Characterization of the Replication of Plasmids Containing hr Sequences in Baculovirus-Infected Spodoptera frugiperda Cells

Leisy, Douglas J.; Rohrmann, George F.

doi:10.1006/viro.1993.1529

Cited by 95 publications

(98 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For quantitative measurements, samples were diluted to obtain juvenile hormone to juvenile hormone acid conversion rates between 10 and 50%, and activities were calculated on the basis of the dilution factors. (20), treated with RNase-free DNase, and reverse-transcribed using as primer oligo(dT) [12][13][14][15][16][17][18] . Aliquots of cDNA reactions were subjected to semiquantitative PCR amplification using cat gene and chorion gene-specific primers as described previously (20).…”

Section: Cloning Of the Hr3 Element And Construction Of Expression Plasmentioning

confidence: 99%

A Baculovirus (Bombyx mori Nuclear Polyhedrosis Virus) Repeat Element Functions as a Powerful Constitutive Enhancer in Transfected Insect Cells

Farrell

Johnson

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

It has been previously reported that baculovirus homologous regions, the regions of baculovirus genomes that contain the origins of DNA replication, can augment the expression of a small number of baculovirus genes in vitro. We are now reporting that a region of the genome of Bombyx mori nuclear polyhedrosis virus (Bm-NPV) containing the homologous region 3 (HR3) acts as an enhancer for the promoter of a nonviral gene, the cytoplasmic actin gene of the silkmoth B. mori. Incorporation of the HR3 sequences of BmNPV into an actin promoter-based expression cassette results in an augmentation of transgene expression in transfected cells by two orders of magnitude relative to the control recombinant expression cassette. This increase is due to a corresponding increase in the rate of transcription from the actin promoter and not to replication of the expression cassette and occurs only when the HR3 element is linked to the expression cassette in cis. A comparable degree of enhancement in the activity of the silkworm actin promoter occurs also in heterologous lepidopteran cells. Concomitant supplementation of transfected cells with the BmIE1 trans-activator, which was previously shown to be capable of functioning in vitro as a transcriptional co-activator of the cytoplasmic actin gene promoter, results in more than a 1,000-fold increase in the level of expression of recombinant proteins placed under the control of the actin gene promoter. These findings provide the foundation for the development of a nonlytic insect cell expression system for continuous high-level expression of recombinant proteins. Such a system should provide levels of expression of recombinant proteins comparable to those obtained from baculovirus expression systems and should also have the additional advantage of continuous production in a cellular environment that, in contrast to that generated by a baculovirus infection, supports continuously proper posttranslational modifications of recombinant proteins and the capability of expression of proteins from genomic as well as cDNA sequences.

show abstract

Section: Cloning Of the Hr3 Element And Construction Of Expression Plasmentioning

confidence: 99%

A Baculovirus (Bombyx mori Nuclear Polyhedrosis Virus) Repeat Element Functions as a Powerful Constitutive Enhancer in Transfected Insect Cells

Farrell

Johnson

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The most well-characterized baculovirus, Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV), has a circular dsDNA genome of 134 kb, which encodes approximately 150 genes (Ayres et al, 1994). It has eight homologous regions (hrs) which have been shown to act as early gene transcriptional enhancers (Guarino & Summers, 1986 ;Leisy et al, 1995 ;Rodems & Friesen, 1993 and may function as origins of DNA replication (Leisy et al, 1995 ;Leisy & Rohrmann, 1993 ;Pearson et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).

Evans

Rosenblatt

Leisy

et al. 1999

Journal of General Virology

View full text Add to dashboard Cite

LEF-3 and P143 are two of six proteins encoded by the Autographa californica multinucleocapsid nucleopolyhedrovirus genome which are required for DNA replication in transient replication assays. LEF-3 has the properties of an ssDNA-binding protein and P143 exhibits amino acid sequence homology to helicases. In this report, the interaction of LEF-3 and P143 was studied by yeast two-hybrid and immunoaffinity analyses. Using the yeast two-hybrid system, the interaction domain of LEF-3 (385 aa) was mapped to amino acids between positions 1 and 165. Deletion analysis of P143 failed to reveal an interaction domain, suggesting that there were either multiple interaction domains or that the deletions disrupted the secondary structures required for the interaction between LEF-3 and P143.

show abstract

“…hrs function as enhancers of RNA polymerase II-mediated transcription of baculovirus early promoters (Guarino & Summers, 1986;Theilmann & Stewart, 1992). In both AcMNPV and OpMNPV, hrs can perform as cis-acting origins of DNA replication Leisy & Rohrmann, 1993;Kool et al, 1995;Pearson et al, 1992); it has also been suggested that hrs can probably substitute for each other, since it was recently shown that not all hrs need to be present for successful DNA replication (Carstens &Wu, 2007). In seven of the 33 baculoviruses currently sequenced [ChchNPV, Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV), Agrotis segetum granulovirus (AgseGV), CpGV, Neodiprion abietis nucleopolyhedrovirus (NeabNPV) and Neodiprion lecontii nucleopolyhedrovirus (NeleNPV)] canonical hrs were not identified.…”

Section: Sequence Analysis Of Mavinpv Genomementioning

confidence: 99%

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Chen

Lee

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C content, and contained 126 open reading frames (ORFs) encoding predicted proteins of over 50 aa. The gene content and gene order of MaviNPV have the highest similarity to those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome with fewer auxiliary genes than the AcMNPV type species. Except for one ORF (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is the first lepidopteranspecific baculovirus to lack homologues of vfgf and odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the MaviNPV genome, and these contained a total of 44 imperfect palindromes. Phylogenetic analysis of the whole genome revealed that MaviNPV was separated from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before these two viral species diverged from each other. Moreover, replication of MaviNPV in several cell lines and an egfp-MaviNPV infection assay revealed that IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.

show abstract

Characterization of the Replication of Plasmids Containing hr Sequences in Baculovirus-Infected Spodoptera frugiperda Cells

Cited by 95 publications

References 0 publications

A Baculovirus (Bombyx mori Nuclear Polyhedrosis Virus) Repeat Element Functions as a Powerful Constitutive Enhancer in Transfected Insect Cells

A Baculovirus (Bombyx mori Nuclear Polyhedrosis Virus) Repeat Element Functions as a Powerful Constitutive Enhancer in Transfected Insect Cells

Characterization of the interaction between the baculovirus ssDNA-binding protein (LEF-3) and putative helicase (P143).

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Contact Info

Product

Resources

About