CHARACTERIZATION OF THE RED PIGMENT FORMED IN THE 2‐THIOBARBITURIC ACID DETERMINATION OF OXIDATIVE RANCIDITY<sup>a,</sup><sup>b</sup>

Sinnhuber, R. O.; Yu, T. C.

doi:10.1111/j.1365-2621.1958.tb17614.x

Cited by 388 publications

(136 citation statements)

References 10 publications

(1 reference statement)

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“…Ascorbic acid content was estimated using thiourea reagent and 2,4-dinitrophenylhydrazine (11). Lipid peroxidation was estimated using Trichloroacetic acid (TCA)-Thiobarbituric acid (TBA)-Hydrochloric acid (HCl) at 535 nm (12). The reduced glutathione (GSH) content of the tissue in 0.1M phosphate buffer (pH 7.4) was measured following the method of Ellman (13) using DTNB reagent [5,5'-dithiobis (2-nitrobenzoic acid)].…”

Section: Biochemical Methodsmentioning

confidence: 99%

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Das

Vasudevan

2006

Indian J Clin Biochem

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Alcoholic liver disease (ALD) develops as a consequence of priming and sensitizing mechanisms rendered by cross-interactions of primary mechanistic factors and secondary risk factors. Liver damage due to consumption of alcohol may be caused by oxygen radicals such as superoxide and hydroxyl radicals, generated during the metabolism of ethanol by the microsomal oxidizing system. Lecithin, an important class of phospholipids contains choline, which is considered as lipotropic factor. The effects of this lecithin as a hepatoprotective drug on body weight and antioxidant status of ethanol-exposed rats were studied. The results were compared with the effects of tocopheryl acetate. From the present study, it can be concluded that ethanol-induced stress can be partly prevented by tocopheryl acetate, and showed best result. Abstination from alcohol also involved for little hepatic regeneration. Supplementation of lecithin showed better effect compared to abstination from alcohol on reversing the effect of ethanol induced liver damage in the present study. Moreover, preventive measures were found to be better than curative treatment. Antioxidants are likely to provide beneficial effects on hepatocyes via desensitization against oxidant stress while inhibiting primary mechanism for expression of proinflammatory and cytotoxic mediators. However, abstinence from alcohol, proper nutrition, and supplementation of antioxidants, vitamins and hepatoprotective drugs are some of the therapeutic options.

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Section: Biochemical Methodsmentioning

confidence: 99%

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Das

Vasudevan

2006

Indian J Clin Biochem

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“…The oxidizing agent H 2 O 2 (500 lM) and/or the grape extract (10 mg) from skin or pulp of each cultivar were added and incubated at 37°C with mild shaking for 1 h. Appropriate controls were also set up. After incubation, the goat liver slices were homogenized in the incubation medium and each the homogenate was used for assaying protein [13], thiobarbituric acid reactive substances (TBARS) [20], nitrite [21], reduced glutathione (GSH) [22], as well as activities of catalase (EC 1.11.1.6) [23], glutathione peroxidase (GPx; EC 1.11.1.9) [24], GR (EC 1.6.4.2) [25] and glutathione S-transferase (GST; EC 2.5.1.18) [26].…”

Section: Ex Vivo Studiesmentioning

confidence: 99%

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Singha

Das

2014

Ind J Clin Biochem

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Grapes are the richest source of antioxidants due to the presence of potent bioactive phytochemicals. In this study, the phytochemical contents, scavenging activities and protective role against H 2 O 2 -induced oxidative stress in liver tissue ex vivo of four grape (Vitis vinifera) cultivars extracts, namely Flame seedless (black), Kishmish chorni (black with reddish brown), Red globe (red) and Thompson seedless mutant (green), were evaluated. The total phenolics and flavonoids content in pulp or skin fractions of different grape cultivars were in the range of 47.6-310 mg gallic acid equivalent/g fresh weight (fw), and 46.6-733.3 lg catechin equivalent/g fw respectively. The scavenging activities in skin of different grape varieties against 2,2-diphenyl-1-picrylhydrazyl (44-58 %), hydrogen peroxide (15.3-18.6 %), and hydroxyl radicals (50-85 %), were higher than pulp of the corresponding cultivars. These scavenging activities of grape extracts were found to be significantly (p \ 0.01) correlated with the levels of total phenols, flavonoids and ascorbic acid. Liver tissues from goat treated with H 2 O 2 (500 lM) showed significantly decreased GSH content by 42.9 % and activities of catalase by 50 % and glutathione reductase by 66.6 %; while increased thiobarbituric acid reactive substances and nitric oxide level by 2.53-and 0.86-fold, respectively, and activity of glutathione S-transferase by 0.96-fold. Grape skin extracts showed the stronger protective activity against H 2 O 2 -induced oxidative stress in liver tissue ex vivo, than its pulp of any cultivar; and the Flame seedless (black) cultivar showed the highest potential. In conclusion, our study suggested that the higher antioxidant potential, phytochemical contents and significant scavenging capacities in pulp and skin of grape extracts showed the protective action of grape extracts against H 2 O 2 -induced oxidative stress in liver tissue ex vivo.

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“…Determination of plasma thiobarbituric-acid-reactive substances (TBARS): The extent of lipid peroxidation was determined by assaying malondialdehyde (MDA) formation according to the method of Sinnhuber et al (4). To 0.5 ml of the serum, 0.5 ml of 30% trichloro acetic acid (TCA) and 100…”

Section: Methodsmentioning

confidence: 99%

Association of plasma level of thiobarbituric acid reactive substances with extent of hepatocellular injury in preterm infants with cholestatic jaundice

Bhutia

Upadhyay

Maneesh

2006

Indian J Clin Biochem

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The present study was designed to determine the association between extent of hepatocellular injury and plasma level of thiobarbituric acid reactive substances (TBARS) SGOT (F=276.92; P<0.0001) and SGPT (F=355.17; P<0.0001) and differed significantly between cholestatic and control infants. Our findings suggest that oxidative stress in preterm infants with cholestasis is associated with hepatocellular injury.

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CHARACTERIZATION OF THE RED PIGMENT FORMED IN THE 2‐THIOBARBITURIC ACID DETERMINATION OF OXIDATIVE RANCIDITY^a,^b

Cited by 388 publications

References 10 publications

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Association of plasma level of thiobarbituric acid reactive substances with extent of hepatocellular injury in preterm infants with cholestatic jaundice

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CHARACTERIZATION OF THE RED PIGMENT FORMED IN THE 2‐THIOBARBITURIC ACID DETERMINATION OF OXIDATIVE RANCIDITYa,b

Cited by 388 publications

References 10 publications

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Effect of lecithin in the treatment of ethanol mediated free radical induced hepatotoxicity

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Association of plasma level of thiobarbituric acid reactive substances with extent of hepatocellular injury in preterm infants with cholestatic jaundice

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CHARACTERIZATION OF THE RED PIGMENT FORMED IN THE 2‐THIOBARBITURIC ACID DETERMINATION OF OXIDATIVE RANCIDITY^a,^b