patterns in grapevine in response to P. viticola. This study highlights the potential functional diversity of P. viticola effectors.

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Characterization of the secretome of Plasmopara viticola by de novo transcriptome analysis

Cited by 39 publications

References 64 publications

Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines

Insight Into Function and Subcellular Localization of Plasmopara viticola Putative RxLR Effectors

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