Characterization of the Polyamine Biosynthetic Pathways and Salt Stress Response in Brachypodium distachyon

Takahashi, Yoshihiro; Tahara, Misako; Yamada, Yuki; Mitsudomi, Yuka; Koga, Kaoruko

doi:10.1007/s00344-017-9761-z

Cited by 40 publications

(25 citation statements)

References 39 publications

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“…In higher plants, PAs are predominantly present in their free form. The most common PAs in higher plants are Put, Spd, Spm, thermospermine (Tspm) (Kim et al, 2014; Sobieszczuk-Nowicka, 2017; Takahashi et al, 2017b), and cadaverine (Cad) (Regla-Márquez et al, 2015; Nahar et al, 2016) (Table 1). Other PAs are found only in certain plants or under special conditions.…”

Section: Distribution and Metabolism Of Pas In Plantsmentioning

confidence: 99%

“…Polyamines show tissue- and organ-specific distribution patterns in plants. For example, the most abundant PA in leaves was found to be Put, and its levels were three times higher than those of Spd and Spm, whereas Spd was found to be the most abundant PA in other organs (Takahashi et al, 2017b). Different types of PAs also show different localization patterns within cells.…”

Section: Distribution and Metabolism Of Pas In Plantsmentioning

confidence: 99%

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Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

et al. 2019

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Polyamines (PAs) are low molecular weight aliphatic nitrogenous bases containing two or more amino groups. They are produced by organisms during metabolism and are present in almost all cells. Because they play important roles in diverse plant growth and developmental processes and in environmental stress responses, they are considered as a new kind of plant biostimulant. With the development of molecular biotechnology techniques, there is increasing evidence that PAs, whether applied exogenously or produced endogenously via genetic engineering, can positively affect plant growth, productivity, and stress tolerance. However, it is still not fully understood how PAs regulate plant growth and stress responses. In this review, we attempt to cover these information gaps and provide a comprehensive and critical assessment of the published literature on the relationships between PAs and plant flowering, embryo development, senescence, and responses to several (mainly abiotic) stresses. The aim of this review is to summarize how PAs improve plants' productivity, and to provide a basis for future research on the mechanism of action of PAs in plant growth and development. Future perspectives for PA research are also suggested.

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Section: Distribution and Metabolism Of Pas In Plantsmentioning

confidence: 99%

Section: Distribution and Metabolism Of Pas In Plantsmentioning

confidence: 99%

Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

et al. 2019

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“…Since halophytes have evolved physiological mechanisms to survive under natural saline conditions, these species could be considered as a potential reservoir for isolating salt tolerance genes. During the last decade, extensive efforts have been made to isolate and characterize salt tolerant genes from halophytes like smooth cordgrass ( Baisakh et al, 2008 ), Thellungiella salsuginea , a close relative of Arabidopsis ( Wu et al, 2012 ; Ellouzi et al, 2014 ), Salicornia europaea L. ( Lv et al, 2017 ), and Brachypodium distachyon ( Takahashi et al, 2017 ). Recently, transgenic rice plants overexpressing a soluble inorganic pyrophosphatase gene ThPP1 of Thellungiella halophila showed enhanced tolerance to alkaline stress compared to the wild type ( He et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Transgene Pyramiding of Salt Responsive Protein 3-1 (SaSRP3-1) and SaVHAc1 From Spartina alterniflora L. Enhances Salt Tolerance in Rice

2018

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The transgenic technology using a single gene has been widely used for crop improvement. But the transgenic pyramiding of multiple genes, a promising alternative especially for enhancing complexly inherited abiotic stress tolerance, has received little attention. Here, we developed and evaluated transgenic rice lines with a single Salt Responsive Protein 3-1 (SaSRP3-1) gene as well as pyramids with two-genes SaSRP3-1 and Vacuolar H+-ATPase subunit c1 (SaVHAc1) derived from a halophyte grass Spartina alterniflora L. for salt tolerance at seedling, vegetative, and reproductive stages. The overexpression of this novel gene SaSRP3-1 resulted in significantly better growth of E. coli with the recombinant plasmid under 600 mM NaCl stress condition compared with the control. During early seedling and vegetative stages, the single gene and pyramided transgenic rice plants showed enhanced tolerance to salt stress with minimal wilting and drying symptoms, improved shoot and root growth, and significantly higher chlorophyll content, relative water content, and K+/Na+ ratio than the control plants. The salt stress screening during reproductive stage revealed that the transgenic plants with single gene and pyramids had better grain filling, whereas the pyramided plants showed significantly higher grain yield and higher grain weight compared to control plants. Our study demonstrated transgenic pyramiding as a viable approach to achieve higher level of salt tolerance in crop plants.

