Characterization of the pumpkin Translationally-Controlled Tumor Protein<i>CmTCTP</i>

Hinojosa-Moya, Jesús; Xoconostle‐Cázares, Beatriz; Toscano-Morales, Roberto; Ramírez-Ortega, Francisco Arturo; Cabrera-Ponce, José Luis; Ruíz-Medrano, Roberto

doi:10.4161/psb.26477

Cited by 16 publications

(29 citation statements)

References 37 publications

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“…Overall, in Arabidopsis, the levels of AtTCTP1 transcript and protein are correlated (Berkowitz et al, 2008 ), although low protein levels have been observed in inflorescence stem that did not correlate to the abundance of its mRNA, suggesting posttranscriptional or translational regulation (Brioudes et al, 2010 ). Similar results have been found for the Cucurbita maxima TCTP (CmTCTP); while elevated levels of the transcript are found in the shoot apex almost no protein is detected in this tissue (Hinojosa-Moya et al, 2013 ). Transcript and protein may also be regulated at the level of its cell-specific localization and intercellular movement; indeed, in some species TCTP transcript and protein are found in the phloem translocation stream, suggesting its long-distance movement (Lin et al, 2009 ; Rodríguez-Medina et al, 2011 ; Hinojosa-Moya et al, 2013 ).…”

Section: Introductionsupporting

confidence: 74%

“…Similar results have been found for the Cucurbita maxima TCTP (CmTCTP); while elevated levels of the transcript are found in the shoot apex almost no protein is detected in this tissue (Hinojosa-Moya et al, 2013 ). Transcript and protein may also be regulated at the level of its cell-specific localization and intercellular movement; indeed, in some species TCTP transcript and protein are found in the phloem translocation stream, suggesting its long-distance movement (Lin et al, 2009 ; Rodríguez-Medina et al, 2011 ; Hinojosa-Moya et al, 2013 ). TCTP appears to interact with the phloem RNA-binding protein CmPP16 (Aoki et al, 2005 ).…”

Section: Introductionsupporting

confidence: 74%

“…Transformation of tobacco leaf explants was carried out by the puncture method described by Hinojosa-Moya et al ( 2013 ). Basically, cultures containing A. rhizogenes K599 harboring the recombinant plasmids were grown on selective liquid media, concentrated, and resuspended in fresh media.…”

Section: Methodsmentioning

confidence: 99%

“…This is the case of Arabidopsis, which harbors two TCTP genes; one of them, AtTCTP1 (At3g16640), has a role in control of mitotic growth (Berkowitz et al, 2008 ; Brioudes et al, 2010 ); the other, AtTCTP2 (At3g05540), has been considered a pseudogene. Nonetheless, we have found that this gene is expressed and may be functional, since it can induce tobacco regeneration when harbored by Agrobacterium rhizogenes , in a manner analogous to CmTCTP , and that the mRNA was detected, albeit at much lower levels than the AtTCTP1 mRNA; also that the promoter is functional (Hinojosa-Moya et al, 2013 ; Toscano-Morales et al, submitted). In the present report we took advantage of the capacity of AtTCTP2 to induce whole plant regeneration in tobacco to obtain transgenic tobacco plants harboring a 35S::AtTCTP2-GFP construct.…”

Section: Introductionmentioning

confidence: 99%

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Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco

Toscano-Morales¹,

Xoconostle‐Cázares²,

Martínez-Navarro³

et al. 2014

Front. Plant Sci.

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Translationally Controlled Tumor Protein (TCTP) is an almost ubiquitous protein found in eukaryotes, fundamental for the regulation of development and general growth. The multiple functions of TCTP have been inferred from its involvement in several cell pathways, but the specific function of TCTP is still not known in detail. On the other hand, TCTP seems to respond to a plethora of external signals, and appears to be regulated at the transcriptional and/or translational levels by mechanisms yet to be determined. In the present work, we analyzed the capacity of AtTCTP2 gene products (mRNA and protein) to translocate long distance through tobacco heterografts (transgenic/WT and WT/transgenic). The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion). Interestingly, aerial roots emerged only in heterografts where the protein was detected in both stock and scion, suggesting a correlation between the presence of AtTCTP2 and aerial root appearance. More detailed analysis showed that these aerial roots harbored the transgene and expressed both transcript and protein. In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves. These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

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Section: Introductionsupporting

confidence: 74%

Section: Introductionsupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco

Toscano-Morales¹,

Xoconostle‐Cázares²,

Martínez-Navarro³

et al. 2014

Front. Plant Sci.

Self Cite

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show abstract

“…Its transcripts could be affected by diverse treatments, including drought, low temperature, high salt, ethylene, wounding, H 2 O 2 , and methyl jasmonate ). On the other hand, TCTP has been detected in the phloem sap implying its phloem long-distance movement in some plant species (Rodriguez-Medina et al 2011;Hinojosa-Moya et al 2013;Toscano-Morales et al 2014).…”

Section: Introductionmentioning

confidence: 99%

A translationally controlled tumor protein gene Rpf41 is required for the nodulation of Robinia pseudoacacia

et al. 2015

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Translationally controlled tumor protein (TCTP) is fundamental for the regulation of development and general growth in eukaryotes. Its multiple functions have been deduced from its involvement in several cell pathways, but its potential involvement in symbiotic nodulation of legumes cannot be suggested a priori. In the present work, we identified and characterized from the woody leguminous tree Robinia pseudoacacia a homolog of TCTP, Rpf41, which was up-regulated in the infected roots at 15 days post-inoculation but decreased in the matured nodules. Subcellular location assay showed that Rpf41 protein was located in the plasma membrane, cytoplasm, nucleus, and also maybe in cytoskeleton. Knockdown of Rpf41 via RNA interference (RNAi) resulted in the impaired development of both nodule and root hair. Compared with wild plants, the root and stem length, fresh weight and nodule number per plant was decreased dramatically in Rpf41 RNAi plants. The number of ITs or nodule primordia was also significantly reduced in the Rpf41 RNAi roots. The analyses of nodule ultrastructure showed that the infected cell development in Rpf41 RNAi nodules remained in zone II, which had fewer infected cells. Furthermore, the symbiosomes displayed noticeable shrinkage of bacteroid and peribacteroid space enlargement in the infected cells of Rpf41 RNAi nodules. In the deeper cell layers, a more remarkable aberration of the infected cell ultrastructure was observed, and electron-transparent lesions in the bacteroid cytoplasm were detected. These results identify TCTP as an important regulator of symbiotic nodulation in legume for the first time, and it may be involved in symbiotic cell differentiation and preventing premature aging of the young nodules in R. pseudoacacia.

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Translationally Controlled Tumor Protein and Its Relationship with Responses of Plants to Abiotic Stresses

Marques,

Barros,

de Souza

2023

Climate-Resilient Agriculture, Vol 2

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Characterization of the pumpkin Translationally-Controlled Tumor ProteinCmTCTP

Cited by 16 publications

References 37 publications

Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco

Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco

A translationally controlled tumor protein gene Rpf41 is required for the nodulation of Robinia pseudoacacia

Translationally Controlled Tumor Protein and Its Relationship with Responses of Plants to Abiotic Stresses

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