Characterization of the role of global regulator FliA in the pathophysiology of Pseudomonas aeruginosa infection

Lo, Yi-Ling; Chen, Chyi‐Liang; Shen, Lunda; Chen, Ying-Ching; Wang, Yi-Hsin; Lee, Chung-Chan; Wang, Lian-Chen; Chuang, Chih-Hsien; Chiu, Cheng-Hsun; Chiu, Cheng‐Hsun; Chang, Hwan‐You

doi:10.1016/j.resmic.2018.02.001

Cited by 10 publications

(14 citation statements)

References 42 publications

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“…2). The RpoN sigma factor is known to be involved inter alia in the transcription of genes necessary for nitrogen assimilation and motility [56,57], whereas the FliA factor activates genes required for flagellin biosynthesis and adherence [18,58,59].…”

Section: Comparative Analysis Of Gene Expression Of Pao1 In Uninfectementioning

confidence: 99%

Introducing differential RNA-seq mapping to track the early infection phase forPseudomonasphage ɸKZ

et al. 2020

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As part of the ongoing renaissance of phage biology, more phage genomes are becoming available through DNA sequencing. However, our understanding of the transcriptome architecture that allows these genomes to be expressed during host infection is generally poor. Transcription start sites (TSSs) and operons have been mapped for very few phages, and an annotated global RNA map of a phagealone or together with its infected host-is not available at all. Here, we applied differential RNA-seq (dRNA-seq) to study the early, host takeover phase of infection by assessing the transcriptome structure of Pseudomonas aeruginosa jumbo phage ɸKZ, a model phage for viral genetics and structural research. This map substantially expands the number of early expressed viral genes, defining TSSs that are active ten minutes after ɸKZ infection. Simultaneously, we record gene expression changes in the host transcriptome during this critical metabolism conversion. In addition to previously reported upregulation of genes associated with amino acid metabolism, we observe strong activation of genes with functions in biofilm formation (cdrAB) and iron storage (bfrB), as well as an activation of the antitoxin ParD. Conversely, ɸKZ infection rapidly down-regulates complexes IV and V of oxidative phosphorylation (atpCDGHF and cyoABCDE). Taken together, our data provide new insights into the transcriptional organization and infection process of the giant bacteriophage ɸKZ and adds a framework for the genome-wide transcriptomic analysis of phage-host interactions.

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Section: Comparative Analysis Of Gene Expression Of Pao1 In Uninfectementioning

confidence: 99%

Introducing differential RNA-seq mapping to track the early infection phase forPseudomonasphage ɸKZ

et al. 2020

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“…As described in the Introduction, FliA‐family sigma factors are involved in a wide variety of bacterial behaviors in addition to flagellar biogenesis. It is worth noting that a fliA ‐overexpressing strain displayed several phenotypes similar to those of a fliA ‐defective strain in P. aeruginosa , suggesting that the optimum intracellular level of FliA is critical to its proper function (Lo et al, ). In this respect, it is interesting that a putative FliA‐dependent promoter sequence exists in the region upstream from fliA1 (Figure a).…”

Section: Discussionmentioning

confidence: 99%

“…In E. coli , FliA modulates the intracellular concentration of cyclic dimeric GMP (c‐di‐GMP) (Claret et al, ). Furthermore, FliA in P. aeruginosa is a global transcriptional regulator controlling multiple gene networks for adherence to and invasion of mammalian cells, interbacterial competition and phenazine pigment production (Lo et al, , ). The comprehensive regulon of FliA was identified in E. coli using chromatin immunoprecipitation followed by the next‐generation sequencing and RNA sequencing (RNA‐Seq) techniques, indicating that the sigma factor directly regulates at least 14 transcriptional units under the tested conditions (Fitzgerald, Bonocora, & Wade, ).…”

Section: Introductionmentioning

confidence: 99%

Involvement of three FliA‐family sigma factors in the sporangium formation, spore dormancy and sporangium dehiscence in Actinoplanes missouriensis