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“…Studies have shown that polyamines exhibit tissue-and organ-specific distribution patterns in plants 14,33 . The most abundant polyamine in leaves is putrescine, and its level is three times higher than that of spermidine and spermine, whereas spermidine is the most abundant in other organs 33 . Different types of polyamines show different localisation patterns within cells.…”

Section: Discussionmentioning

confidence: 99%

Ectopic expression of GhSAMDC1 improved plant vegetative growth and early flowering through conversion of spermidine to spermine in tobacco

Zhu

Tian

Zhu

et al. 2020

Sci Rep

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Polyamines play essential roles in plant development and various stress responses. In this study, one of the cotton S-adenosylmethionine decarboxylase (SAMDC) genes, GhSAMDC 1 , was constructed in the pGWB17 vector and overexpressed in tobacco. Leaf area and plant height increased 25.9-36.6% and 15.0-27.0%, respectively, compared to the wild type, and flowering time was advanced by 5 days in transgenic tobacco lines. polyamine and gene expression analyses demonstrated that a decrease in spermidine and an increase in total polyamines and spermine might be regulated by NtSPDS 4 and NtSPMS in transgenic plants. Furthermore, exogenous spermidine, spermine and spermidine synthesis inhibitor dicyclohexylamine were used for complementary tests, which resulted in small leaves and dwarf plants, big leaves and early flowering, and big leaves and dwarf plants, respectively. these results indicate that spermidine and spermine are mainly involved in the vegetative growth and early flowering stages, respectively. Expression analysis of flowering-related genes suggested that NtSOC 1 , NtAP 1 , NtNFL 1 and NtFT 4 were upregulated in transgenic plants. In conclusion, ectopic GhSAMDC 1 is involved in the conversion of spermidine to spermine, resulting in rapid vegetative growth and early flowering in tobacco, which could be applied to genetically improve plants. Polyamines, including putrescine (diamine), spermidine (triamine), and spermine (tetraamine), play various roles in plants 1,2. Many reports indicate that polyamines are affect the fluidity of the lipid membrane and participate in the biotic and abiotic stress responses 3-9. Furthermore, polyamines have been reported to be involved in various physiological processes 9-14. The polyamine metabolic pathways have been elucidated in plants 15. Putrescine originates from ornithine or arginine, catalysed by ornithine decarboxylase, or arginine decarboxylase, agmatine iminohydrolase and N-carbamoyl putrescine amidohydrolase. Spermidine and spermine are derived from putrescine and are catalysed by spermidine and spermine synthases, respectively. S-adenosylmethionine decarboxylases (SAMDCs) catalyse the S-adenosylmethionine decarboxylation reaction and provide an aminopropyl group, which is involved in spermidine and spermine synthesis. SAMDC transcripts have been analysed in a wide variety of plant species, often with higher transcript levels detected in reproductive than vegetative organs 16-21. Further evidence that polyamines are required for growth and development comes from analysing the Arabidopsis thalian bud2 mutant. This mutant has an inactivated AtSAMDC 4 gene and an enlarged vascular phenotype 22. Moreover, AtSAMDC 1 is involved in an interaction between beet severe curly top virus and Arabidopsis plants 23. Numerous studies have reported upregulation of SAMDC in response to various stressors, including drought, salt, high or low

show abstract

Characterization of the Polyamine Biosynthetic Pathways and Salt Stress Response in Brachypodium distachyon

Cited by 40 publications

References 39 publications

Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

Transgene Pyramiding of Salt Responsive Protein 3-1 (SaSRP3-1) and SaVHAc1 From Spartina alterniflora L. Enhances Salt Tolerance in Rice

Ectopic expression of GhSAMDC1 improved plant vegetative growth and early flowering through conversion of spermidine to spermine in tobacco

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