Characterization of the Salmonella typhimurium pagC/pagD chromosomal region

Gunn, John S.; Alpuche-Aranda, Celia; Loomis, Wendy P.; Belden, William J.; Miller, Samuel I.

doi:10.1128/jb.177.17.5040-5047.1995

Cited by 112 publications

(81 citation statements)

References 42 publications

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“…The spaN and sipB genes were shown to have a role in entry into non-phagocytic cells and killing of macrophages [37]. Additionally, there are genes required for the survival within macrophages, including pagC [6], msgA [38], and spiA [39]. Others have a role in iron acquisition, including sitC [40] and iroN [41].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt

Tarabees

El-Sayed

Shawish

et al. 2017

J Infect Dev Ctries

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Introduction: Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection. Methodology: In this study, 100 raw chicken meat samples were investigated for the presence of Salmonella spp., which were subsequently identified based on biochemical and serological tests as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) profile. Furthermore, the isolated serovars were examined using multiplex polymerase chain reaction (PCR) for the presence of virulence genes suspected to have a role in infection. Results: S. Enteritidis was isolated from two samples (2%), while S. Typhimurium was isolated from three samples (3%) of chicken meat. Of the 17 examined virulence genes using multiplex PCR, the sitC, sopB, sifA, lpfC, spaN, sipB, invA, spiA, and msgA genes were detected in S. Enteritidis. However, the sitC, iroN, sopB, sifA, lpfC, spaN, sipB, invA, and tolC genes were successfully amplified in S. Typhimurium. Conclusions: The detection of S. Enteritidis and S. Typhimurium in meat, even at low incidence, has important implications. In addition, the data presented here is the first attempt to identify a wide range of virulence genes in Egyptian Salmonella isolates recovered from meat products. A strict public health and food safety regime is urgently needed in order to decrease the human health hazard risk associated with salmonellosis.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt

Tarabees

El-Sayed

Shawish

et al. 2017

J Infect Dev Ctries

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“…Fourteen genes-invA, orgA, prgH, spaN (invJ ), tolC, sipB, pagC, msgA, spiA, sopB, lpfC, pefA, spvB, and sifA-assayed in this study encode products that are associated with cellular invasion, survival within a cell, and adhesin or pili production (2, 3,4,5,7,8,12,16,18,19,26,27,29). The invA, prgH, spaN (invJ ), sipB, spiA, and sifA genes are also associated with type III secretion systems (TTSSs) (18,28,30), complex structures of more than 20 proteins that are used in protein delivery (10).…”

Section: Discussionmentioning

confidence: 99%

“…Other targeted genes encoded other traits thought to be important to virulence, including sitC (11) and iroN (1), both involved in iron acquisition, and cdtB, a putative toxin-encoding gene (9). All of the genes except pefA, iroN, cdtB, sipB, and spaN (invJ) have been shown to be required for full Salmonella virulence in a murine model (2, 3,5,7,8,11,12,16,18,20,26,27).…”

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confidence: 99%

“…isolated from birds with salmonellosis were different from the Salmonella isolates from healthy birds by their invasiveness in cell culture (13,17). Therefore, in an effort to genetically differentiate between Salmonella from sick and healthy birds, the majority of the genes targeted in this study-invA, orgA, prgH, spaN (invJ), tolC, sipB, pagC, msgA, spiA, sopB, lpfC, pefA, spvB, and sifA-encode proteins associated with invasiveness traits, such cellular invasion/survival and adhesin or pili production (2, 3,4,5,7,8,12,16,18,20,26,27,28). Other targeted genes encoded other traits thought to be important to virulence, including sitC (11) and iroN (1), both involved in iron acquisition, and cdtB, a putative toxin-encoding gene (9).…”

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confidence: 99%

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Virulence Genotyping of Salmonella spp. with Multiplex PCR

Skyberg¹,

Logue

Nolan³

2006

Avian Diseases

156

135

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The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located onSalmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative control strains was verified, the genotypes of 78 Salmonella isolates incriminated in avian salmonellosis (primarily from sick, commercially reared chickens and turkeys) and 80 Salmonella isolates from apparently healthy chickens or turkeys were compared. Eleven of the 17 genes tested (invA, orgA, prgH, tolC, spaN [invJ], sipB, sitC, pagC, msgA, spiA, and iroN) were found in all of the isolates. Another (sopB) was present in all isolates from sick birds and all but one isolate from healthy birds. The remaining five genes (lpfC, cdtB, sifA, pefA, and spvB) were found in 10%-90% of the isolates from sick birds and 3.75%-90% of the healthy birds. No significant differences in the occurrence of these genes between the two groups of isolates were detected. These results suggest that these virulence genes, and presumably the PAIs and TTSSs with which they are associated, are widely distributed among Salmonella isolates of birds, regardless of whether their hosts of origin have been identified as having salmonellosis. SUMMARY. The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located on Salmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative control strains was verified, the genotypes of 78 Salmonella isolates incriminated in avian salmonellosis (primarily from sick, commercially reared chickens and turkeys) and 80 Salmonella isolates from apparently healthy chickens or turkeys were compared. Eleven of the 17 genes tested (invA, orgA, prgH, tolC, spaN [invJ], sipB, sitC, pagC, msgA, spiA, and iroN) were found in all of the isolates. Another (sopB) was present in all isolates from sick birds and all but one isolate from healthy birds. The remaining five genes (lpfC, cdtB, sifA, pefA, and spvB) were found in 10%-90% of the isolates from sick birds and 3.75%-90...

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“…The PhoP-activated genes most dependent on the MgtA protein specify proteins of unknown function and, like the mgtA gene, are not required for virulence in an animal (19)(20)(21). Therefore, we hoped that the investigation of mgtA-dependent phenotypes might reveal a novel aspect(s) of Salmonella's lifestyle.…”

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confidence: 99%

Flagella-independent surface motility in Salmonella enterica serovar Typhimurium

Park

Pontes

Groisman

2015

Proc. Natl. Acad. Sci. U.S.A.

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Flagella are multiprotein complexes necessary for swimming and swarming motility. In Salmonella enterica serovar Typhimurium, flagella-mediated motility is repressed by the PhoP/PhoQ regulatory system. We now report that Salmonella can move on 0.3% agarose media in a flagella-independent manner when experiencing the PhoP/PhoQ-inducing signal low Mg 2+ . This motility requires the PhoP-activated mgtA, mgtC, and pagM genes, which specify a Mg 2+ transporter, an inhibitor of Salmonella's own F 1 F o ATPase, and a small protein of unknown function, respectively. The MgtA and MgtC proteins are necessary for pagM expression because pagM mRNA levels were lower in mgtA and mgtC mutants than in wild-type Salmonella, and also because pagM expression from a heterologous promoter rescued motility in mgtA and mgtC mutants. PagM promotes group motility by a surface protein(s), as a pagM-expressing strain conferred motility upon a pagM null mutant, and proteinase K treatment eliminated motility. The pagM gene is rarely found outside subspecies I of S. enterica and often present in nonfunctional allelic forms in organisms lacking the identified motility. Deletion of the pagM gene reduced bacterial replication on 0.3% agarose low Mg 2+ media but not in low Mg 2+ liquid media. Our findings define a form of motility that allows Salmonella to scavenge nutrients and to escape toxic compounds in low Mg 2+ semisolid environments.

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Characterization of the Salmonella typhimurium pagC/pagD chromosomal region

Cited by 112 publications

References 42 publications

Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt

Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt

Virulence Genotyping of Salmonella spp. with Multiplex PCR

Flagella-independent surface motility in Salmonella enterica serovar Typhimurium

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