Hashiguchi

Tezuka

Ohnishi

2020

Molecular Microbiology

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The rare actinomycete Actinoplanes missouriensis forms sporangia, which open up and release zoospores in response to water. Here, we report a genetic and functional analysis of four FliA‐family sigma factors, FliA1, FliA2, FliA3 and FliA4. Transcription of fliA1, fliA2 and fliA3 was directly activated by the global transcriptional activator TcrA during sporangium formation and dehiscence, while fliA4 was almost always transcribed at low levels. Gene disruption analysis showed that (a) deletion of fliA2 reduced the zoospore swimming speed by half, (b) the fliA1‐fliA2 double‐deletion mutant formed abnormal sporangia in which mutant spores ectopically germinated and (c) deletion of fliA3 induced no phenotypic changes in the wild‐type and mutant strains of fliA1 and/or fliA2. Comparative RNA‐Seq analyses among the wild‐type and gene deletion mutant strains showed probable targets of each FliA‐family sigma factor, indicating that FliA1‐ and FliA2‐dependent promoters are quite similar to each other, while the FliA3‐dependent promoter is somewhat different. Gene complementation experiments also indicated that the FliA1 regulon overlaps with the FliA2 regulon. These results demonstrate that A. missouriensis has developed a complex transcriptional regulatory network involving multiple FliA‐family sigma factors for the accomplishment of its characteristic reproduction process, including sporangium formation, spore dormancy and sporangium dehiscence.

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“…Accumulating evidence indicates that flagella are an important structure for pathogenic bacteria [37,38,39]. Prior to this work, fliA had been described as an essential gene for flagellar synthesis and pathogenesis [19,20]; therefore, it is necessary to explore the role of fliA in the host-pathogen interaction of P. plecoglossicida .…”

Section: Discussionmentioning

confidence: 99%

“…σ 28 has been documented to regulate flagellar synthesis in Escherichia coli [14], Campylobacter jejuni [15], Pseudomonas aeruginosa [16], Salmonella typhimurium [17] and Vibrio cholera [18]. Furthermore, knocking out fliA in P. aeruginosa resulted in reduced motility, with decreased colonization in the intestines of mice because flagella were not synthesized [19]. In addition, the fliA mutant strain of Legionella pneumophila exhibits reduced motility, weakened biofilm, reduced macrophage infectivity and decreased colonization potential in host cells [20].…”

Section: Introductionmentioning

confidence: 99%

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

et al. 2019

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In the present study, Larimichthys crocea and Pseudomonas plecoglossicida were selected as a host-pathogen interaction model for teleosts and prokaryotic pathogens. Five shRNAs were designed and synthesized to silence the fliA gene, all of which resulted in pronounced reductions in fliA mRNA; the mutant strain with the best silencing efficiency of 92.16% was chosen for subsequent analysis. A significant decrease in motility, intracellular survival and escape was observed for the fliA-RNAi strain of P. plecoglossicida, whereby silencing of the fliA gene led to a 30% decrease in mortality and a four-day delay in the onset of infection in L. crocea. Moreover, silencing of P. plecoglossicida fliA resulted in a significant change in both the pathogen and host transcriptome in the spleens of infected L. crocea. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of pathogen transcriptome data showed that silencing fliA resulted in downregulation of 18 flagellum-related genes; KEGG analysis of host transcriptome data revealed that infection with the fliA-RNAi strain caused upregulation of 47 and downregulation of 106 immune-related genes. These pathogen-host interactions might facilitate clearance of P. plecoglossicida by L. crocea, with a significant decrease in fliA-RNAi P. plecoglossicida strain virulence in L. crocea.

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Characterization of the role of global regulator FliA in the pathophysiology of Pseudomonas aeruginosa infection

Cited by 10 publications

References 42 publications

Introducing differential RNA-seq mapping to track the early infection phase forPseudomonasphage ɸKZ

Introducing differential RNA-seq mapping to track the early infection phase forPseudomonasphage ɸKZ

Involvement of three FliA‐family sigma factors in the sporangium formation, spore dormancy and sporangium dehiscence in Actinoplanes missouriensis

Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

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