Characterization of the role of RILP in cell migration

Margiotta, Azzurra; Progida, Cinzia; Bakke, Oddmund; Bucci, Cecilia

doi:10.4081/ejh.2017.2783

Cited by 12 publications

(8 citation statements)

References 37 publications

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“…Rab7 can also interact with and increase the activity of rac1, a small GTPase protein that acts as a central regulator of actin remodeling. [50][51][52] Increased rac1 activity has been associated with an increase in the rate of macropinocytosis 52 and a decrease in clathrinmediated uptake of molecules such as epidermal growth factor and transferring. 53 Accordingly, we hypothesized that the Br-EV-driven decrease in rab7 can indirectly affect the rate of EV endocytosis.…”

Section: Br-evs Downregulate Endothelial Rab7 To Facilitate Their Tramentioning

confidence: 99%

Tumor-Derived Extracellular Vesicles Breach the Intact Blood–Brain Barrier via Transcytosis

Carman²,

et al. 2019

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Author Contributions G.M. designed and performed the experiments, analyzed and interpreted the data, and wrote the manuscript. M.A.M. designed and supervised the research, analyzed and interpreted the data, and wrote the manuscript. C.V.C. co-designed and assisted with high spatiotemporal resolution microscopy experiments. E.J.H. and J.R.P. co-designed and assisted with zebrafish experiments. N.M. and T.P. co-designed and performed the BBB-on-a-chip experiments. C.C.D. assisted with some of the experiments. L.I.Z. and D.E.I. provided zebrafish and BBB-on-a-chip models, respectively.

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Section: Br-evs Downregulate Endothelial Rab7 To Facilitate Their Tramentioning

confidence: 99%

Tumor-Derived Extracellular Vesicles Breach the Intact Blood–Brain Barrier via Transcytosis

Carman²,

et al. 2019

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“…In the present work, miR-16b decreased the expression of rilp, atxn7, tnika, btbd9, indicating these genes may contribute to miR-16b function. rilp has regulatory roles in cell motility affecting migration velocity, cell adhesion and cell spreading [50]. atxn7 encodes a transcription factor related to chromatin remodeling [51].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells

Jiang

Luo

et al. 2020

PLoS ONE

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MicroRNAs (miRNAs) play vital roles in various biological processes under multiple stress conditions by leading to mRNA cleavage or translational repression. However, the detailed roles of miRNAs in cold acclimation in fish are still unclear. In the present study, highthroughput sequencing was performed to identify miRNAs from 6 small RNA libraries from the zebrafish embryonic fibroblast ZF4 cells under control (28˚C, 30 days) and cold-acclimation (18˚C, 30 days) conditions. A total of 414 miRNAs, 349 known and 65 novel, were identified. Among those miRNAs, 24 (19 known and 5 novel) were up-regulated, and 23 (9 known and 14 novel) were down-regulated in cold acclimated cells. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses indicated that the target genes of known differentially expressed miRNAs (DE-miRNA) are involved in cold acclimation by regulation of phosphorylation, cell junction, intracellular signal transduction, ECM-receptor interaction and so on. Moreover, both miR-100-3p inhibitor and miR-16b mimics could protect ZF4 cells under cold stress, indicating the involvement of miRNA in cold acclimation. Further study showed that miR-100-3p and miR-16b could regulate inversely the expression of their target gene (atad5a, cyp2ae1, lamp1, rilp, atxn7, tnika, btbd9), and that overexpression of miR-100-3p disturbed the early embryonic development of zebrafish. In summary, the present data show that miRNAs are closely involved in cold acclimation in zebrafish ZF4 cells and provide information for further understanding of the roles of miRNAs in cold acclimation in fish.

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“…The directness (Equation (9)) is a measure of the straightness of the cell trajectories, which indicates varying degrees of cell polarity across the different cell phenotypes [ 40 ]. It is calculated by comparing the Euclidean distance (

) and the accumulated distance (

) between the starting point and the endpoint of a migrating cell.…”

Section: Methodsmentioning

confidence: 99%

Fibronectin-Expressing Mesenchymal Tumor Cells Promote Breast Cancer Metastasis

Jun

Guo

Libring

et al. 2020

Cancers

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Tumor metastasis is connected to epithelial-mesenchymal heterogeneity (EMH) and the extracellular matrix (ECM) within the tumor microenvironment. Mesenchymal-like fibronectin (FN) expressing tumor cells enhance metastasis within tumors that have EMH. However, the secondary tumors are primarily composed of the FN null population. Interestingly, during tumor cell dissemination, the invasive front has more mesenchymal-like characteristics, although the outgrowths of metastatic colonies consist of a more epithelial-like population of cells. We hypothesize that soluble FN provided by mesenchymal-like tumor cells plays a role in supporting the survival of the more epithelial-like tumor cells within the metastatic niche in a paracrine manner. Furthermore, due to a lower rate of proliferation, the mesenchymal-like tumor cells become a minority population within the metastatic niche. In this study, we utilized a multi-parametric cell-tracking algorithm and immunoblotting to evaluate the effect of EMH on the growth and invasion of an isogenic cell series within a 3D collagen network using a microfluidic platform. Using the MCF10A progression series, we demonstrated that co-culture with FN-expressing MCF10CA1h cells significantly enhanced the survival of the more epithelial MCF10CA1a cells, with a two-fold increase in the population after 5 days in co-culture, whereas the population of the MCF10CA1a cells began to decrease after 2.5 days when cultured alone (p < 0.001). However, co-culture did not significantly alter the rate of proliferation for the more mesenchymal MCF10CA1h cells. Epithelial tumor cells not only showed prolonged survival, but migrated significantly longer distances (350 µm compared with 150 µm, respectively, p < 0.01) and with greater velocity magnitude (4.5 µm/h compared with 2.1 µm/h, respectively, p < 0.001) under co-culture conditions and in response to exogenously administered FN. Genetic depletion of FN from the MCF10CA1h cells resulted in a loss of survival and migration capacity of the epithelial and mesenchymal populations. These data suggest that mesenchymal tumor cells may function to support the survival and outgrowth of more epithelial tumor cells within the metastatic niche and that inhibition of FN production may provide a valuable target for treating metastatic disease.

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Characterization of the role of RILP in cell migration

Cited by 12 publications

References 37 publications

Tumor-Derived Extracellular Vesicles Breach the Intact Blood–Brain Barrier via Transcytosis

Tumor-Derived Extracellular Vesicles Breach the Intact Blood–Brain Barrier via Transcytosis

Identification and characterization of miRNAs involved in cold acclimation of zebrafish ZF4 cells

Fibronectin-Expressing Mesenchymal Tumor Cells Promote Breast Cancer Metastasis

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