Characterization of the Propham Biodegradation Pathway in Starkeya sp. Strain YW6 and Cloning of a Novel Amidase Gene mmH

Abstract

We previously isolated a monocrotophos-degrading strain Starkeya sp. YW6, which could also degrade propham.Here, we show that strain YW6 metabolizes propham via a pathway in which propham is initially hydrolyzed to aniline and then converted to catechol, which is then oxidized via an ortho-cleavage pathway. The novel amidase gene mmH was cloned from strain YW6 and expressed in Escherichia coli BL21(DE3). MmH, which exhibits aryl acylamidase activity, was purified for enzymatic analysis. Bioinformatic analysis confirmed that MmH belongs to the amidase signature (AS) enzyme family and shares 26−50% identity with several AS family members. MmH (molecular mass of 53 kDa) was most active at 40 °C and pH 8.0 and showed high activity toward propham, with K cat and K m values of 33.4 s −1 and 16.9 μM, respectively. These characteristics make MmH suitable for novel amide biosynthesis and environmental remediation.

Biocatalytic Synthesis of Two Furan-Based Amino Compounds 2-Acetyl-4-aminofuran and 3-Acetylamino-5-(α-aminoethyl)-furan from Chitin Resources

“…As shown in Figure S5, the properties of MBP-MmH were consistent with those of MmH. 42 The optimum temperature and pH for MBP-MmH were 50 °C and 8.0, respectively. MBP-MmH was stable below 40 °C and at pH 7.0−10.0.…”

Functionally coherent transcriptional responses of Jatropha curcas and Pseudomonas fragi for rhizosphere mediated degradation of pyrene

“…3 E). Chen et al 45 had found that there is positive correlation between the increase activity of PPO due to higher PAH concentration in the soil 6 . Also in the present study, the residual pyrene concentration and PPO in untreated plants were higher than the DBC treated plants.…”

“…The United States Environmental Protection Agency (USEPA) has listed pyrene as one of the most abundant priority pollutants, among other 16 PAHs 5 . Pyrene is highly stable four ring compound and has been opted as a model compound to study the biodegradation of carcinogenic PAHs 6 . Pyrene is also known to negatively affect the growth of plants in contaminated soils.…”

Bacterioplankton Associated with Toxic Cyanobacteria Promote Pisum sativum (Pea) Growth and Nutritional Value through Positive Interactions

“…C12 were able to degrade 30% of 10 mg carbamazepine/L. Sun et al (2019) [ 85 ] described a metabolic pathway of Starkeya strains by which carbamate pesticides are degraded, which are esters of carbamic acid toxic to vertebrates and invertebrates because they inhibit the acetylcholinesterase (AChE) activity. In addition, the ability of Starkeya strains to destroy difficult-to-manage organic pollutants has been demonstrated, i.e., Naphthalene [ 86 ] and Polychlorinated biphenyls (PCBs) [ 87 ].…”